不同pH条件下酵母菌处理高含油废水的研究

79Vol.7,No.920069TechniquesandEquipmentforEnvironmentalPollutionControlSep.2006pH孙玉红1,2　吕文洲1,3　高　静2　杨　敏1*(1.,100085;2.,300130;3.,315211)　　,(SBR)pH。pH,pH4～5、COD/,pH5。100dSBR,pH=5、。　　　　pH　X172　　　A　　　1008-9241(2006)09-0094-05Treatmentofhigh-strengthoil-containingwastewaterbymixedyeastculturesunderdifferentpHSunYuhong1,2　LuWenzhou1,3　GaoJing2　YangMin1(1.SKLEAC,ResearchCenterforEco-EnvironmentalSciences,ChineseAcademyofSciences,Beijing100085;2.SchoolofChemicalEngineeringandTechnology,HebeiUniversityofTechnology,Tianjin,300130;3.CollegeofArchitectural,CivilEngineeringandEnvironment,NingboUniversity,Ningbo315211)Abstract　Wastewatertreatmentperformancewasinvestigatedinsixsequencingbatchbioreactors(SBR)whichwereoperatedunderdifferentpHforthetreatmentofhigh-strengthoil-containingwastewaterusingmixedyeastisolates.BycomparisonwithneutraloralkalipHconditions,theconditionofpH4～5demonstratedbetterperformanceintermsoffoam-formingcontrol,yeastgrowthandCODandoilremovals,etc.,andpH5wastheop-timalpHforthetreatmen.tThe100-daycontinuouswastewatertreatmentusingalarge-scaleSBRindicatedthatthesystemcouldbeoperatedsteadilyandefficientlyatpH5.Keywords　yeast;oil-containingwastewater;biologicaltreatment;pHeffects:(20521140076);(50238050):2005-12-19;:2006-03-10:(1980～),,,。E-mail:[e-mail]*,E-mail:[e-mail]　　,[1～4]。,,,[5]。,pH。pH,,pH。,pH。,pH,。,pH[3,6,7]。、、COD/、、pH、Zata,pHSBR,pH,pHSBR。1　1.1　5:9:pH(Candidautilis;CU);(Candidaboidinii;CBR);(Candidatropicalis;CT);(Rhodotorularubra;RRL);(Trichosporoncutaneum;TC)3:Candidalipolytica、CandidaintermediaCandidapseudolambica。YPD,28℃,175r/min24h。,。:COD390～415.2g/L,TN170～190g/L,BOD174.5～183.3g/L,TP3.1～3.3g/L,101.6～129.5g/L,pH10.8～10.9。,。1.2　CODCTL-12COD;BOD5OxiTopBOD5;,(30～60℃);GB1893-1989;GB1893-1989;(MLSS)(SS)GB11901-1989[8];Zeta(Zetasizer2000,MalvernInstrumentsLtd.,UK);(ZEISSAxioskop2motplus)。1.3　62LSBR。。pH4、5、6、7、89。,(NH4)2SO4、KH2PO4BOD∶N∶P100∶5∶1。SBR,10h,2h,1/2,。1、2。,(DO)0.5～1.5mg/L。BOD(Ns)1.0kgBOD/kgMLSSd。pH。1.4　SBRpH(SBR)。1,,22.8L。、。,pH,COD,SBR。(1h)、(9h,)、(2.5h)(0.5h),1/2。,COD9500～24000mg/L,3300～14000mg/L,Ns1.0kgBOD/kgMLSSd(),pHpH5。100d。1　SBR-Fig.1　SchematicdiagramoftheSBR-yeastsystem2　2.1　pHpH1。,(pH7～9),pH=8,。pH5～6,,25mm。pH,。pH7,,NaOHpH,,。,,,,。,pH9575～6。1　pHTable1　EffectofpHonfoam-forminginwastewatertreatmentpH(mm)433.3±1.5525.7±0.6625.3±0.67115.7±2.58206.3±1.59152.7±3.12.2　pHCODMLSS1,pHCOD2。pH,pH4～7。,COD,pH7～9。COD10000～12000mg/L,pH7～9CODpH4～63,1000～1100mg/L。pH7,,。,pH4～6,COD400mg/L。,,pH5～6。2　pHCODMLSSFig.2　EffectsofpHonCODremovalandMLSSinbulksolution,。,2,()。,、,,。。3,,10g/L,pH=7,,7g/L。pH8～92g/L,28%15%。pH7～9COD。3　pHMLSSFig.3　ComparisonofeffectivebiomassandtotalbiomassunderdifferentpHconditions2.3　pH2,,4。pH7～9,,,3pH7pH8,,pH,pH9,,。,,,。,pH5,。。,,pH5。2.4　pHpH,,5。,pH。100minpH,。pH8～9,pH1～1.5969:pH4　2pH(×1000)Fig.4　ComparisonofmicroorganismcompositionsindifferentpHsafter2weeks′operation(×1000)。,pH。pH6～71pH,。pH4～5pH,pH5pH3,300min,pHpH4。pH。,pH6～7,pH,pH[9],,,。pH5,pH,5　pHpHFig.5　pHchangesofbulksolutionsineveryreactorwithdifferentinitialpHconditions,pH3,。,,。2.5　pHZetapH,Zeta。2,1,,Zeta,6。,ZetapH,pH4～5pH0。pH,,,。,。,,pH,,,。,pH=5。2.6　pH,SBR。7,100d,COD9500～24000mg/L,3300～14000mg/L,COD88.9%～9776　pH1ZetaFig.6　ZetapotentialsofmixedliquidunderdifferentpHconditionsatendofoneoperationcycle97.5%93.4%～99.9%,。8SBRpH5(SVI)Ns。COD,Ns0.2～2.3kgBOD/kgMLSSd,1.0kgBOD/kgMLSSd。SVI。Ns1.5kgBOD/kgMLSSd,SVI,,SVI。,SVI150mL/g,80mL/g,,pH5、。7　SBRpH=5CODFig.7　RemovalratesofCODandoilintheSBRoperatedunderpH=5condition3　　SBR、、、、pH、Zata8　SBRpH=5SVIBODFig.8　TheSVIandBODloadintheSBRoperatedunderpH=5condition,pH5。100dSBR,pH=5,。[1]GurloisP.,AlricG.,PoroclionJ.P.,eta.lTheelimina-tionoffatsbyanaerobicbiologicaltreatmen.tTech.Sc.iMeth.,1993,5:247～251[2]AngelidakiI.,AhringB.K.Effectsoffreelong-chainfattyacidsonthethermophilicanaerobicdigestion.App.lMicor-bio.lBiotechno.l,1992,37:808～812[3]ChigusaK.,HasegawaT.,YamamotoN.,eta.lTreatmentofwastewaterfromoilmanufacturingplantbyyeasts.WaterScienceandTechnology,1996,34(11):51～58[4]OswalN.,SarmaP.M.,ZinjardeS.S.,eta.lPalmoilmilleffluenttreatmentbyatropicalmarineyeas.tBiore-sourceTechnology,2002,85(1):35～37[5]ShaokuiZheng,MinYang,ZhifengYang.Biomassproduc-tionofyeastisolatefromsaladoilmanufacturingwastewater.BioresourceTechnology,2005,96(10):1183～1187[6],,..,2001,21(4):347～350[7],,,..,2004,24(1):16～20[8].(3).:,1997[9],,..,2002,24(3):38～4098