胞外聚合物与溶解性微生物产物的关系王金翠

31121.0500612.300072、。0~19hboundEPSBAPSMP。19~43hSMPboundEPSUAPSMPboundEPS。43~162hSMP、、。boundEPSSMP-。162~210hboundEPS。SMPSMP。X172A1003-6504(2008)11-0018-03RelationshipbetweenExtracellularPolymericSubstanceandSolubleMicrobialProductsWANGJin-cui1SUNBao-sheng21.InstituteofHydrogeologyandEnvironmentalGeologyCAGSShijiazhuang050061China2.CollegeofEnvironmentalScienceandEngineeringTianjinUniversityTianjin300072ChinaAbstractThroughexperimentofactivatedsludgedecayprocess,itwasfoundthatthereexistedrelationshipsofconversionandadsorption-desorptionbetweenextracellularpolymericsubstances(EPS)andsolublemicrobialproducts(SMP).Inthebeginningofdecayprocess(0~19h),boundEPSwerehydrolyzedtobiomassassociatedproducts(BAP),andthecelllysisproductsalsoconstitutedpartofSMP.InthephaseofSMPbiodegradation(19~43h),partofSMP,beingbiodegradable,wereutilizedbyactivebiomassasrecycledelectrondonors.BoundEPSandutilizationassociatedproducts(UAP)wereproduced.HighmolecularweightfractionsofSMPwereadsorbedbyboundEPS,andthereexistedcelllysisfaintly.Intheexcretingandautolyzingphase(43~162h),decayprocessenhancedanddehydrogenaseactivityofmicroorganismslessenednearlytonone.Celllysisandautolysisoccurred.Thereexisteddesorption-adsorptionbetweenboundEPSandhighMWfractionsofSMP.Inthephaseofbiodegradingandautolyzingprocess(162~210h),dominatingprocesswasboundEPShydrolyzingtoBAPandbacteriaautolysis.SMPwerenotbiodegradableeasily,andtheyhadsomeeffectoftoxicityonmicroorganism,restrainingthedehydrogenaseactivityofbiomass.Whileinthepresenceoftoxicmaterials,moreSMPwerereleasedbymicroorganisms.Keywordsextracellularpolymericsubstance(EPS);solublemicrobialproduct(SMP);hydrolyzing;autolysis;adsorptionEnvironmentalScience&Technology3111200811Vol.31No.11Nov.20082008-0[e-mail]。solublemicrobialproductsSMPextracellularpolymericsub－stancesEPS。SMPsub－strateutilizationassociatedproductsUAPbiomassassociatedproductsBAP。EPSboundEPSsolubleEPS[1]。SMPEPSLaspidou[2]SMPsolubleEPSboundEPSUAPboundEPSBAPUAPBAP。Aquino[3]boundEPSBAPSMPBAP。、DOI:10.19672/j.cnki.1003-6504.2008.11.00511、[4]。SMPEPS。13800mL3380mg/L。130r/min30℃MLSS、EPS、SMP、DNADHA。1。MLSS[5]SMP0.45μmTOC[6]EPS、、TOC[7]DNA[8]DHATTC-[9-11]。2210h4462mg/L210h3380mg/L2.82×10-2d-1。2.1EPS、SMPDNA2EPSSMPDNA19~210hEPS。0~19h[12]boundEPSBAPDNA19~43hSMPboundEPSUAPEPSSMPEPS43~67hSMPboundEPSBAPEPS67~162hSMP。boundEPSSMP。67~90hEPSEPS90hEPS162~186hEPSSMP186~210hSMPEPSSMP。2.2323.90μgTF/mgMLSS·h90h1.27μgTF/mgMLSS·h。SMPSMPSMP[11-13]。33110~19hboundEPSBAPSMP。2SMP19~43hSMPDNAboundEPSUAPSMPboundEPS。。3SMP、43~162h。boundEPS。4SMP、162~210hboundEPSBAP。。5SMPSMP。[][1].[J].200733170-174.WangJin-cuiSunBao-sheng.Progressofstudiesonmi－crobialsecondarymetabolitesinbiologicalwastewatertreat－ment[J].WaterandWastewaterEngineering200733170-174.inChinese[2]LaspidouCSRittmannBE.Aunifiedtheoryforextracellu－larpolymericsubstancessolublemicrobialproductsandactiveandinertbiomass[J].WaterResearch2002362711-2720.[3]AquinoSFStuckeyDC.Solublemicrobialproductsforma－tioninanaerobicchemostatsinthepresenceoftoxiccom－pounds[J].WaterResearch200438255-266.[4].-EPS[J].20063108-110.NiBing-jieXuDe-qianLiuShao-gen.ExtracellularpolymericsubstanceEPSanditsinfluenceonpropertiesofactivatedsludge[J].EnvironmentalScience&Technology20063108-110.inChinese[5].GB/T11901-1989,[S].1989-11-03.StateTechicalSupervisonBureauofthePeople'sRepublicofChina.GB/T11901-1989,statestandardofChina[S].BeijingStandardsPressofChina1989-11-03.inChinese[6]BoeroVJEckenfelderJrWWBowersAR.Molecularweightdistributionofsolublemicrobialproductsinbiologi－calsystems[J].WaterScienceandTechnology1996345-6241-248.[7]XiaoqiZhangPaulLBKinkleB.Compositionofextrac－tionmethodsforquantifyingextracellularpolymersinbiofilms[J].WaterScienceandTechnology199939211-218.[8].[M].2005:238-244.YuJian-yingJiangYuWangShan-li.TechniqueofEx－perimentationforBiochemistry[M].BeijingChemicalIn－dustryPress2005:238-244.inChinese[9].[M].2001:221-229.ChenShi-genZhouRun-qi.Enzymology[M].ShanghaiFudanUniversityPress2001:221-229.inChinese[10].TTC-[J].199511416-19.YinJunZhouChun-sheng.NormaltemperatureextractionmethodfortheexaminationofTTC-Dehydro-Genaseac－tivityanditsapplication[J].ChinaWaterandWastewater199511416-19.inChinese[11].[M].1983:144-171.ZhengYuan-jingShenGuang-fanWuYang-shan.Wastew－aterTreatmentbyBiofilms[M].BeijingChinaArchitectureIndustryPress1983:144-171.inChinese[12]KuoWC.ProductionofSolubleMicrobialChelatorsandTheirImpactonAnaerobicTreatment[D].UniversityofIowaIowaCity1993.[13]BarkerDuncanJStuckeyDC.Areviewofsolublemicro－bialproductsSMPinwastewatertreatmentsystems[J].WaterResearch199933143063-3082.