白腐真菌处理皂素生产废水的研究

3412()Vol.34No.12200612Jour.ofNorthwestSci2TechUniv.ofAgri.andFor.(Nat.Sci.Ed.)Dec.2006X,,,,(,712100)[],,CODCr20000mgöL,KH2PO410göLMgSO40.05göL2.0göL,pH5.028150mLöL4d120römin,CODCr80%,10%,28.2g;,[];;[]X505[]A[]167129387(2006)1220152207(Yam)(Dioscoreai2ingiberensisC.H.Wright),(Diosgenin)200,,,,,[1][2],,:(1),,,;(2),;(3)(),,;(4),,,SCP,,[3]DDTTNTCCl4,[4],,,11.11.1.1,1.1.2(Phanerochaetechrysospori2um)1.1.3B1,1.1.4(),LSB250L(),Pyx2PHS2X21(),CHA2S(),SP21105(X[]2005211224[]2004[](1970-),,,,,E2mail:[e-mail][](1953-),,,,,),HH26(),800(),pHS23C()1.256PDA(200g20g15g,pH6.0),2823d[5]1.3,23100mLPDA(200g20g3g1.5gVB1,pH6.0)250mL,28120römin3OD650pH,OD650pH,OD650[6],1.42,4,6,PDA30%,60%,85%100%,NaOHpH6.0,250mL100mL,100mLöL,OD650CODCrpH,100mLöL100%,m(COD)m(N)m(P)=20051,pH6.0[7],4dCODCrpHOD650,CODCrOD650,,1.5,,CODCr,pH3,()(KH2PO4)(MgSO4)3,,3,3[8],121Table1Externalfactorsandlevelsoforthogonaloptimi2zingexperimentsonsaponinManufacturwastewaterdisposalbywhiterotfungusLevelFactorspHAö(mLL-1)InoculationamountBödDisposaltimeC1234.05.06.0501001502342Table2InternalfactorsandlevelsoforthogonaloptimizingexperimentsonsaponinmanufacturingwastewaterdisposalbywhiterotfungusLevelFactorsKH2PO4ö(gL-1)AMgSO4ö(gL-1)Bö(gL-1)AmmoniumtartrateC1230.11.010.00.050.505.000.22.020.01.6,SBRUASB,1.7CODCr[9],[9],pHpHS23C,OD650nm[10],:CODCrö%=[(CODCr-CODCr)öCODCr]100%;ö%=[(-)ö]100%;OD650ö%=[(OD650-OD650)öOD650]100%;ö%=[(-)ö]100%22.1pH135112:1Fig.1Growthofwhiterotfungus1,,,23d1,45d2,45d,5dOD650,9dpH,,pHpH5.5,2.22.2.1CODCrCODCr22,46CODCr70%,2CODCr27.4%,CODCr,2,,62000025000mgöL,,,,6CODCr2000025000mgöL,CODCr23%35%[11212],CODCr,CODCr700012000mgöLCODCr23,CODCr232.2.2,32CODCr--.2(CODCr4000045000mgöL);--.4(CODCr3000035000mgöL);--.6(CODCr2000025000mgöL)Fig.2ImpactoftamedwhiterotfungusonCODremovalrateofmanufacturingwastewaterfromdifferentdilutemultiplesaponin--.Twodilutemultiples(ThestartCODcr=40000-45000mgöL);--.Fourdilutemultiples(ThestartCODCr=30000-35000mgöL);--.Sixdilutemultiples(ThestartCODCr=20000-25000mgöL)3--.2(=10000);--.4(=8000);--.6(=3000)Fig.3Impactoftamedrotfungusonthecolorremovalofmanufacturingwastewaterfromdifferentdilutemultiplesaponin--.Twodilutemultiples(Thestartcolordegree=10000);--.Fourdilutemultiples(Thestartcolordegree=8000);--.Sixdilutemultiples(colordegree=3000)451()343,2,,50%,2d,,,,2d2.2.3pH,pH33pHTable3ImpactoftamedwhiterotfungusonthepHvalueofwastewaterfromdifferentdilutemultipleofsaponinmanufacturingDilutemultipleödDisposaltime012342645664646886646883,pH1,23,4463pH,34CODCr,,pH,2.2.4OD650OD65044OD650--.2;--.4;--.6Fig.4ImpactofwhiterotfungusontheincreaserateofOD650inthewastewaterfromdifferentdilutemultipleofsaponinmanufacturing--.Twodilutemultiples;--.Fourdilutemultiples;--.Sixdilutemultiples4,3d,OD650,4,2OD65046,24646,,,6(),34d2.33CODCr4,CODCr:pH,CODCrA2B3C328120römin,CODCr:pH5.0,150mLöL,4d,3,,CODCr24648.36mgöLCODCr85.5%,9.1%,42.5göL,CODCr15%,CODCrA2B3C13CODCr24648.36mgöL,A2B3C1CODCr20100.05mgöL,2CODCr,2CODcr,CODCr55112:4Table4ResultsoforthogonaloptimizingexperimentsofexternalfactorsonsaponinmanufacturingwastewaterdisposalbywhiterotfungusNopHAö(mLL-1)InoculationBödDisposaltimeCThevacantrankVijkCODCrö%CODCrremovalrate1234567891(4.0)1(4.0)1(4.0)2(5.0)2(5.0)2(5.0)3(6.0)3(6.0)3(6.0)1(50)2(100)3(150)1(50)2(100)3(150)1(50)2(100)3(150)1(2)2(3)3(4)2(3)3(4)1(2)3(4)1(2)2(3)12331223178.1675.5782.9767.8579.7392.0775.5758.7162.95K1K2K3236.7239.65197.23221.58214.01237.99228.94206.37238.27220.84243.21209.53673.58k1k2k378.90079.88365.74373.86071.33779.33076.31368.79079.42373.61381.07069.843R14.1407.99310.63311.2272.4KH2PO4MgSO4CODCr5,3CODCr:MgSO4KH2PO4,A3B1C228120röminpH5.0150mLöL4d,KH2PO410göL,MgSO40.05göL,2.0göL,CODCr,3,,CODCr38705.33mgöLCODCr83.41%,10%,28.2göL,,,,CODCr37.47%,45.94%,15.0göL,5Table5ResultsofinternalfactorsoforthogonaloptimizingexperimentsonsaponinmanufacturwastewaterdisposalbywhiterotfungusNo.KH2PO4ö(gL-1)AMgSO4ö(gL-1)Bö(gL-1)AmmoniumtartrateCThevacantrankVijkCODCrö%CODCrremovalrate1234567891(0.1)1(0.1)1(0.1)2(1.0)2(1.0)2(1.0)3(10)3(10)3(10)1(0.05)2(0.5)3(5.0)1(0.05)2(0.5)3(5.0)1(0.05)2(0.5)3(5.0)1(0.2)2(2.0)3(20)2(2.0)3(20)1(0.2)3(20)1(0.2)2(2.0)12331223166.6565.0543.8365.7653.9939.4065.7273.6052.46K1K2K3175.53159.15191.78198.13192.64135.69179.65183.27163.54526.46k1k2k358.51053.05063.92766.04364.21345.23059.88361.09054.51357.70056.72361.063R10.87720.8136.5774.340651()342.5CODCr6,CODCr81.40%,6Table6ResultsofsecondleveltreatmentexperimentsonsaponinmanufacturingwastewaterbytamedwhiterotfungusMethodoftreatmentCODCrö(mgL-1)OD650StartAfterhandlingö%RemovalrateStartAfterhandlingö%Increaserate+Aerobicsludge+whiterotfungus7388.511374.1081.401.6933.990135.68+Anaerobicsludge+whiterotfungus7645.865308.2030.571.7224.500161.32MethodoftreatmentThecolordegreepHö(gL-1)ThecellalbumenincreaserateStartAfterhandlingö%ColorremovalrateStartAfterhandlingStartAfterhandlingö%Increaserate+Aerobicsludge+whiterotfungus3000250016.675.07.295.813.3129.31+Anaerobicsludge+whiterotfungus3250250023.085.05.868.920.8133.7131)CODCr2000030000mgöL,CODCr23,2),3):CODCr2