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,＊,,,,(, 200240):,PTA,.,136d,COD3kg·(m3·d)-1,3～4d,PTACOD75%～85%,.,(EPS)、DNA,,.:;;;PTA:X703.1 :A :0250-3301(2009)10-2946-06:2008-11-27;:2009-01-22:(50808121):(1986～),,,,E-mail:wu-bingtao@yahoo.cn＊,E-mail:weilizhou@sjtu.edu.cnStart-upCharacteristicsoftheAnaerobicReactorSeededwithImmobilizedMicroorganismsWUBing-tao,ZHOUWei-li,ZHANGZhen-jia,CHENGXue-hang,DONGYa-mei,CHILi-na(SchoolofEnvironmentalScienceandEngineering,ShanghaiJiaotongUniversity,Shanghai200240,China)Abstract:Inordertoovercomethedisadvantagesoftheanaerobicreactorsuchasslowgrowthandlongstart-up,theflocculentanaerobicsludgewasembeddedandusedastheseedsludgeintheanaerobictreatmentofPTAwastewaterwiththeobjectiveofkeepingbiomassinthereactor.Thestart-upcharacteristicsoftheUASBreactorwereinvestigated.Duringthe136days'running,CODremovalrateofPTAwastewaterachieved75%-85%atthevolumetricloadingrate(COD)of3kg·(m3·d)-1andthehydraulicretentiontime(HRT)of3-4day.Theanaerobicsystemhadgoodstabilityandbiomassretainingability.Ontheotherhand,variationsofEPS,SEMobservationandmethanogensDNAinsludgegranulesverifiedthegrowthofimmobilizedbacteriainbothquantityandmicroorganismmorphology,althoughmasstransferthroughtheimmobilizationmediawastosomedegreelimited.Keywords:embedding;immobilization;anaerobicreactor;PTAwastewater .,,,,,[1].,[2],.,.[3],.[4],99%,90.5%.,,.[5](LAS),,.[6](SBR),.,,,,,.3010200910 ENVIRONMENTALSCIENCEVol.30,No.10Oct.,2009DOI:10.13227/j.hjkx.2009.10.0191 1.1 (PTA).1.,BOD5COD0.6～0.7,,COD,.(GC-MS2).pH,(N、P).,PTA,Mn、Co,.1 PTAmg·L-1Table1 Characteristicsoftherawwastewatermg·L-1℃35～40SS188VSS106 pH5.5～6.5BOD54100～5200Mn14～20COD5900～7800TN0.30～2.17Co15～252 PTAGC-MSTable2 ComponentsofPTAwastewaterthroughGC-MSanalysis%11.045.2917.8842.944.21.2 1.UASB16L,110mm,1.7m,36℃±1℃.UASB,,,.,3mm.,,NaHCO3pH6.0～6.5.1.3 pH、COD、(VFA);(10cm)(EPS)DNA.COD(GB11914-89);(HZ-HJ-SZ-0130);pHpH;VFA1 Fig.1 Experimentallayout [7];(EPS)[8],EPSFolin[9],-[10,11];-DNA[12,13],PCR,DNA;,,,,(SEM,JEOL:JSM-6360LV).2 2.1 3,2.,COD2000mg·L-1,(HRT)3～4d,COD1kg·(m3·d)-1.13d,,COD,(6.40%～38.95%).,.14d,VFA,COD,.17d,COD50%(35.9%～53.4%),VFA,.17～27d,COD50%,,,28d,(COD)1000mg·L-1.28～32d,COD75%,.294710: 3 Table3 RunningparametersoftheanaerobicreactorseededwithimmobilizedmicroorganismsdCODmg·L-1L·d-1HRTdCODkg·(m3·d)-1CODmg·L-11～1219504.7～7.02.3～3.80.57～0.9413～2720004.4～4.93.5～4.10.55～0.6828～3630004.5～4.83.5～3.80.84～0.90100037～4040003.9～5.03.4～4.30.98～1.25100041～5250003.4～6.43.0～5.01.06～2.01100053～6155004.0～4.63.7～4.21.38～1.57150062～757092～75503.9～7.63.5～4.31.83～3.38150076～907777～86933.5～5.23.2～4.91.81～2.83200091～1046777～83503.9～6.52.5～4.11.70～3.361000105～1147331～74604.7～5.43.0～3.42.21～2.46115～11981675.2～6.02.7～3.12.67～2.96120～1296300～65345.7～7.92.0～3.22.06～3.10130～1367260～80326.3～7.82.1～2.62.87～3.542 Fig.2 Runningperformanceofthereactor ,,COD,,,.62d,,,COD,70d65%,1,(COD1500mg·L-12000mg·L-1),.,75%,.PTA.91d103d,,COD,VFA,.,75%～80%.136d,(126d),COD3kg·(m3·d)-1,126d2.0d,COD50%,.,130d2.5～3.0d,COD60%.,,3.0d.PTAPTA.,,,.,,.,VFA,2948 30.HRT,VFA.,.pH,pHOH-,pH.,7.0,.,SS,.,.2.2 .UASB,3mm.、.(3),[3(a)],,.,.84d,[3(b)],,[3(c)].,.,,.,,.、,.128d,,[3(d)][3(e)][3(f)].,PTA.,.3 Fig.3 Changesofthemicroorganismmorphology294910:2.3 (EPS),[14～16].EPS,TOC70%～80%[17].,[18～21]、[22]、、,[23].EPS,,,[24,25].,EPS.[26],,,EPS,.[27,28]、、[29]EPS.,,EPS.,EPS.EPS.4,EPS(0.202mg·cm-3).12d,EPS,..4 Fig.4 Variationofextracellularpolymericsubstancesinthesludge 13～26d,EPS,,..28d,EPS.,,,EPS.,,,.,,EPS,,.70d,,,,,.90d,,,,,.2.4 DNA,.DNADNAPCR.5.,,21d.1:;2:;3:21d;4:35d;5:49d;6:70d;7:84d;8:99d;9:115d;10:127d5 DNAFig.5 GelelectrophoresisofPCRamplificationsofthemethanogensDNA 27d,.21～49d,,49～115d,,.127d,,,.3 (1)PTA.136d,COD2950 303kg·(m3·d)-1,3～4d,PTACOD75%～85%,.(2)PTA,.(3).,.136d,.(4)EPS、DNAPCR,.:[1] .[M].:,2003.16-17.[2] .[M].:,2002.1-10.[3] .[D].:,2004.41-44.[4] .[D].:,2006.61-63.[5] .-LAS[D].:,2002.69-70.[6] .SRB[D].:,2004.69-71.[7] .[M].:,1998.509-519.[8] KubaT,FurumaiH,KusudaT.Discussionontheextractionmethodsofextracellularpolymer[J].ProcEnvironEngRes,1992,29:114-116.[9] LowryOH,RosebroughNJ,FarrAL,etal.Proteinmeasurementwiththefolinphenolreagent[J].JBiolChem,1951,193:265-275.[10] RoeJH.Thedeterminationofsugarinbloodandspinalfluidwithanthronereagent[J].BiolChem,1955,212:335-343.[11] RoeJH.Thedeterminationofdextraninbloodandurinewithanthronereagent[J].BiolChem,1954,208:889-896.[12] KageyamaK,KomatsuT,SugaH.RefinedPCRprotocolfordetectionofplantpathogensinsoil[J].JGenPlantPathol,2003,69:153-160.[13] ZhouW,KojiK,LiF,etal.Monitoringofmicrobiologicalwaterqualitybyreal-timePCR[J].EnvironmentalTechnology,2007,28:545-554.[14] MorganJW,ForsterCF,EvisonL,etal.AComparativestudyofthenatureofbiopolymersextractedfromanaerobicandactivatedsludge[J].WaterRes,1990,24(6):743-753.[15] ,,.[J].,2005,28():121-122.[16] JiaXS,FangHP,FurumaiH.Surfacechargeandextracellularpolymerofsludgeintheanaerobicdegradationprocess[J].WatSciTechnol,1996,34(5):309-316.[17] UrbainV,BlockJC,ManemJ.Bioflocculationinactivatedsludgeaanalyticapproach[J].WaterRes,1993,27(5):829-838.[18] FrlundBO,PalmgrenR,