北黄海沉积物水界面反硝化速率及影响因素研究白洁

———白　洁1,2,王晓东1,2,李佳霖1,2,宋　亮1,2,于江华1,2,魏　皓3,张桂玲4(1.;2.;3.;4.,266100)　:　,,2.5～5.8μmol/m2·h,4.85μmol/m2·h。,。。:　;;N2O;:　X832　　　　　:　A　　　　　:　1672-5174(2007)04-653-04　　,、、,,40.6Tg/a[1],1[2]。[1-5],[2]、[6]。,[8]。,-,。,,[3]。,[3]。(C2H2)[7]。C2H2N2ON2,Srensen,(AIT)[6]。、、,NO3-,-NO3-,,,、-[7]。,,。1　1.11.1.1站位布设　　20066,“2”,120°59′E～123°E,37°27′N～38°57′N8,1。1　Fig.1　Thesamplingstations1.1.2样品采集　　15cm,。、。1.2:(2006CB400602);(200302);(2004040-1):2007-04-04;:2007-06-05:　(1962-),,,,。E-mail:[e-mail]　37　4　20077　PERIODICALOFOCEANUNIVERSITYOFCHINA37(4):653～656July,2007DOI:10.16441/j.cnki.hdxb.2007.04.025,14cm、45cm。10cm,,,。,,。,,。,,,。1.3,HP5890Ⅱ。-AA3。DOYSI6600。、、pHPCD6500。1.4N2O,[2]:F=H×10-3×(■c/■t)。:F[μmol/(m2·h)],H(m),■c/■tN2O[μmol/(L·h)]。2　2.13.2～7.5μmol/(m2·h),4.85μmol/(m2·h)。2a。,———。2a,,,,。2.2、、[9]。DO、、、pH1。2　(a)(b)Fig.2　Thedistributionsofdenitrificationrate(a)andDO(b)intheNorthYellowSea1　Table1　ThecorrelationbetweenenvironmentalfactorsandthedenitrificationrateintheNorthYellowSeaEnvironmentalfactors/℃Temperature/g·L-1SalinityDO/mg·L-1NH4+/μmol·L-1NO3-/μmol·L-1pHDatarange7.2～1631.3～32.34.5～5.743.9～69.43.1～6.27.8～8.1Correlationcoefficint0.35-0.16-0.860.260.12-0.32P0.3920.7140.0060.6750.7750.4382.2.1反硝化速率与溶解氧的关系　　1[3],,,,、(N2O),[3]。654　　　　　　　2007(2),,,,。,(r=-0.86,P0.01),。Seitzinger,,。,;DO,。,,,[10]。,。2.2.2反硝化速率与温度的关系　　,,,,[10]。1,0.35(P0.05),,(7～16℃),,,。2.2.3反硝化速率与盐度、pH值的关系　　RysgaardRamdersFjood,010,50%;[10]。(r0.16,P0.05)。31.3～32.3,,,。pH7.0～8.0,pH9.5,pH6.0[10]。pH(r-0.32,P0.05),pH,,pH。2.2.4反硝化速率与营养盐含量的关系　　NO3-,MorrisMcMahon,NO3--N[10],,NO3--N71μmol/L,NO3--N,。NO3--N0.12(P0.05),,。2.32。,[4][2],2.0～7.0mg/L、2.0～5.0mg/L,(24～30℃),(56～180μmol/L),(0～16);,;Southernnorthsea,GulfofFinlandNorthernBalticProper,。(38m)、(71000km2),[8],,。2　Table2　TheratesofdenitrificationindifferentcoastalareasSpotDenitificationrateμmol/m2·hMeasureRanderFjord,Denmark[11]　　60～420AITKysingFjord,Denmark[11]　　9～3301AITDelawareInlet,America[11]　　60～119AITGreatSouthBay,USA[11]76～380AITNorsmindeFjord,Denmark[11]827～4170AITNewportRiverEstuary,America[11]42～125AITTomalesBaymudflat,California[11]30～298AITTorridgeRivermarsh,UK[12]24～589AITChesapeakeBay,Zmarina[12]670～2089AITGreatOuseEstuary,UK[12]21～95AITTexel,WaddenSea,Germany[12]9～551AITSouthernNorthSea[12]6～9AITNarragansettBay,USA[12]149～1949N2GuadalupeEstuary,Mexico[12]45～345N2BostonHarbour[13]30～1226N2MassachusettsBay[13]430～38115NPatuxentRiverEstuary,Maryland[13]771～89015NTokyoBay,Japan[13]161～33015NClonePointsaltmarsh,UK[13]39～13115NAarhusBay,Denmark[14]119～21115NSouthernNorthSea[14]9～1215NArcachonBay,France[14]0～17615NCloneEstuary,UK[14]3～45815NGulfofFinland[14]3～2715NNorsmindeFjord,Denmark[14]42～66715NNorthernBalticProper[14]0～12.515N[2]21.91～35.87AIT[6]30～800AIT()3.2～7.5AIT:AIT—;N2—N2;15N—15N。Note:AIT-acetyleneinhibitiontechnique;N2-N2fluxtechnique;15N-15Ntracetechnique6554,:———3　,3.2～7.5μmol/(m2·h),,。,。,,。:[1]　NedwellDB,JickelsTD,TrimmerM,etal.Nutrientsinestuaries[M].//NedwelDB,RafaelliD,Eds.Advancesinecologicalre-search:Estuaries.NewYork:AcademicPress,1999:43-92.[2]　,,,.N2O[J].,2006,35(3):271-279.[3]　TanitaSirivedhin,KimberlyAGray.Factorsaffectingdenitrificationratesinexperimentalwetlands:Fieldandlaboratorystudies[J].Eco-logicalEngineering,2006:167-181.[4]　JaapVanRijn,YossiTal,HaroldJSchreier.Denitrificationincon-centrationrecirculatingsystems:Theoryandapplications[J].Aqua-culturalEngineering,2006,34:364-376.[5]　AndrewELaursen,SybilPSeitzinger.TheroleofdenitrificationinnitrogenremovalandcarbonmineralizationinMid-AtlanticBightsedi-ments[J].ContinentalShelfResearch,2002,22:1397-1416.[6]　,,,.DIN[J].,2005,25(5):686-692.[7]　,.[J].,2003,3:27-28.[8]　,,,.[J].,2005,79(1):114-123.[9]　OgiluieB,NedwellDB,NarrisonRM,etal.Highnitrate,muddyestuariesasnitrogensinks:Thenitrogenbudgetoftherivercolnees-tuary(UK)[J].MarineEcologyProgressSeries,1997,150:217-228.[10]　,.[J].,2003,(4):41-42.[11]　HerbertRA.Nitrogencyclingincoastalmarineecosystems[J].FFMSMicrobiologyReviews,1999,23:563-590.[12]　HowarthRW,BillenC,SwaneyD,etal.RegionalnitrogenbudgetsandriverineN&PfluxesforthedrainagestotheNorthAtlanticO-cean:Naturalandhumaninfluences[J].Biogeochemistry,1996,35:75-139.[13]　CaracoNF,ColeJJ.Humanimpactonnitrateexport:ananalysisusingmajorworldrivers[J].Ambio,1999,28(2):167-170.[14]　BoyntonWR,GarberJH,SummersR,etal.Inputs,transforma-tionsandtransportofnitrogenandphosphorusinChesapeakeBayandselectedtributaries[J].Eestuaries,1995,18:285-314.AStudyoftheDenitrificationRateandItsInfluenceFactorsattheSediment-WaterInterfaceinSummerintheNorthYellowSeaBAIJie1,2,WANGXiao-Dong1,2,LIJia-Lin1,2,SONGLiang1,2,YUJiang-Hua1,2,WEIHao3,ZHANGGui-Ling4(OceanUniversityofChina1.KeyLabofMarineEnvironmentalScienceandEcology,MinistryofEducation;2.CollegeofEnvi-ronmentalScienceandEngineering;3.CollegeofMarineMeteorology;4.CollegeofChemistryandEngineering,Qingdao266100,China)Abstract:　ThedistributionofdenitrificationrateinsummerintheNorthYellowSeawasmeasuredusingtheacetyleneinhibitiontechnique.Thedenitrificationratewasfoundofbefrom3.2to7.5μmol/m2·h,theaver-agebeing4