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34520135ENVIRONMENTALSCIENCEVol．34No．5May2013*200240．、Na2SNa2S2O33．NO－3-N13mg·L－1Na2S2O3Na2SNa2S2O3．20℃HRTHRT≥2hNO－3-NTN81%79%HRT＜2hNO－3-NTN47%51%NO－2-N0.53mg·L－1Na2SHRT4hNO－3-NTN47%41%NO－2-N0.29mg·L－1Na2S2O3NO－3-NTN99%90%NO－2-N0.080mg·L－1HRT0.5h．3Na2SNa2S2O3．X703A0250-3301201305-1835-102012-08-012012-09-272008ZX07106-2-2NCET-11-03201988～E-mailhelloyy2006@126.com*E-mailweilizhou@sjtu．edu．cnStudyonSulfur-basedAutotrophicDenitrificationwithDifferentElectronDonorsYUANYingZHOUWei-liWANGHuiHESheng-bingSchoolofEnvironmentalScienceandEngineeringShanghaiJiaoTongUniversityShanghai200240ChinaAbstractSulphur-basedautotrophicdenitrificationwasappliedtotreatthelowconcentrationnitrate-contaminatedwater．Differentelectrondonorsnamelyelementalsulfursulfideandthiosulfatewereusedinthreecontinuousreactorstocomparethedenitrificationperformance．Whentreatingthelowconcentrationnitrate-contaminatedwater13mg·L－1thethiosulfatesystemshowedthebestperformanceandthesulfidesystemwastheworst．Thethiosulfatesystemwaslesssensitivetolowtemperaturethantheothertwo．Attemperatureshigherthan20℃thesulfursystemwasgreatlyinfluencedbymasstransferefficiencyandHRT．Itremoved81%ofnitrateand79%ofTNwhenHRTwasnolessthan2hbutcouldonlyachievealownitrateandTNremovalrateof47%and51%whenHRTwasshorterthan2h．Noobviousnitriteaccumulationwasobservedandtheaverageeffluentnitritewas0.53mg·L－1．Thesulfidesystemcouldonlyremove47%ofnitrateand41%ofTNwith0.29mg·L－1nitriteintheeffluentattheHRTof4h．Meanwhilethethiosulfatesystemhadahighremovalrateof99%fornitrateand90%forTNwithalowcontentofeffluentnitriteof0.080mg·L－1andtheHRTcouldbeshortenedto0.5h．ThemolecularbiologicalanalysisshowedthatdifferentbacteriapredominatedinthethreereactorsandthatThiobacillusdenitrificansexistedabundantlyinthesulfursystemwhilethefunctionalbacteriainthesulfideandthiosulfatesystemscouldnotbeidentifiedyet．Anewspeciesofsulfur-basedautotrophicdenitrificationbacteriummayhavebeenfound．Keywordssulfur-basedautotrophicdenitrificationelectrondonornitratephylogenetictreeThiobacillusdenitrificans、、、1～7．8．S2－、S0S2O2－3．9/3h80%．Sierra-Alvarez10/NO－3-N1.3mmol·L－118.1mmol·L·d－1NO－3-N95.9%NO－2-NS2－．DeLomas11NutrioxTMH2S98.7%94.7%341T．denitrificans．12、、99%．13Na2S2O30.96kg·m3·d－190%NO－3-NNO－2-N．．314151617、18pH．、．3、、．11.1388mm500mm3.2L．、、、、51．3、Na2S、Na2S2O3．2.5L5～15mm1∶1.5Na2S200g2.0L1～2mmNa2S2O32.0L2.0L4～5mm1.4L、＜4mm0.6L．、．1Fig．1Experimentalset-up1.2．Na2SNa2S2O3Na2S·9H2ONa2S2O3·5H2ONaHCO3．S∶N=1∶10.84∶1MgCl2·6H2OKH2PO4．1．11Table1Compositionofsyntheticwastewater/mg·L－1NaNO360.7Na2S·9H2ONa2S171.6Na2S2O3·5H2ONa2S2O3177.1NaHCO3Na2S、Na2S2O366.9KH2PO43.3MgCl2·6H2O6.01NO－3-N10mg·L－11.3310～30℃pH6.0～7.0HRT3．1.4NO－3-NNO－2-NN-1--TN63815SO2－419pH．1.5－80℃DNA2021．DNADNA．PCR27F/927R22nirSnirK23．PCR8%、20%～60%DGGE24DNA、PCR、、NCBI．23NO－3-N10mg·L－1NO－3-N3mg·L－1NO－3-N13mg·L－1．10～20℃20℃．2.1NO－3-N2～4NO－3-N．2NO－3-NFig．2RemovalofNO－3-Nintheelementalsulfursystem3Na2SNO－3-NFig．3RemovalofNO－3-NinthesulfidesystemNO－3-N2.10～20℃HRT2～4hNO－3-N5.2mg·L－1NO－3-N50%．20℃HRT4h2hNO－3-NNO－3-N90%．NO－3-N0.60mg·L－194%．HRT3h1.5hNO－3-N40%．NO－3-N4.9mg·L－151%．38d．HRT1.5h3hNO－3-N90%HRT7381344Na2S2O3NO－3-NFig．4RemovalofNO－3-NinthethiosulfatesystemNO－3-N2.2mg·L－177%．20℃、HRT2hNO－3-N81%HRT＜2hNO－3-N47%．HRT．5NO－2-NFig．5VariationofNO－2-NintheelementalsulfursystemNa2SHRT4h．310～20℃NO－3-N6.9mg·L－1NO－3-N46%．20℃NO－3-N6.8mg·L－1NO－3-N47%．Na2SNO－3-N．．Na2S2O3NO－3-N4.10℃～20℃HRT6h4hNO－3-N1.0mg·L－1NO－3-N92%．20℃HRT1.5h0.5hNO－3-N100%．Na2S2O3HRT．3Na2S2O3．2.2NO－2-NSO2－4NO－2-NNO－2-N．5～7NO－2-N．10～20℃NO－2-N3a1.0～3.0mg·L－120℃NO－2-N0.2～0.8mg·L－1．Na2S610～20℃NO－2-N1.0mg·L－1．20℃NO－2-N0.29mg·L－1．Na2S2O3710～20℃NO－2-N0.39mg·L－1．20℃NO－2-N0.080mg·L－10.60mg·L－1．HRTNO－2-N．3Na2S2O3NO－2-N．8～1020℃SO2－4．、Na2S、Na2S2O3838156Na2SNO－2-NFig．6VariationofNO－2-Ninthesulfidesystem7Na2S2O3NO－2-NFig．7VariationofNO－2-Ninthethiosulfatesystem8SO2－4Fig．8ConcentrationofeffluentSO2－4intheelementalsulfursystemSO2－4224、156174mg·L－1．．．3SO2－4250mg·L－125．2.3TNNO－3-NNO－39Na2SSO2－4Fig．9ConcentrationofeffluentSO2－4inthesulfidesystemNO－2N2TN．11～13TN．TNHRT11．10～20℃HRT2～4hTN9.7mg·L－1TN40%．20℃TN88%TN1.5mg·L－1HRT3h1.5hTN55%TN75%．20℃93813410Na2S2O3SO2－4Fig．10ConcentrationofeffluentSO2－4inthethiosulfatesystem、HRT≥2hTN79%HRT＜2h51%．Na2STN12HRT4h．10～20℃TNTN36%20℃TN41%．Na2S2O3TN13HRTHRTTN．10～20℃TN2.6mg·L－1TN80%20℃TN1.3mg·L－1TN90%．310～20℃20℃2．10～20℃Na2SNa2S2O3NO－3-NTN92%80%NO－2-N0.39mg·L－1．Na2S2O3．HRT．Na2S2O3NO－3-NTN99%90%NO－2-N0.080mg·L－1HRT0.5h．11TNFig．11RemovalofTNintheelementalsulfursystem12Na2STNFig．12RemovalofTNinthesulfidesystem32～132Na2S2O3HRTHRT2h0481513Na2S2O3TNFig．13RemovalofTNinthethiosulfatesystemNa2SHRT4h10～20℃20℃．．26/20℃、2～3mg·L－1HRT15minNO－3-N90%0.1459kg·m3·d－17.8mg·L·h－10.10·t－1．Na2S2O3HRT30minNO－3-N99%0.4795kg·m3·d－125.9mg·L·h－10.18·t－1．HRT．23Table2Comparisonofthetreatmentefficiencyoftheautotrophicdenitrificationreactorswithdifferentelectrondonors10～20℃20℃SNa2SNa2S2O3SNa2SNa2S2O3NO－3-N/%504692734799TN/%403680724190NO－3-N/mg·L－15.26.91.02.76.80.010TN/mg·L－19.78.22.63.57.61.3NO－2-N/mg·L－12.21.00.390.530.290.080HRT/h—44240.52.43DNAPCR-DG