氨氧化细菌富集培养的实验研究章正勇

2007,Vol.24No.7　Chemistry&Bioengineering63　　　:2007-03-28:章正勇(1983-),男,江苏南京人,硕士研究生,主要从事环境工程技术方面的研究;通讯联系人:安立超,教授。E-mail:[e-mail]。,　,(南京理工大学环境科学与工程系,江苏南京210094)　　　:建立了一种氨氧化细菌的富集培养方法。采用该技术培养30d,氨氧化细菌能够富集达到90%的亚硝酸盐累积率。同时进行了扩大规模的实验,结果也验证了该技术的可行性,为短程硝化—反硝化的实际应用提供了依据。:氨氧化细菌;亚硝酸盐累积率;短程硝化—反硝化:X703.1　Q93-335　　　　:A　　　　:1672-5425(2007)07-0063-02　　—,O2、,。—。,,。-。[1～3]、pH、、、、。、,,()。、、pH,,,-。1　1.1　(1),5L。,;100W,,;,pH7.5～8.5。1.2　SBR。1　Fig.1　Theschematicdiagramofexperimentalapparatus(g·L-1):(NH4)2SO41.0～1.5;NaHCO32.5;NaCl2.0;K2HPO40.50～0.75;FeSO4·7H2O0.20～0.40;MgSO4·7H2O0.25～0.50;MLSS10。1.3　:5L,24h,(30±1)℃、pH7.5～8.5、0.5mg·L-1。,(NH4)2SO41.0g·L-1,15d,,(NH4)2SO41.5g·L-1,。:,50L,。1.4　DOThermoOrion850A;pHPH-3;:/2007764　　　[4];N-(1-)-[4];[5]。2　,,。2.1　2.1.1　(2)2　Fig.2　Thevaryingcurvesofinletconcentration,outletconcentrationandremovalrateofNH3-N2,15d,,52%73%;16d,,3d,50%,,,80%。。2.1.2　(3)3　、Fig.3　Thevaryingcurvesoftheincrementofoutletnitrite,nitrateconcentrationandcumulativerateofnitrite,。3,40%,15d,,61%;16d,,90%,。,。,。,,,。2.2　(4、5)4、5,,90%,。,、,。(下转第67页)　:/2007767　　　,20min,,30min。30min。3　,pH4.4、H2O22∶1、30min。,。:[1]　.[J].,1998,18(2):4-6.[2]　,,,.N2O,Fe-P[J].,2003,17(5):342-346.[3]　,,.-4-[J].(),2003,41(2):238-240.[4]　NiceJA,BewtraJK,TaylorKE,etal.Enzymecatalyzedpoly-merizationandprecipitationofaromaticcompoundsfromwastewater[J].WatSciTech,1992,25(3):157-164.[5]　DuranN.Potentialapplicationsofoxidativeenzymesandphenoloxidase-likecompoundsinwastewaterandsoiltreatment.Are-view[J].ApplCatalB,2000,28(2):83-89.StudyontheRemovalofPyrocatecholfromWasteWaterbyHorseradishPeroxidaseZHENGQi,LIZhong-ming(CollegeofChemistryandEnvironmentalEngineering,JianghanUniversity,Wuhan430056,China)Abstract:Treatmentofwastewatercontainingpyrocatecholcatalyzedbyhorseradishperoxidase(HRP)wasstudied.TheresultshowedHRPcouldremovepyrocatecholeffectively.Theoptimumconditionswerede-terminedasfollows:pHvalue4.4,molarratioofH2O2topyrocatechol2∶1,reactiontime30min.Moreover,increasingthedosageofHRPappropriatelycouldshortenreactiontimeobviously.Keywords:horseradishperoxidase;pyrocatechol;enzymecatalyticreaction(上接第64页)3　,90%,,—。,。:[1]　,,.DOpH[J].,2006,7(1):37-41.[2]　,,.[J].,2004,24(5):618-622.[3]　,,.—[J].,2000,16(2):29-31.[4]　《》.(4)[M].:,2002.[5]　,,.[J].,1999,16(1):41.ExperimentalResearchonEnrichmentTechniqueforAmmoniaOxidizingBacteriaZHANGZheng-yong,CHENXu,ANLi-chao(DepartmentofEnvironmentalScienceandEngineering,NanjingUniversityofScienceandTechnology,Nanjing210094,China)Abstract:Anenrichmenttechniqueforammoniaoxidizingbacteriawasintroducedinthispaper.Witham-moniaoxidizingbacteriaenrichedafter30daysusingtheenrichmenttechnique,thenitriteaccumulationratecouldreach90%.Thelarge-scaleexperimentwasdoneandtheresultvalidatedthefeasibilityoftheenrichmenttechnique,soitprovidedimportantevidenceforpracticalapplicationofshortcutnitrificationanddenitrifica-tion.Keywords:ammoniaoxidizingbacteria;nitriteaccumulationrate;shortcutnitrificationanddenitrification