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1,2,1,3,4(1.,214122;2.,214122;3.,222005;4.,214122):,.,A,.A10molPL,1918%13310%4512%.,4.48d,56%,,,,.A,,10molPLA,(VSS)F4201120molPg1152molPg.:;;:X703.1:A:025023301(2009)0621701204:2008207209;:2008208218:(973)(2007CB714036):(1968),,,,E2mail:xfli@jiangnan.edu.cnEffectofMetalChelatingAgentonAnaerobicDigestionUnderDifferentSubstratesLIXiu2fen1,2,HUQing2hao1,3,CHENJian4(1.SchoolofEnvironmentalandCivilEngineering,JiangnanUniversity,Wuxi214122,China;2.KeyLaboratoryofIndustrialBiotechnology,MinistryofEducation,JiangnanUniversity,Wuxi214122,China;3.SchoolofChemicalEngineering,HuaihaiInstituteofTechnology,Lianyungang222005,China;4.NationalKeyLaboratoryofFoodScienceandTechnology,JiangnanUniversity,Wuxi214122,China)Abstract:AidedbyGCanalysis,theeffectofmetalchelatingagentonanaerobicdigestionunderdifferentsubstrateswasinvestigated.TheresultsshowedthattheadditionofchelatingagentAcouldgreatlypromotethemethaneproductionunderdifferentsubstratesandspeedtheconversionoforganicacidtomethane,especiallyforpropionicacid.WhenthedosageofchelatingagentAwas10molPL,themethaneproductionwas,respectively,1918%,13310%and4512%forbutyricacid,propionicacidandaceticacid.Also,thearrearagetimeofmethaneproductionwasshortenedandtheproductionrateincreasedby42fold.During428doperation,thedegradationrateofbutyricacidreached56%.TheaccumulationofaceticacidwasnotdetectedbyGCanalysis,whichdemonstratedaceticacidconvertedfrombutyricacidwasquicklyutilizedbymethaneproducingbacteria.TheincreaseinmethaneproductionduetotheadditionofchelatingagentAcouldbesupportedbyenzymology.Takingaceticacidasanexample,thecontentofcoenzymeF420insludgeincreasedto1152molPgfrom1120molPgduetotheadditionof10molPLchelatingagentA.Keywords:anaerobicdigestion;organicacid;metalchelatingagent,(volatilefattyacid,VFA),,[14].[5],FeCoNi,,,,[68],,,.,3,A[9],.11.11.1.1.pH712,VSSPTSS0172.2L,352,5d,2.1.1.2,30620096ENVIRONMENTALSCIENCEVol.30,No.6Jun.,20091.2[10].011mLPL.1.2125mL90mL,5min,.(VSS)5gPL,pH710715,352.A,A,10molPL.1.31PmgL-1Table1ComponentsofsyntheticwastewaterPmgL-1PPNH4ClKH2PO4MgSO47H2OCaCl22H2ONa2S7000P3600P310002005510010782Table2ComponentsofthetraceelementsolutionPmgL-1FeCl24H2O200092ZnCl256232MnCl24H2O502000CuCl22H2O24495NiCl26H2O50050CoCl26H2O13927(NH4)6Mo7O24H2O38164Na2SeO25H2O14491.3.12molPLNaOH,,.,12h.1.3.2VFA(SP2000)(FID)(015mm30mm,PEG220M).210.,20mLPmin.:602min,5Pmin160,1605min.:4800rPmin10min,15000rPmin10min,012m,3molPLH3PO4,42.F420[11]..22.1A3,,,A,,1918%13310%4512%,.,3COD,,.3Table3AccumulativemethaneproductionbyuseofdifferentsubstratesPmLAPmLP%18221819181842133101351964512:A,.3,,,A,;A.,,,35%.(),,,[12,13].2.2A1A.,,A,,313,2071301AFig.1EffectofchelatingagentonthemethaneproductionratebyuseofdifferentsubstratesA.,.,,,1d,29mLPd,.,4d,,8d,45mLPd.,6d,8d,,3mLPd,,20mL,.3.,1d,.,,,.:CH3CH2COOH+2H2OCH3COOH+CO2+3H2(1)G0=7611kJPmolCH3CH2CH2COOH+2H2O2CH3COOH+2H2(2)G0=48.1kJPmol,(1105Pa),(1)(2),,,.,,,.,,.A,,4.2.3A,2.A,.,4d,.4d,3000mgPL2500mgPL,17%,4d,1100mgPL,56%.,,,.2Fig.2Substrateconcentrationduringanaerobicdigestionbutyricacid2.4AF420F420(E-340-350mV)[14].,F420,30716:NADPHCO2,CH4.,F420.[15]UASB,F420;[16]F420.,,AF420,A,F4201120molPg,10molPLA,F4201152molPg.A.3(1),A.1918%13310%4512%.(2),.(3)A,,10molPLA,F4201120molPg1152molPg.:[1]BabelS,FukushiK,SitanrassameeB.Effectofacidspeciationonsolidwasteliquefactioninananaerobicaciddigester[J].WatRes,2004,38(9):241622422.[2]VeekenAHM,HamelersBVM.Effectoftemperatureonhydrolysisratesofselectedbiowastecomponents[J].BioresourceTechnol,1999,69(3):2492254.[3]HoriuchiJ,ShimizuT,TadaK,etal.SelectiveproductionoforganicacidsinanaerobicacidreactorbypHcontrol[J].BioresourceTechnol,2002,82(3):2092213.[4],RalfC,.[J].,2001,1(2):1802184.[5]HongKJ,TokunagaSYI.ExtractionofheavymetalsfromMSWincineratorflyashusingsaponins[J].Chemosphere,2000,41(3):3452352.[6],.[J].,2001,23(3):1162118.[7]PatidarSK,TareV.Effectofnutrientsonbiomassactivityindegradationofsulfateladenorganics[J].ProcessBiochem,2006,41:4892495.[8]GracielaG,RobbertK,LettingaG.EffectofnickelandcobaltonkineticsofMethanolconversionbymethanogenicsludgeasassessedbyon2lineCH4monitoring[J].ApplEnvironMicrobiol,1999,65(4):178921793.[9]HuQH,LiXF,LiuH,etal.EnhancementofmethanefermentationinthepresenceofNi2+chelators[J].BiochemEngJ,2008,38(1):982104.[10]MarcelHZ,GeertsR,LettingaG.Methanoldegradationingranularsludgereactorsatsub2optimalmetalconcentrations:roleofiron,nickelandcobalt[J].EnzymeMicrobiolTechnol,2003,33:1902198.[11],,,.[J].,2007,26(5):71274.[12],.[J].,2002,32(1):83289.[13],,.[J].,2005,21(3):25227.[14]WarkentinE,HagemeierC,ShimaS,etal.TheStructerofF4202dependentmethylemetetranydromethanopterindehydrogenase:acrystallographicsuperstructureoftheSelenomethionire2labelledProteinCrystalStructure[J].BiologicalCrystallography,2005,61:1982202.[15],,.[J].,2005,25(3):3792384.[16],,,.[J].,1998,16(3):8212.407130