不同电子受体对反硝化除磷菌缺氧吸磷的影响刘建广

,,,(,　250101):,,(DPB).,,DPB,,1mgNO-3-N1mgPO3-4-P;,DPB,DPB(NO-2-N5～20mgL),NO-2-N;DPB,NO-2-N≥35mgL,DPB.:;;;:X703.1　:A　:0250-3301(2007)07-1472-05:2006-07-27;:2006-10-31::(1964～),,,,,E-mail:[e-mail]orsonAnoxicPhosphorusUptakeofDPBLIUJian-guang,FUKun-ming,YANGYi-fei,ZHANGWei-jian(SchoolofPublicWorks&EnvironmentalEngineering,ShandongJianzhuUniversity,Ji′nan250101,China)Abstract:Thetestofanoxicphosphateuptakeofdenitrifyingphosphorousremovalbacteria(DPB)whichwascultivatedduringtheanaerobicanoxicaerobicprocesseswasconductedatthevarioussituationsofelectronacceptors.Thevariousconcentrationsofnitrateornitritewereaddedduringtheanoxicstagerespectively.TheconclusionsstatedthattheanoxicphosphateuptakeofDPBwasrarelyinfluencedbytheconcentrationofnitratewiththeadequatenitrateaselectronacceptors.Ittakes1mgPO3-4-PwhentheconsumptionofNO-3-Nis1mg.Thenitritecouldberegardedastheelectronacceptortoparticipateintotheactivitiesofdenitrifyingphosphorousremoval.Comparedwiththenitrate,thephosphorousuptakerateofDPBwithnitritewasratherhigheratalowconcentration(NO-2-Nwiththeconcentrationrangeof5～20mgL).Furthermore,therateofanoxicphosphorousuptakewasincreasedwiththedecreasedconcentrationofNO-2-N.TherestrainingeffectsrelatedtotheanoxicphosphorsuptakeofDPBwasincreasedastheincreaseofnitriteconcentration.Hence,theanoxicphosphorousuptakeofDPBwasentirelyinhibitedwhentheconcentrationofNO-2-Nwashigherthan35mgL.Keywords:nitrate;nitrite;anoxicphosphorusuptake;denitrifyingphosphorusremoval　　,、,,.,,,,;,,BOD,,、.2090,Kuba[1],[2～5],,,O2,NO-3;Kerrn-Jespersen[6]2:1O2,1O2.“”,,,,,.50%COD、30%50%[7].,.,,,.,、.28720077　　　　　　ENVIRONMENTALSCIENCEVol.28,No.7Jul.,2007DOI:10.13227/j.hjkx.2007.07.0111　1.1　SBR,,15L.,(1).1.;2.;3.;4.SBR;5.;6.1　SBRFig.1　SchematicofSBR　,(NH4)2HPO4.,,NaOHHClpH.1.1　Table1　QualityofsyntheticwaterCODmg·L-1NH+4-Nmg·L-1pHPO3-4-Pmg·L-1200～4009.0～13.57.0～7.510～151.2　COD、PO3-4-P、NO-3-N、NO-2-N、MLSS,[8],pHPHS-3BpH,DOHI9143.2　2.1　.2,1,2.[9],,10%56.8%,94.8%.1-,2h、3h,2～3mgL.2,15～22d,MLSS=4000mgL.60d,.PO3-4-P1mgL,93%,2.2,,PO3-4-P9.1mgL25.9mgL,.,PO3-4-P0.7mgL.,,.2　-Fig.2　Variationofphosphateconcentrationunderanaerobicaerobicprocess　3　--Fig.3　Variationofphosphateandnitrateconcentrationunderanaerobic-anoxic-aerobicprocess2--,2h、3h、1h.,NO-3-N50mgL.2,15～22d,MLSS=4000mgL.,,40d3.3,,,PO3-4-P14737:25mgL,,PO3-4-P1mgL,.2.2　2:1(Ⅰ),1(Ⅱ),2SBR.,SBR2h,(,SBRPO3-4-PCOD.COD,,COD,DPB;,,,,),,2,HClpH7.2.Ⅰ:61L,1～6,,,100rmin,25℃.,61、2、3、5、7、10mLKNO3(NO-3-N=10gL),NO-3-N10、20、30、50、70、100mgL.1、2、3h,NO-3-NPO3-4-P.Ⅱ:6250mL,1～6,,0.2、0.4、0.6、0.8、1.4、2.0mL(NO-2-N=6.25gL),NO-2-N5、10、15、20、35、50mgL.10min,PO3-4-PNO-2-N.2.2.1　Ⅰ45.4PO3-4-P,5NO-3-N.4、5,NO-3-N30mgL,1h,,1NO-3,,PO3-4-P,31.65mgL15.64mgL,2h.NO-3-N≥30mgL,1h,4　Fig.4　Variationofphosphateconcentrationundervariousnitrateconditions　5　Fig.5　Variationofconcentrationsofnitrate　,,.27.43mg(L·h),,2h.1h,23PO3-4-P,23NO-3-N,,.2h,4～6PO3-4-P,NO-3-N,.2:①,;②.,56COD10mgL,COD,.5,,NO-3-N,,27.70mg(L·h).,1mgPO3-4-P1mgNO-3-N..1474　　　　　　28Kerrn-Jespersen[6],1mgNO-3-N2.0mgPO3-4-P,[10]NO-3-N0.80mgmg,[11]PO3-4-PNO-3-N1.51.,,.2.2.2　ⅡNO-2-N,10min6.6PO3-4-P,NO-2-N.6　NO-2-NFig.6　PhosphateuptakeandnitriteremovalunderdifferentNO-2-Nconcentration　6,NO-2-N.NO-2-N5mgL,10minPO3-4-P,13.1mgL.NO-2-N,,NO-2-N;NO-2-N,NO-2-N20mgL,4.7mgL,NO-2-N5mgL36%,.NO-2-N≥35mgL,,,.,NO-2-N20～35mgL,.,,,.NO-2-N5mgL,10min78.6mg(L·h),.,,,NO-2-N20mgL,28.2mg(L·h),.Lee[12],,.,,,,.,,,NO-2-NNO-2-N,NO-2-N15mgL,,7.52mgL;50mgL,5.91mgL.,2,1,,1,,,.,,,,,NO-2-N,,,.,.Kuba[7],NO-2-N(5～10mgL);Meinhold[13]NO-2-N4mgL,,NO-2-N8mgL,,,,;Lee[8]SBR,,NO-2-N10mgL,;Hu[14],,,NO-2-N115mgL.,,pH、、MLSS、.,[15],HNO2,.Sijbesma[16]Yarbrough14757:[17];,pH,NO-2H+HNO2,,pH,pH,ATP.ATPADP,ATP,ATPADP,,.,,,,,.3　　　(1),NO-3-N.,1mgNO-3-N1mgPO3-4-P.(2).NO-2-N(5～20mgL),,NO-2-N.(3),.(4)NO-2-N,NO-2-N≥35mgL,.:[1]KubaT,SmoldersG,vanLoosdrechtMCM,etal.Biologicalphosphorusremovalfromwastewaterbyanaerobic-anoxicsequencingbatchreactor[J].WatSciTech,1993,27(5,6):241～252.[2]KongYH,NielsenJL,NielsenPH.MicroautoradiographicStudyofRhodocyclus-RelatedPolyphosphate-AccumulatingBacteriainFull-ScaleEnhancedBiologicalPhosphorusRemovalPlants[J].ApplEnvironMicrobiol,2004,70(9):5383～5390.[3]TsunedaS,OhnoT,SoejimaK,etal.Simultaneousnitrogenandphosphorusremovalusingdenitrifyingphosphate-accumulating　　　　　　organismsinasequencingbatchreactor[J].BiochemEnginJ,2006,27(3):191～196.[4]ZengRJ,LemaireR,YuanZH,etal.Simulataneousnitrificationdenitrificationandphosphorusremovalinalab-scalesequencingbatchreactor[J].BiotechnolBioeng,2003,84(2):170～178.[5]TsunedaS,MiyauchiR,OhnoT,etal.Characterizationofdenitrifyingpolyphosphate-accumulatingorganismsinactivatedsludgebasedonnitritereductasegene[J].JBiosciBioeng,2005,99(4):403～407.[6]Kerrn-JespersenJP,HenzeM.Biologicalphosphorusuptakeunderanoxicandaerobicconditions[J].WatRes,1993,27(4):617～624.[7]KubaT,vanLoosdrechtMCM,HeijnenJJ.PhosphorusandnitrogenremovalwithminimalCODrequirementbyintegrationofdenitrifyingdephosphatationandnitrificationinatwo-sludgesystem[J].WatSciTech,1996,30(7):1702～1710.[8]《》.[M]