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40420154ENVIＲONMENTALSCIENCEANDMANAGEMENTVol.40No.4Apr．20152015－02－2320120461983－。1674－6139201504－0044－051221．2115002．210094、pH、DO、、。22.8mg/L·h80%32.6。30℃、pH=8.5、DO=0.5mg/L、HCO－3/NH+4－N=1．67、400mg/L。X703．1AStudyonEnrichmentCultureandInfluencingFactorsofAmmoniaOxidizingBacteriaDaiXin1WuYajie2AnLichao21．NanjingKeshengEnvironmentProtectionCo．LtdNanjing211500China2．SchoolofEnvironmentalandBiologicalEngineeringNanjingUniversityofScienceandTechnologyNanjing210094ChinaAbstractInthispaperenrichmentprocessofammonia－oxidizingbacteriaisstudiedundertheconditionsofintermittenttrainingmethods．Thispapermainlydiscussestheinfluenceofammoniaoxidizingbacteriagrowthconditionsonshortcutnitrifica-tionsuchastemperatureinitialpHvalueDOalkalinitytheinfluentammoniaconcentrationetc．Experimentalstudieshavefoundthatwhenammoniumoxidizingbacteriaenrichedprocessisfinishedthetreatmentindexstabilizeswithammoniaoxidationratereached22.8mg/L·hnitriteaccumulationrateofaround80%thenumberofammonia－oxidizingbacteriamayberaisedto32．6－foldenrichmentbefore．Inadditionontheinfluencefactorsoftheparalleltestfoundthatwhenthetemperatureis30℃pH=8.5DO=0.5mg/LHCO－3/NH+4－Nmolarratiois1.67andtheinfluentammonianitrogenconcentrationislessthan400mg/Listheoptimumgrowthconditionsfortheammoniaoxidizingbacteria．Undertheseconditionsitisthemostconducivetotherealizationofshortcutnitrification．Keywordsammoniaoxidizingbacteriaenrichmentcultureinfluencingfactorsshortcutnitrification“”。、、。NH+4－NNO－2－NNO－3－NNO－2－NN21。25%、60%、40%50%2－3。。。·44·40420154·Vol.40No.4Apr．201511．11．1．1。1．1．22L、、12h。1。11L。1．1．3。300mg/L250mg/LNaHCO33000mg/LK2HPO4·12H2O500mg/L。1．21．2．112h。5min11h50min5min1L。250mg/LNH4Cl1146mg/LNaHCO33000mg/LK2HPO4·12H2O500mg/LFeSO4·7H2O30mg/LMgSO4·7H2O30mg/L。30℃DO1.0mg/L。MLSS3000mg/L。1．2．2MPN4AOB。4。NH42SO42g/L、K2HPO40.75g/L、NaH2PO40.25g/L、MnSO4·4H2O0.01g/L、MgSO4·7H2O0.03g/L、CaCO35g/L、pH=7.2。25℃14dgriessMPN1。1．2．3、pH、DO、、、。1．2．4η1mg/L·dη1η1=CNH+4－N1－CNH+4－N2/tCNH+4－N1─mg/LCNH+4－N2─tmg/Lt─h。1．2．5η2%η2η2=CNO－2－NCNO－2－N+CNO－3－N×100%CNO－2－N─mg/LCNO－3－N─mg/L。1．2．65NO－2－NN－1－－NO－3－NBDOYSI550ApHPHS－25pH。22．1300mg/L600mg/L。2、3。2·54·40420154·Vol.40No.4Apr．20153、2、35。。90%80%。2．2DO=1.0mg/L30℃pH=8.0、、4。4480%。。11h22.7mg/L91.7%22.8mg/L·h。2．3MPN25dMPN1．2×107/gMLSS3.91×108/gMLSS32.6。2．4pH=8.0DO=1.0mg/L=300mg/LHＲT=12h、。5。55。15℃164.71mg/L32.43mg/L49.0%≥20℃210mg/L85%。20℃NOBAOB20℃AOBNOB。AOBNO－26。30℃35℃12h·64·40420154·Vol.40No.4Apr．201590%30℃93.2%82.23%。30℃。2．5pHT=30℃DO=1.0mg/L300mg/LHＲT=12h、pH。6。6pH6pHpH=6.561.3%pHpH=8.599.32%272.27mg/L。pHpH＞9.5pH1064.4%。FAAOBNOBAOB10mg/L～150mg/LNOB0.1mg/L～1.0mg/L。300mg/LpHFA。pH8.5FA76．1mg/LpH9.0FA160mg/LAOBpHAOBpH9.5～10。pH8.5。2．6DOT=30℃pH=8．5mg/L300mg/LHＲT=12h、DO。7。7DO7DO90%80%。DO0.5mg/L96.7%DO2.5mg/L94.1%。AOBNOB7AOBNOBpH6．3NOB80%。BALMELLEDONO－2－N。DO0.5mg/L。2．7T=30℃pH=8．5mg/LDO=0.5mg/L300mg/LHＲT=12h、。8。·74·40420154·Vol.40No.4Apr．201588。893mgCaCO3/L60.4%pH6.01786mgCaCO3/L97.9%pH6.5。。1786mgCaCO3/LNaHCO33000mg/L。HCO－3/NH+4－N=1.67。2．8T=30℃pH=8．5mg/LDO=0.5mg/LHCO－3/NH+4－N=7.26HＲT=12h、。9。99100mg/L400mg/L90%500mg/L12h74.4%。80%。3180%。222.8mg/L·h。3MPN32.6。430℃、pH=8.5、DO=0.5mg/L、HCO－3/NH+4－N=1.67、400mg/L。1．M．2006．2XinZhangDaijunZhangQiangHeetal．Shortcutnitrification－denitrificationinasequencingbatchreactorbycontrollingaerationdurationbasedonhydrogenionproductionrateonlinemonitoringJ．EnvironmentalTechnology201435121478－1483．3L．WangJ．ZhuC．Miller．ShortcutNitrificationandDenitrificationofSwineWastewaterinaSequencingBatchＲeactorSystemJ．TransactionsoftheASABE2010533813－818．4GB/T26322－2010．MPNS．2010．5．M．2002．6HyungseokYoo．Nitrogenremovalfromsyntheticwastewaterbysimultaneousnitrificationanddenitrificationvianitriteinanintermittently－aeratedreactorJ．WaterＲesearch1999331146－149．7WiesmannU．BiologicalnitrogenremovalfromwastewaterM．Biotechnics/Wastewater．SpringerBerlinHeidelberg1994113－154．·84·