不同氮源对4种海洋微藻生长的影响

2010,19(10):[e-mail](4077607840876074)1982E-mail:[e-mail]*2010-10-09////5106324HeterosigmaakashiwoKareniasp.PhaeocystisglobosaChaetocerossp.40.450.520.70d-10.65d-14X173Q948A1674-5906201010-2452-06[1][1-4][5](Dissolvedinorganicnitrogen,DIN)(NO3--NNO2--NNH4+-N)(Dissolvedorganicnitrogen,DON)()[6-7][6][8][9]()[10-11]()[12-14][15][16]HeterosigmaakashiwoKareniasp.PhaeocystisglobosaChaetocerossp.NO3--NNH4+-N(CC275TL2H)(24±1)120µmol·m-2·s-112L12D(30.5)[17]f/2[18]100µmol·L-1(N)4242453(205µmol·L-1)100µmol·L-13f/250mL(PYREX,USA)35mL330251220(a)10minTD-700(TurnerDesigns)a(RelativeFluorescenceUnit,RFU)[19]RFU[20]1010LnLnNNTTμ−=−μ(d-1)N1N0T1T0aOrigin7.0SPSS13.04(1,2)1daa0.450.440.330.37d-1(p0.05)(p0.01)4231d3da0.52d-1(p0.05)(p0.01)(24)1da6d4d00.20.40.60.8H.akashiwoKarensp.P.globsaChaetocerossp.比生长速率/d-1ControlNitrateAmmoniaUreaAminoacidmixture2Fig.2SpecificgrowthratesofH.akashiwo,Kareniasp.,P.globosa,andChaetocerossp.withnitrate,ammonia,ureaandaminoacidmixture0100200300400500600700012345678t/d相对叶绿素a荧光单位ControlNitrateAmmoniumUreaAminoacidMixture1Fig.1GrowthcurvesofH.akashiwowithnitrate,ammonia,ureaandaminoacidmixture245419102010100.650.630.650.33d-1(p0.01)(p0.01)2541d3d3d0.70.670.670.41(p0.01)(p0.05)(p0.05),(p0.05)(p0.01)NO3--NNH4+-N[21]NO3--NNH4+-N4NH4+-NGS/GAGOTNO3--NNO2--NNH4+-N[22](NO3--N)NO3--N[23-24]()NO3--NNH4+-N[25-26]NO3--NNO2--NNH4--N[27][5]NO3--NNH4+-NNO3--NNH4+-NBalode[28]RigaNH4+-NNO3--NNH4+-N7.5μmol·L-1NO3--N050100150200250300350400450012345678t/d相对叶绿素a荧光单位ControlNitrateAmmoniumUreaAminoacidMixture3Fig.3GrowthcurvesofKareniasp.withnitrate,ammonia,ureaandaminoacidmixture0100200300400500600700123456789t/d相对叶绿素a荧光单位ControlNitrateAmmoniumUreaAminoacidMixture4Fig.4GrowthcurvesofP.globosawithnitrate,ammonia,ureaandaminoacidmixture0100200300400500600700800012345678t/d相对叶绿素a荧光单位ControlNitrateAmmoniumUreaAminoacidMixture5Fig.5GrowthcurvesofChaetocerossp.withnitrate,ammonia,ureaandaminoacidmixture42455[29]DONDON[30]NDON[16]Berman[6]19949DON380µg·L-1DIN42µg·L-1Mulholland[31]2002411QuantuckBay,LongIslandDON13.6~36.1µmol·L-1DIN1118.48µmol·L-1(DON80%)4100.34~2.05µmol·L-1DON1%~10%DONMulholland[32]LafayetteRiverVADON17.4~52.3µmol·L-1DIN0.4~11.2µmol·L-1DONµg·L-1[33][34]µg·L-1Chen[35](PHAA)(THAA)(DFAA)0.41~12.61.1~4.00.15~1.10μmol·L-1Yang[36]2006441DFAADFAA0.13~1.62μmol·L-1(0.57±0.05)μmol·L-DFAA0.22~2.6μmol·L-(0.94±0.08)μmol·L-[37]NH4+H2CO3Aureococcusano-phagefferens[25,38-40]NH4+-N[31-32,41]Pseudo-nitzschiaaustralisNH4+-NNO3--NNO3--N-2NH4+-N3[42]DON[43][25]NO3--NNH4+-N[1]ANDERSONDM,GLIBERTPM,BURKHOLDERJM.Harmfulalgalbloomsandeutrophication:nutrientsources,composition,andconsequences[J].Estuaries,2002,25(4):704-726.[2]ANDERSONDM,BURKHOLDERJM,COCHLANWP,etal.Harmfulalgalbloomsandeutrophication:ExamininglinkagesfromselectedcoastalregionsoftheUnitedStates[J].HarmfulAlgae,2008,8:39-53.[3],,,.3[J].,2008a,32(4):482-487.HUZhangxi,XUNing,DUANShunshan,etal.EffectsofureaonthegrowthofPhaeocystisglobosa,Scrippsiellatrochoidea,Skeletonemacostatum[J].ActaScientiaeCircumstantiae,2010,30(6):1265-1270.[4]XUN,DUANSS,LIAF,etal.Effectsoftemperature,salinityandirradianceonthegrowthoftheharmfuldinoflagellateProrocentrumdonghaienseLu[J].HarmfulAlgae,2010,9(1):13-17.[5]YINK,HARRISONPJ.NitrogenoverenrichmentinsubtropicalPearlRiverestuarinecoastalwaters:possiblecausesandconse-quences[J].ContinentalShelfResearch,2008,28:1435-1442.[6]BERMANT.DissolvedorganicnitrogenutilizationbyanApha-nizomenonbloominLakeKinneret[J].JournalofPlanktonResearch,1997,19(5):577-586.24561910201010[7]BERMANT,CHAVAS.Algalgrowthonorganiccompoundsasnitrogensources[J].JournalofPlanktonResearch,1999,21(8):1423-1437.[8]DOBLINM,LEGRANDC,CARLSSONP,etal.UptakeofhumicsubstancebythetoxicdinoflagellateAlexandriumcaten-ella[C]//HALLEGRAEFFGM,BLACKBURNSI,BOLCHCJ,etal,eds.HarmfulAlgalBlooms.IntergovernmentalOceanographiccom-missionofUNESCO,2000:336-339.[9]LEGRANDC,JOHNSENG,GRANÉLIE,etal.EffectofpolyamineongrowthandtoxicityofChrysochromulinaleadbeateri(Haptophyte)[C]//HALLEGRAEFFGM,BLACKBURNSI,BOLCHCJ,etal,eds.HarmfulalgalBlooms.IntergovernmentalOceanographiccom-missionofUNESCO,2000:332-335.[10]OGATAT,KOIKEK,NOMURAS,etal.UtilizationoforganicsubstanceforgrowthandtoxinproductionbyAlexandriumtama-rense[C].//YASUMOTOT,OSHIMAY,FUKUYOY,eds.HarmfulandToxicAlgalBlooms.IntergovernmentalOceanographiccommis-sionofUNESCO,1996:343-346.[11]ARZULG,SEGUELM,CLÉMENTA.Effectofmarineanimalexcretionsondifferentialgrowthofphytoplanktonspecies[J].ICESJournalofMarineScience,2001,58:386-390.[12]BRONKDA,GLIBERTPM,WARDBB.Nitrogenuptake,dis-solvedorganicnitrogenreleaseandnewproduction[J].Science,1994,265:1843-1846.[13]BRONKDA,WARDBB.GrossandnetnitrogenuptakeandDONreleaseintheeuphoticzoneofMontereyBay,California[J].Limnol-ogyandOceanography,1999,44:573-585.[14]CONDONRH,STEINBERGDK,BRONKDA.Productionofdissolvedorganicmatterandinorganicnutrientsbygelatinouszoo-planktonintheYorkRiverestuary,ChesapeakeBay[J].JournalofPlanktonResearc