胞外聚合物EPS对活性污泥沉降性能的影响研究周健

:0253-2468(2004)04-0613-06　　　:X523　　　:AEPS周　健,龙腾锐,苗利利　(,　400045):Ns、(DO)EPS、;EPS.:EPS,DOEPS;EPSSVI;,EPS,SVI.:EPS,Ns、DOEPSSVI.,.:;EPS;;DOEffectofextracellularpolymericsubstances(EPS)onsedimentationofactivatedsludgeZHOUJian,LONGTengrui,MIAOLili　(FacultyofUrbanConstructionandEnvironmentEngineering,ChongqingUniversity,Chongqing　400045)Abstract:Theeffectofloadingrate(Ns)anddissolvedoxygen(DO)oncomposition,contentofextracellularpolymericsubstances(EPS)andsedimentationperformanceofactivatedsludgewerestudied.ResultsshowedthatNssignificantlyaffectedEPSand,andtheeffectofDOonEPSwasnegligible.AsNsreducing,thecontentofEPSandSVIincreasedandtheyweresignificantlycorrelatedeachother.Ontheotherhand,resultsoforthogonalexperimentindicatedthatthefactorcontrollingthecontentofEPSwasNs,andthemodeofNsandDOaffectingEPSandSVIwasdeduced.Inaddition,accordingtoexperimentresults,thecausesofsludgebulkingatlowloadingratecouldbeasfollows:theEPSwasmore,moreoverapartofEPSmightbethenutrimentoffilamentousbacteriaandpromotedfilamentousbacteriaincrease.Keywords:Sedimentationperformanceofsludge;EPS;loadingrate;DO:2003-09-02;:2003-12-16:(59838300);(7279):　(1964—),,,[e-mail](ExtracellularPolymericSubstances),,,[1],EPS,;EPS,EPS50%～90%[2],EPS80%[3],EPS.EPS.,EPS.CEPS,、、,.[4]EPS,EPS、、DNASVI,EPS;SVI;EPS,EPS,Magara[3]24420047　　　　　ACTASCIENTIAECIRCUMSTANTIAEVol.24,No.4July,2004DOI:10.13671/j.hjkxxb.2004.04.009EPS,,.Forster[5]:EPS,,,ZetaSVI,ZataSVI.Chao:SVI100725,Zeta.Barber28:SVIZeta.,EPS,EPS,,.,,,;,EPS.,EPS、,EPS,.1　1.1　,-,15L,,.1.2　1.2.1　EPS　4,,Ns0.3,1,23kg·(kg·d)-1,COD=200～880mg·L-1,BOD5=112～335mg·L-1,SS=103～375mg·L-1,MLSS=2.5g·L-1,DO=2～3mg·L-1,pH=7.34,18℃;HRT90min,70min,50min,30min.SV、SVI、EPS、COD、BOD5SS.EPS、DNA、[6].1.2.2　DOEPS　3,DO0.5,2.24.2mg·L-1,COD=630～815mg·L-1,BOD5=191～312mg·L-1,SS=273～356mg·L-1,Q=300mL·min-1、MLSS=2.5g·L-1,Ns=3kg·(kg·d)-1,HRT=50min,pH=7.42,19℃.SV、SVI、COD、BOD5、SS,EPS、DNA、[6].1.2.3　DO、NsEPS　,22L4(23),NsDO,1,4,EPSSVI,EPS、DNA、[6].1　Table1　LeveloffactorsNs(kg·(kg·d)-1DO(mg·L-1)+13.03.8-10.50.21.2.4　EPS　EPS,EPS.EPS,174mg·L-1EPS,,OUR0.09g·(kg·h)-1;7h,MLSS3.5g·L-1,pH=7.28、18℃.614　　　　　　　　　　240,0.5,1,2,3,5,7h,CODEPSDNA.2　2.1　EPS2　EPS、SVITable2　InfluenceofloadingrateonEPSandSVI(kg·(kg·d)-1)SVI(mL·g-1)EPS(mg·g-1)DNA(mg·g-1)(mg·g-1)(mg·g-1)0.386±223.4±2.58.02±1.003.76±0.5011.6±1.00172±419.6±2.07.06±0.703.58±0.308.92±1.00260±215.8±1.55.54±0.502.47±0.207.79±0.80349±314.5±1.55.21±0.502.39±0.206.92±0.8040d,40d1.EPS2、2、3.12:,0.3　　　　　　　　　1　Fig.1　Influenceofloadingrateonsedimentationperformance2　EPSFig.2　InfluenceofloadingrateoncomponentandcontentofEPS3　SVIEPSFig.3　CorrelatiionanalysisofSVIkg·(kg·d)-13kg·(kg·d)-1,SVI8050,11.9g·L-120.9g·L-1;EPS23.4mg·g-114.5mg·g-1,11.6mg·g-16.92mg·g-1,40%.,EPSDNA、,SVI,SVIEPS,3,:SVIEPS,.,,.:.2.2　DOEPS30d,SVI4.DOEPS3、5.4:,DO0.5mg·L-14.2mg·L-1,SVI41　　　　6154　:EPS4　DOFig.4　InfluenceofDOonsedimentationperformance5　DOEPSFig.5　EffectofDOoncomponentandcontentofEPS3　DOEPS、SVITable3　InfluenceofDOonEPSandSVIDO(mg·L-1)SVI(mL·g-1)EPS(mg·g-1)DNA(mg·g-1)(mg·g-1)(mg·g-1)0.542±116.1±1.65.28±0.402.05±0.208.73±1.002.247±217.9±1.96.28±0.502.66±0.408.94±1.004.251±219.4±2.06.98±0.53.11±0.509.33±1.0052,SVI,60,;,3:,EPS;DO,EPSDNA、.2.3　DO、NsEPS4.4　、Table4　Thetableofexperimentresult,orthogonalregressioncalculatingandvarianceanalysisX0X1(Ns)X2(DO)X1X2/(mg·g-1)DNAYi1(EPS)DNAYi2(EPS)Yi=Yi1+Yi2Yii=︱Yi1-Yi2︱111116.983.099.3319.406.223.7810.3020.3039.700.90211-1-15.282.058.7316.065.983.0910.0019.0735.133.0131-11-111.803.9013.9029.6010.23.6815.1028.9858.580.6241-1-119.702.4513.3025.459.013.0912.6024.7050.150.75Bj=∑XY183.56-33.9013.00-3.86bj=Bj(4×2)22.94-4.241.63-0.48Qj=bjBj143.7421.131.85F=Qj(Sf)106.4715.651.37(α=0.01)X0X1(Ns)X2(DO)X1X2(SVI)Yi1(SVI)Yi2(SVI)Yi=Yi1+Yi2Yii=︱Yi1-Yi2︱1111154581124211-1-14852100631-11-19296188441-1-1175771522Bj=∑XY532-12848-24bj=Bj(4×2)69-166-3Qj=bjBj204828872F=Qj(Sf)227.55328(α=0.01)　　:S=0.5×∑(Yi1-Yi2)2,f=N×(M-1)=4×(2-1)=4;EPS=22.94-4.24X1+1.63X2,X1=(NS-1.75)1.25,X2=(DO-2)1.8,EPS=27.07-3.39Ns+0.9DO;,SVI=69-16X1+6X2,X1=(NS-1.75)1.25,X2=(DO-2)1.8,SVI=84.74-12.8Ns+3.33DO616　　　　　　　　　　24　　,:EPS=27.07-3.39Ns+0.9DO(1)SVI=84.74-12.8Ns+3.33DO(2)　　,(1)、(2);SVI、EPSNs,DO,,EPS,DO.Ns,EPS,SVI.,ABA、DO;,EPSSVI,.2.4　EPS67,EPS,1h,CODEPS、DNA,,,EPS,EPS;1～2h,COD、DNA,EPS,EPS,COD、DNA;2h,COD、DNA,EPS,.,EPS,;EPS[7],66.5%,EPS34.9%,EPS,.,,,,,.,:,,EPS;,EPS,.6　EPSDNAFig.6　ChangecurveofpolysaccharideandDNAinEPSduringbiodegradation7　EPSCODFig.7　ChangecurveofdissolvableCODinEPSduringbiodegradation　　,,,;EPS,,;,,6174　:EPS;,.,,,,.,μmaxKs[8],;EPS,EPS,EPSDNA(2);2,EPS、DNA,EPS、(6),.,,,EPS,.3　1)EPSSVI,SVI=4.12EPS-21.658;EPS,,EPS,SVI;DO,EPS,SVI.2):EPS,DO,:EPS=27.07-3.39Ns+0.9DO,SVI=84.74-12.8Ns+3.33DO.3)EPS,,EPS,.:[1]　MelanieJBrown,JNLester.Metalremovalinactivatedsludgetheroleofbacterialextracellularpolymers[J].WatRes,1979,13(1):817—837[2]　UrbainetalV.Bioflocculationinactivedsludge:ananalysticapproach[J].WatRes,1993,27(5):829—838[3]　MagaraY,NumbuS,UtosawaK.Biochemicalandphysicalpropertiesofanactivedsludgeonsettlingcharacteristics[J].WatRes.1986,10(1):71