苯酚对厌氧氨氧化污泥脱氮效能长短期影响

3610201510ENVIＲONMENTALSCIENCEVol．36No．10Oct．2015121212*1212121．2150112．215011ANAMMOXANAMMOX．．600mg·L－1NH+4-N6%TN10%．100mg·L－1NH+4-N99%ANAMMOX．400mg·L－1NH+4-N23.59%TN50.3%ANAMMOX．ANAMMOX．1000mg·L－1．ANAMMOXIC5071.57mg·L－1．18dNH+4-NρNH+4-N/ρNO－2-N/ρNO－3-N1∶0.86∶0.2．．X703.5TQ028.8A0250-3301201510-3771-07DOI10．13227/j．hjkx．2015．10．0282015-04-132015-05-145140838751478284CXLX14_1289SKCX14_0281990～[e-mail]*[e-mail]ceofPhenolonNitrogenＲemovalEfficiencyofANAMMOXSludgeYANGPeng-bing12LIXiang12HUANGYong12*ZHULiang12CUIJian-hong12XUShan-shan121．SchoolofEnvironmentalScienceandEngineeringSuzhouUniversityofScienceandTechnologySuzhou215011China2．InstituteofEnvironmentalBiotechnologySuzhouUniversityofScienceandTechnologySuzhou215011ChinaAbstractTheeffectsofphenolconcentrationonnitrogenremovalefficiencyinANAMMOXsludgewerestudiedinshortorlongterm．Thenitrogenremovalratedecreasedrapidlywiththeincreaseofconcentrationofphenolinshort-term．Whenthephenolconcentrationwasabove600mg·L－1theremovalrateofNH+4-Nwaslowerthan6%andtheremovalrateofTNwasonlyabout10%．Thelong-termexperimentalresultsshowedwhenthephenolconcentrationwasbelow100mg·L－1theremovalrateofNH+4-Ncanreach99%whichindicatedthatANAMMOXbacteriahaveaprocessofadaptationtothelowconcentrationofphenol．Howeverwhenthephenolconcentrationwasabove400mg·L－1theremovalrateofNH+4-NandTNwasonly23.59%and50.3%theactivityinsludgewassignificantlyinhibitedwhichwassimilarlytotheshort-termandtheactivityofdenitrifyingbacteriawassignificantlyhigherthanANAMMOXbacteria．Asanorganiccarbonsourcephenolcanactivateddenitrificationreactionandfinallyplaysadominantroleinthesystem．Howeverthehighconcentrationofphenol1000mg·L－1alsohasaninhibitoryeffectondenitrificationbacteria．HalfinhibitoryconcentrationIC50ofphenoltoANAMMOXactivitywas71.57mg·L－1byfitting．After18days'recoverythestoichiometricratiosofnitrogenwerechangedalthoughtheNH+4-Nwasbasiclyremoved．TheratioofremovedNH+4-NreomvedNO－2-NandproducedNO－3-Nwas1∶0.86∶0.2．Ourresultsshowedthattheconcentrationofphenolshouldbecontrolledwithinareasonablerangesothatthereactorcanachievesimultaneousremovalofphenolandnitrogen．KeywordsANAMMOXphenoldenitrificationnitrogenremovalefficiencyeffectANAMMOX、1～345、6、78、9、10．、、C/N、ANAMMOX．11～13ANAMMOX．36ANAMMOX1415ANAMMOX．ANAMMOXANAMMOX．11.1100mL250mL．33℃125r·min－1pH7.8～8.20.25mol·L－1HCl．30min．1.24aANAMMOXMLVSS/MLSS0.42．NH4ClNH+4-N100mg·L－1、NaNO2NO－2-N130mg·L－1、NaHCO32000mg·L－1、KH2PO427mg·L－1、CaCl2·2H2O136mg·L－1、MgSO4·7H2O200mg·L－1．ⅠEDTA5000mg·L－1FeSO45000mg·L－1ⅡEDTA5000mg·L－1ZnSO4·7H2O430mg·L－1CoCl2·6H2O240mg·L－1MnCl2·4H2O990mg·L－1NaMoO4·2H2O220mg·L－1NiCl2·6H2O190mg·L－1NaSeO4·10H2O210mg·L－1H3BO414mg·L－1．Ⅰ1mL·L－1Ⅱ1.25mL·L－1．50g·L－1．1.31.3.1ANAMMOX182g18100mL．32℃15h1844105%．100、100、200、300、400、500、600、700、8001000mg·L－1．15h、pH、NH+4-N、NO－2-N、NO－3-NANAMMOX．2．1.3.22g250mL．1dANAMMOX．1.416．NH+4-NNO－2-NN-1--NO－3-NpHpH2114-MLSSMLVSSTNNH+4-N、NO－2-NNO－3-NTN．22.1NH+4-N100mg·L－1、NO－2-N130mg·L－115h1NH+4-N、NO－2-N．NH+4-N、NO－2-N22.67mg·L－1、62.60mg·L－1NH+4-N78.1%TN56.7%．100mg·L－1NH+4-N、NO－2-N65.7mg·L－1、93.11mg·L－1NH+4-N34.3%TN24.8%ANAMMOX．600mg·L－1ANAMMOXNH+4-N、NO－2-N90mg·L－1、118mg·L－1NH+4-N、TN6%、10%．TNNH+4-N277310TNANAMMOX1TN．1Fig．1VariationofnitrogenremovalefficiencyonANAMMOXwithphenolconcentrationinshort-time17CuANAMMOX3、．ANAMMOXNH+4-NNO－2-N1．18．19ANAMMOX0.04mmol·L－10.06mmol·L－1NH+4-N0.08mmol·L－1NH+4-N．ANAMMOX．0.2%1%．100～1000mg·L－10.01%～0.1%．20100mg·L－1400mg·L－1．100mg·L－1NH+4-NTN50%ANAMMOX600mg·L－1ANAMMOX．Yang21CuANAMMOX75mg·L－148.7%．ANAMMOX．2.2NH+4-N100mg·L－1、NO－2-N130mg·L－12．0～28d60mg·L－1100mg·L－1ANAMMOXNH+4-N、NO－2-N99%NO－3-N．ANAMMOXANAMMOX．100mg·L－1NH+4-N、TN．NH+4-N．1000mg·L－1NH+4-N10%TN27%ANAMMOX．NO－2-NNO－3-NNH+4-N200mg·L－1NO－2-NNO－3-N0mg·L－1NO－2-N100%NO－3-N．400mg·L－1NO－2-N100%NO－3-N．Zhu22．110d600mg·L－1430mg·L－1HＲT20.75h95.5%96.75%．．NO－2-NNO－3-N377336．1000mg·L－1NO－2-N46.5%NO－3-N．95d0mg·L－1ANAMMOX．18dNH+4-N99%NO－2-N64%～65%NH+4-NNO－2-N．ANAMMOX．2Fig．2EffectofnitrogenremovalefficiencyonANAMMOXwithphenolconcentrationinlong-time3Fig．3MorphologyofANAMMOXofactivatedsludgeinhibitionbyphenol2.3ANAMMOX3a．Strous23ANAMMOX．46d3b14．94dANAMMOX3c．95d18d3d．NH+4-NANAMMOX11d．NH+4-NANAMMOX．ρNO－2-N/ρNH+4-N/ρNO－3-N1∶0.86∶0.21∶1.32∶0.26．ANAMMOXNO－2-NANAMMOXNO－2-NNH+4-N．．4773102.4200mg·L－1NH+4-NANAMMOXNO－2-N100%NO－2-N．Toh24ANAMMOXANAMMOX25ANAMMOXANAMMOX．ANAMMOXNO－2-N．NO－2-N4aNO－2-NNO－2-N．500mg·L－1NO－2-NNO－3-N．26ANAMMOXANAMMOX0.2mmol·L－118.82mg·L－1NH+4-N、NO－2-NNO－3-N1∶1.52∶0.11NO－2-NNO－3-N．NH+4-N、NO－2-NNO－3-N4b100mg·L－1ρNO－2-N/ρNH+4-N1.265ρNO－3-N/ρNH+4-N0.18NH+4-NYang21．200mg·L－1ρNO－2-N/ρNH+4-N1.3214.66．TNANAMMOX．．ANAMMOX1∶1.32∶0.264Fig．4PhenolinduceddenitrificationreactioninthesystemANAMMOXTN4c．．NH+4-N100mg·L－1NO－2-N130mg·L－1V250mLTNTN=100+130mg·L－1·0.25LNH+4-NANAMMOXNH+4-NANAMMOX．ANAMMOX1mgNH+4-N1.32mgNO－2-N0.26mgNO－3-N．NH+4-NNH+4-NNH+4-N=NH+4-N·100mg·L－1·5773360.25LANAMMOXTNTN=NH+4-N·100·1+1.32－0.26mg·L－1·0.25LANAMMOXTNAA=NH+4-N·100·1+1.32－0.26mg·L－1·0.25L·100+130mg·L－1·0.25L－1TNBB=TN－ANAMMOX≤300mg·L－1ANAMMOXTN80%TN1