胞外聚合物对浸没式膜生物反应器膜过滤性能的影响王雪梅

,　,,.-[J].,2005,25(12):1602-1607WANGXuemei,LIUYan,HUAZhihao,etal.EffectofextracellularpolymericsubstancesinSMBRonmembranefiltration[J].ActaScientiaeCircumstantiae,2005,25(12):1602-1607-王雪梅,刘　燕＊,华志浩,郭国良复旦大学环境科学与工程系,上海　200433:2005-04-12　　　:2005-08-28　　　:2005-09-04:-(SMBR),2(EPS)、、.,EPSEPS,EPS(EPSP)(EPSC).EPS,EPSEPS.EPS,EPS.EPS.SMBR、53%、59%.2,.:-;;;;:0253-2468(2005)12-1602-06　　:X703　　:AEffectofextracellularpolymericsubstancesinSMBRonmembranefiltrationWANGXuemei,LIUYan＊,HUAZhihao,GUOGuoliangDepartmentofEnvironmentalScienceandEngineering,FudanUniversity,Shanghai200433Received12April2005;　receivedinrevisedform28August2005;　accepted4September2005Abstract:Thispaperinvestigatedtheamountandcompositionofextracellularpolymericsubstances(EPS)inactivatedsludgefrommixedliquoraswellasthoseinsludgesfromahydrophilicandahydrophobicmembrane,respectively,inaSubmergedmembranebioreactor(SMBR)treatingriverwaterinShanghai,andanalyzedthecharacteristicsofthesesludges.TheeffectofEPSandsludgehydrophobicityonfiltrationofmembranewasstudied.TheexperimentresultsshowedthattotalamountofEPSinsludgefrommixedliquorwasmorethanthosefrommembranesurface,andtheratioofEPSPEPSCrankedmixedliquorhydrophilicmembranehydrophobicmembrane.ThequantityandcomponentsofSolubleEPSandBoundEPSweredifferentamongthreekindsofsludges.ThequantityofSolubleEPSfromhydrophobicmembranewasmorethanthatfromhydrophilicmembrane.EPSinsludgefrommixedliquorwaslooserthanthosefromthemembranesurfaces.EPSinsludgefromhydrophilicandhydrophobicmembranesurfacewastightlyadheredtocellsurfaces.SolubleEPSgreatlyaffectedmembranefiltration.Relativehydrophobicityofsludgefromhydrophilicandhydrophobicmembranesurfacewas53%and59%respectively.Comparisonoffluxrevealedthathydrophobicmembranefoulingwasmoreseriousthanhydrophilicone,andtheabilityofanti-foulingofthelatterwashigherthanthatoftheformer.Keywords:submergedmembranebioreactor(SMBR);extracellularpolymericsubstances(EPS);membranefouling;hydrophilicmembrane;hydrophobicmembrane:(No.20377010):(1979—),,,E-mail:[e-mail],Tel:[phone];＊()Foundationitem:NationalNaturalScienceFoundationofChina(No.20377010)Biography:WANGXuemei(1979—),female,mastercandidate,E-mail:[e-mail],Tel:[phone];＊Correspondingauthor　　-(Submergedmembranebioreactor,SMBR),(,2002;Leeetal.,2003).,SMBR(Changetal.,1998;Leeetal.,2003).SMBR,.,(Extracellularpolymericsubstances,EPS).EPS,EPS(Nagaokaetal.,1996;Changetal.,1998;Nagaokaetal.,1998;Choetal.,2002;Rosenbergeretal.,2002;Leeetal.,2003;Gao.etal.,2004).2512200512　　　　　ActaScientiaeCircumstantiaeVol.25,No.12Dec.,2005DOI:10.13671/j.hjkxxb.2005.12.006(Changetal.,1998;Rosenbergeretal.,2002;Leeetal.,2003;Gao.etal.,2004)EPS,EPS、.Nagaoka(1996,1998)SMBREPS.Gao(2004)EPS,EPS.Cho(2002),EPS.EPS,EPS2:EPS(SolubleEPS,EPSS)EPS(BoundEPS,EPSB)(Murthyetal.,1998;Wingengderetal.,1999;Rosenbergeretal.,2002).EPSS,、、、、、EDTA(Liuetal.,2003)EPSEPSB.Rosenberger(2002)EPSB,EPSS,EPSSEPSB.2EPS,EPS,.(solublemicrobialproducts,SMP),SMBR.SMPEPS(Laspidouetal.,2002),、、、DNA.SMP;EPS,.,SMP(Fangetal.,1998;Huangetal.,2002),EPS(Nagaokaetal.,1996;Nagaokaetal.,1998;Murthyetal.,1998;Wingengderetal.,1999;Liuetal.,2003).SMPSMBR,,EPS,SMP.SMBR,SMBREPSSEPSB、,SMP,EPSSMBR.1　(Experiments)1.1　试验材料,.1.2　试验装置SMBR,,(PVDF,).,50°,PM;,90°,PM.20.06m20.22μm,.(250mm×160mm×600mm),8L..1.3　试验方法2PVDFPMPM,EPS、SMPEPS,2,.,20℃.10d1,,COD、BOD5、NH+4-N、NO-2-N、NO-3-N、、、pH、.,SSVSS,EPS.SMP.EPSSMP、、DNA、,.,.1.4　分析测定方法1.4.1　水质参数　(《》,1989):NH+4-N,-;NO-3-N,;NO-2-N,N-(1-)-;CODCr,;BOD5,;TP,;pH,pH;,;,;,.1.4.2　EPS和SMP的提取及分离方法　EPSS(Murthyetal.,1998).(5mL)7mL,5mL,12000r·min-120min,0.22μm,EPS.160312:-EPSB(Morganetal.,1990;Liuetal.,2001;,2004).(5mL)7mL,5mL,5000r·min-15min,,.5mL,80℃30min,12000r·min-120min,0.22μm,EPSB(Murthyetal.,1998).0.22μmSMP(Fangetal.,1998;Huangetal.,2002).1.4.3　EPS组分的测定方法　EPS、、、DNA(Jahnetal.,1998;Wingengderetal.,1999;Liuetal.,2003).EPSVSS.(Brownetal.,1980;Frφlundetal.,1995;Zhangetal.,1999),;Lowry(Frφlundetal.,1995),;DNA(Danielsetal.,1994;Liuetal.,2002),.1.4.4　污泥特性　BATH(BacterialAdherencetoHydrocarbon)(Rosenberg.,1980;Rosenbergetal.,1984);(,1999);SS、VSS(《》,1989);.2　(Results)2.1　反应器运行及水质情况150d,5.6mg·L-1,pH7.5,SS1.2～1.8g·L-1,VSS0.7～1.2g·L-1.,60d.,,1.1,2.1　Table1　QualityofinfluentandeffluentinSMBRpHCODCr(mg·L-1)BOD5(mg·L-1)TP(mg·L-1)NH+4-N(mg·L-1)NO-3-N(mg·L-1)NO-2-N(mg·L-1)(mg·L-1)82550～10012～142.310～250.50.2～2440～500PM7.51015～501～21.50.510～200.5130～160PM7.51015～501～21.70.510～200.5130～1602.2　EPS的量和组分变化5.2.2.1　混合液污泥和2种膜上污泥的EPSB　1a、2EPSB.,EPSB,2EPSB.EPSB,(EPSP)(EPSC).1　2EPS(a.EPSB;b.EPSS;c.)Fig.1　QuantityandcomponentsofEPSfromsludgeinmixedliquorandonmembranes(a.EPSB;b.EPSS;c.attachedSubstancesbylowspeedcentrifugation)1604　　　　　　　　　　25　　1b1cEPSS.,EPSS,2,.2,EPSSDNA,DNA,.2.2.2　SMP、进出水中的大分子物质的量及成分　2,、DNA,.DNA,,4～5.,SMPDNA,.2　SMPTable2　Quantityandcomponentsofmicro-molecularcompoundsininfluentandeffluentandSMPmg·L-1DNA4.93.6——PM23.54.9——PM19.95.0——SMP22.62.4——2.3　污泥特性2:PM53%,PM59%.,,PMPMEPSPEPSC2.73.5.EPS,,.、、(Leeetal.,2003).,SMBR1.5×10-3Pa·s,1.2×10-3Pa·s,.(1.65～2.83)×1013cm·g-1(,,1999),2.8