氨氮对厌氧颗粒污泥产甲烷菌的毒性研究于芳芳

2008,Vol.25No.4　Chemistry&Bioengineering75　　　:2007-11-05:于芳芳(1982-),女,山东烟台人,硕士研究生,研究方向为水污染控制;通讯联系人:伍健东,副教授。E-mail:[e-mail],[e-mail]。,(华南理工大学环境科学与工程学院,广东广州510006)　　　:在中温(35±1)℃厌氧条件下,以葡萄糖为有机碳源,采用间歇实验方法研究了氨氮对厌氧颗粒污泥产甲烷菌的毒性。结果表明,当COD为6000mg·L-1时,氨氮对产甲烷菌产生毒害作用的浓度为1500mg·L-1,IC50为4000mg·L-1,毒性周期的最大产甲烷速率为92mL·h-1,氨氮的平均去除率为13.17%;去除高浓度氨氮废水后,厌氧颗粒污泥产甲烷活性有不同程度的恢复,在毒性实验中受到的抑制作用越小,产甲烷活性恢复得就越快、越完全。此外,研究表明,适当提高系统的碱度和污泥浓度可以降低氨氮对厌氧颗粒污泥产甲烷菌的毒性。:氨氮;IC反应器;厌氧颗粒污泥;产甲烷菌;毒性:X703　　　　　:A　　　　　:1672-5425(2008)04-0075-04　　,,,、,,[1,2]。,,,[3]。,,,,,。COD,IC,。1　1.1　,C∶N∶P200∶5∶1,Fe、Co、Ni(1)。NH4Cl()。1　Tab.1Compositionandcontentsoftraceelements/mg·L-1/mg·L-1EDTA5000CuSO4·5H2O250CaCl2330MgSO4550NiCl2·6H2O190CoCl2·6H2O240H3BO414MnCl2·4H2O990ZnSO4·7H2O430NH4MoO4·4H2O91.2　(1)1　Fig.1　Schematicdiagramofexperimentaldevice。125mL100mL:/2008476　　　,25mL。1,35℃。,。,,,,。1.3　12LIC,,(TS)20g·L-1,0.5～5mm,0.9712,1.0358。2。2　Fig.2　TheSEMpicturesofanaerobicgranularsludge2,,,[4]。,,,,[5]。1.4　[6]COD:;pH:;SSVSS:;:;:,3%NaOH,、。2　2.1　[7～11](),,,,[7]。COD6000mg·L-13。3,500mg·L-1800mg·L-1,3　Fig.3　Effectofammoniumconcentrationonmethanogenicactivityofanaerobicgranularsludge,5%9%;1500mg·L-1,;3000mg·L-14000mg·L-1,,33%50%;4000mg·L-1,,;,;50%,IC504000mg·L-1。,800mg·L-1,,3.06L·L-1·d-1;,,92mL·h-1(4),,,6h,15.5mL·h-1,,6h。4　800mg·L-1Fig.4　Methanogenicvelocityof800mg·L-1ammonium2.2　,,,。5。5,IC:/2008477　　　5　Fig.5　Removalefficiencyofammonium,,13.17%,,。2.3　,,。,COD6000mg·L-1。6。6　Fig.6　Accumulatedmethanogenicquantityinthetoxicityrecoveryperiod6,1500mg·L-1,,;3000～5000mg·L-1,,。2.4　COD8000mg·L-1、2000mg·L-1、,(TSS),7、8。7,,;,pH7.01,pH5.9,7　Fig.7　Effectofalkalinityonmethanogenicactivity8　Fig.8　Effectofsludgeconcentrationonmethanogenicactivity。8,。3　(1):,,COD6000mg·L-1,1500mg·L-1,IC504000mg·L-1,92mL·h-1。(2),IC,13.17%。(3),,,、。(4),。:[1]　.[J].:/2008478　　　,1997,13(S):19-21.[2]　HansenKH,AngelidakiI,AhringBK.Anaerobicdigestionofswinemanure:Inhibitionbyammonia[J].WatRes,1998,32(1):5-12.[3]　,.[J].,2002,28(2):93-97.[4]　,.[J].,1995,1(3):252-259.[5]　BaberWP,StuckeyDC.Start-upStrategiesforAnaerobicBaf-fledReactorsTreataSynthenicSucroseFeed[M].In:Proc.8thInternationalConferenceonAnaerobicDigestion,1997:40-47.[6]　《》.()[M].:,2002:102-105,211,279-281.[7]　,.34[J].,1992,12(4):250-254.[8]　,,.[J].(),2005,45(9):1294-1296.[9]　BorjaR,SanchezE,WeilandP.Influenceofammoniaconcentra-tiononthermophilicanaerobicdigestionofcattlemanureinup-flowanaerobicsludgeblanket(UASB)reactors[J].ProcessBio-chemistry,1996,31(5):477-483.[10]　SossaK,AlarconM,AspeE,etal.EffectofammoniaonthemethanogenicactivityofmethylaminotrophicmethaneproducingArchaeaenrichedbiofilm[J].Anaerobe,2004,10(1):13-18.[11]　GerardiMH.TheMicrobiologyofAnaerobicDigesters[M].Hoboken,NewJersey,USA:JohnWileyandSons,2003:158-161.ToxicityStudyofAmmoniumonMethanogenicBacteriainAnaerobicGranularSludgeYUFang-fang,WUJian-dong(CollegeofEnvironmentalScienceandEngineering,SouthChinaUniversityofTechnology,Guangzhou510006,China)Abstract:Thetoxicityofammoniumonmethanogenicbacteriainanaerobicgranularsludgewasinvestiga-tedusingintermittentexperimentsundertheconditionofmediumtemperatureof(35±1)℃,withglucoseasorganiccarbonsource.Theexperimentresultsshowedthat,whenCODwas6000mg·L-1,themethanogenicbacteriawereinhibitedattheammoniumconcentrationof1500mg·L-1withthecorrespondingIC50of4000mg·L-1andtheperiodicalmaximalmethanogenicvelocityof92mL·h-1,andtheaverageammoniumremovalratewas13.17%.Whenthehighconcentrationofammoniumwasremoved,themethanogenicactivityrecov-eredinvaryingdegrees,andthesmallertheinhibition,thefastertherecoveryofmethanogenicactivityofsludge.Furthermore,theresultsalsoindicatedthat,whenalkalinityandsludgeconcentrationbeingenhanced,thetoxicityofammoniumonmethanogenicbacteriainanaerobicgranularsludgedecreased.Keywords:ammonium;internalcirculationreactor;anaerobicgranularsludge;methnogenicbacterium;toxicity(上接第59页)OxidationDamageofDi-(2-ethylhexyl)phthalatetoBrainandKidneyCellsofGoldLarvalFishesLIXue-bin,CHENLi,WUDan,LIYan,DINGShu-mao,YANGXu,YUANJun-lin(LaboratoryofEnvironmentalScience,CollegeofLifeScience,HuazhongNormalUniversity,Wuhan430079,China)Abstract:Theoxidationdamagesofdi-(2-ethylhexyl)phthalate(DEHP)tobrainandkidneycellsofgoldlarvalfishesandthegenerationmechanismswereinvestigated.Theresultsshowedthat,DEHPcouldresultedintheoxidationdamagesofbrainandkidneycellsofgoldlarvalfishes.WiththeincreasementofDEHPconcentra-tion,theactivityofsuperoxidedismutasefirstlyrisedandthendropped,andthemalondialdehydecontentrisedallthewhile,whichshowedobviousdose-responserelationship.Keywords:di-(2-ethylhexyl)phthalate;goldlarvalfish;superoxidedismutase(SOD);malondialdehyde(MDA)