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:2005-02-16:“973”,104250:(1978-),,,。StudyonDegradationofQuinolineinCokingPlantWastewaterUsingWhiteRotFungi任大军1 张晓昱2 颜克亮2 吴晓辉1 陆晓华1(1.华中科技大学环境科学研究所 武汉 430074);(2.华中科技大学生命科学与技术学院 武汉 430074) 选用白腐菌BP降解喹啉,研究了白腐菌在不同培养基中对喹啉的降解过程和机理,以及喹啉的降解与白腐菌漆酶活力、生物量比增长速率、培养基pH值的关系。结果显示秸秆滤出液培养基中生长的白腐菌对喹啉具有最高的去除率89%;喹啉的相对去除率与白腐菌漆酶活力、生物量比增长速率具有较好的相关性;白腐菌BP最适pH为6～7;2-羟基喹啉是喹啉降解过程中首先出现的中间产物。 Abstract Whiterotfungus(BP)wasselectedtodegradequinoline.Theresearchincludedthedegradationprocessandmechanismofquinolineindifferentculturemediums,therelationsofdegradationofquinolinewithactivityoflaccase,thespecificincreaserateofbiomassandpHofculturemedium.Resultsshowedthat89%ofquinolinewasdegradedbywhiterotfungiintheculturemediumofstrawleachingliquid.Therelativedegradationrateofquinolinewasclosecorrelatedwiththeactivityoflaccase,specificincreaserateofbiomass.TheoptimalpHconditionforWhiterotfungus(BP)was6-7,2-OH-quinolinewasthefirstintermediateinthebiodegradationprocessofquinoline.Keywords WhiteRotFungi CokingWater Quinoline; ,、,,,。,,、。,。[1],,,;、、pH,,[2];BP,。1 1.1 BP,。:,,KH2PO4,K2KPO4,MgSO47H2O,NaCl,CaCl22H2O,MnSO4H2O,FeSO47H2O,ZnSO47H2O,CuSO45H2O,VB1;:,;:,,。1.2 ,—20— 32 1 20062DOI:牨牥牨牰牳牥牫牤jcnkiissn牨牥牥牬牰牪牨牰牪牥牥牰牥牨牥牥牱1108mg/L。10mL100mL250mL,,(150r/min),25℃。1.3 :HitachiHPLC()。:(1%)(50:50,v/v),0.6mL/min,313nm,20uL。:UV-754。mLmin0.001。2 2.1 1 1,89%,78%,70%。,,,。,0～9d,,,;9～15d,,,。2.2 2.2.1 漆酶的影响 2,,9d;,,6d。2 ,6～9d,(:=/15d,。)30%22%。3d9d,20%～30%,。,,14%。6d、9d,、,。2.2.2 生物量的影响 3,0～6d,6d(=/3d);9d;6d、9d,、,,。—21— 3 2.2.3 pH值的影响4 pHpH,4,,pH,、pH4.86～5.7、6.45～7.976.15～6.88。,pH,、,pH5.74、6.65、7.05。BP2pH6～7,5～8。2.3 5,6.2min。6、78,15d,,,:。3.7min、4.2min6.9min,。BP,H2O2OH,OH,,。2,[3,4]。W.jianlong[5]GC/MS,2-,2-,ShuklaOP[6],2,8-;3.7min4.2min,2-———;6.9min[3,4],,,。5 HPLC(5)6 15dHPLC(5)7 15dHPLC(5)—22— 32 1 200628 15dHPLC(5)3 (1),89%;(2)、,、;(3)BPpH6～7,5～8;(4)2-,。 1.M.G.Kenneth,E.Hammel.InitialStepsintheDegradationofMethoxychlorbytheWhiteRotFungusPhanerochaetechrysos-porium[J].Appl.Environ.Microbial,1997,63(3):1175～1177.2.,.[J].,1999,86(3):8～12.3.SchachS,SchwardG,FetznerSetal.MicrobialMetabolismofQuinolineandRelatedCompoundsⅩⅦDegradationof3-MethylquinolinebyComamunastestosteroni63[J].Biol.ChemHoppe-Seyler,1993,(374):175～181.4.SchwarzG,BauderR,SpeerMetal.MicrobialMetabolismofQuinolineandRelatedCompoundsⅡ.DegradationofQuinolinebyPseudomonasFluorescens3,PseudomonasPutida86,andRhodococcusSpecB1.Biol.ChemHoppe-Seyler,1989(370):1183～1189.5.W.jianlong,Qxiangchun,Hlipingetal.MicrobialDegrada-tionofQuinolinebyImmobilizedCellsofBurkholderiaPickettii[J].WaterResearch,2002(36):2288～2296.6.ShuklaOP.MicrobialTransformationofQuinolinebyaPseudo-monassp[J].ApplEnvironMicrobiol,1986(51):1332～1342.(上接第13页)7 PM10SO2%%%33335736536136335691.294.896.094.995.594.536035636536435836192.694.597.093.792.194.0 1..(ADMS)[J].,2004,30(1):67～69.2.,,.[M].:,1985.—23—