:2002-03-27:(29877008);:(1959-),,,,,.E-mail:lumingma@online.sh.cn马鲁铭,高廷耀,周增炎(, 200092):()(),.:COD,;;COD.:;;:X52 :A :0253-374X(2003)02-0229-04FormationofIntracellularBiopolymerbyAerobicFlocSludgeandFilamentousMicroorganismsMALu-ming,GAOTing-yao,ZHOUZeng-yan(NationalEngineeringResearchCenterforUrbanPollutionControl,TongjiUniversity,Shanghai200092,China)Abstract:Twosequencingbatchreactors(SBRs)andtwocontinuousflowstirred-tankreactors(CFSTR)wereoper-atedtoinvestigatetherelationbetweentheactivatesludgefilamentousbulkingandtheformationofintracellularpoly-mers,whichusedtwokindsofsyntheticwastewatercontainingglucoseoracetaterespectively.ExperimentalresultsshowthatduringtheaerationtheCOD(chemicaloxyendemand)leveldecreasedandtheintracellularpolymerscon-tentsincreasedslowly,withanalmostunchangedCODreductionrate.Therewerefewerintracellularpolymersformedinthebulkingsludgethanintheflocsludge.Moreover,flocsludgehadobviousinitialbothCODrapiddecreaseandin-tracellularpolymersincrease.Keywords:intracellularpolymers;sludgebulking;filamentousmicroorganisms ,,[1],.,(polyhydrox-yalkanoicacidspolyhydroxyalkanoates,PHA),PHA、β-(poly-β-hydroxybutyrate,PHB).,,.1 .、、[1].———(SBR)31220032 JOURNALOFTONGJIUNIVERSITYVol.31No.2 Feb.2003(CFSTR);———.SBR4,、、、(DO).100∶10100∶6,100∶2,100∶1;0.23~1.30d-1;12℃23℃;.CFSTR.100∶12.1.2~6.0d-1,ρ(COD)1000mg·L-1,100~200mg·L-1,,,.,CFSTR.SBRCFSTR,CFSTR,3d.COD90%.,CFSTRCOD80%,,.CFSTR,,,10cm.,,.,,,.,.,,(),,.2 COD2.1 SBR,CFSTR:,0.5h,,400ml,SBR800ml,,.CFSTRCOD,100~200ml·L-1,.ρ(COD)800mg·L-1,.,,.ρ(COD)1.SBR2h,2h,,.45min,,9.15mg·L-1·min-1,6.41mg·g-1·min-1.1 ρ(COD)Fig.1 CODvarietywithaerationtimeinbothSBRandCFSTRfedwithglucose COD,.COD1000mg·L-1,COD73.5%.,4hCOD,5.84mg·L-1·min-1;,,5.26mg·g-1·min-1.COD2,CFSTRSBR.,,.、、.230 31 ,,,,.,COD.,.,:,,,,.2 w()ρ(COD)Fig.2 CODdecreaseandintracellularglycogenincreaseinbothSBRandCFSTRfedwithglucose2.2 PHBCOD3.SBR22dCOD50minCOD;10~30min.50min,COD.3 ρ(COD)Fig.3 CODvarietywithaerationtimeinbothSBRandCFSTRfedwithacetate SBRCFSTR,COD,30min18.5mg·L-1·min-1,6.01mg·g-1·min-1,COD;COD,0.99,3.74mg·L-1·min-1,2.13mg·g-1·min-1,0,..,.,COD,.SBRCODPHB4.PHB60min,1hCOD,PHB;1hCOD,COD,PHB.[2].CFSTRw(PHB).10min,w(PHB)16.1%10.1%,PHB180min,,PHBCODPHB,,,.231 2,: PHB.PHBPHB,.PHB.4 PHBCODFig.4 CODdecreaseandintracellularPHBincreaseinbothSBRandCFSTRfedwithacetate3 (1)CFSTRSBR.;,,,.(2)COD(PHB);COD,,(PHB).(3),.:[1] ,, .PHB[J].,2000,17(9):519-525.[2] MajoneM,MassanissoP,CsrucciA,etal.Influenceofstorageonkineticselactiontocontrolaerobicfilamentousbulking[J].WatSciTechnol,1996,34(5-6):223-232.··S2NS管艮华 (S2NS)(MFC).MFC.《》,MFC.MFC,MFCMFC,.232 31