不同负荷对厌氧氨氧化污泥培养的影响侯晓帮

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:2017-05-31:(1985-),,,,,.1,2(1.,210008;2.,210098):(ANAMMOX),,ANAMMOX。40mg/(L·d),ANAMMOX200d;18mg/(L·d),55d,ANAMMOX。ANAMMOX。,HRT2.0d、24h,55mg/(L·d),Vmax=0.00517h-1(ρ(NH4+-N)/ρ(SS)),KS=58.3(ρ(NH4+-N))。:;;;:X5:A:1674-4829(2017)04-0028-05TheInfluenceofDifferentLoadsonANAMMOXCulturingHOUXiao-bang1,CAOJia-shun2,(1NanjingMunicipalDesignandResearchInstituteCo.Ltd.,Nanjing210008,China;2HohaiUniversity,Nanjing210098,China)Abstract:MethodofloadtransformationhadbeenusedinthecultureofANAMMOXbacterium.Theactivityhadbeenweakfor200dundertheconditionof40mg/(L·d)loading,whilecultureonly55dintheconditionsof18mg/(L·d)loading,ANAMMOXbacteriagrewsignificantly.Theresultsshowedthat:low-loadisconducivetothecultivationofANAMMOXbacteria,loadingof55mg/(L·d)issuitablewhenHRTis2.0d,cyclefortheprocessis24h,Vmax=0.00517h-1(ρ(NH4+-N)/ρ(SS)),KS=58.3(ρ(NH4+-N)).Keywords:Anaerobicammoniumoxidation;Enrichment;Hydraulicretentiontime;Load0(AnaerobicAmmoniumOxidation,ANAMMOX)ANAMMOX,,[1-2],。ANAMMOX(11d)[3],,ANAMMOX。,ANAMMOX(Archaea)(Thermotoga)(Aquifex)[2]。ANAMMOX()、[4-6],ANAMMOX,ANAMMOX;,ANAMMOX。ANAMMOX()、,ANAMMOX,ANAMMOX。ANAMMOX。11.11.1.1EGSB(Extendedgranularsludgebed)7.8L,6.5L。,,CO2Ar。。1。30420178EnvironmentalScienceandTechnologyVol.30No.4Aug.2017304312319.271.591.970.687.998.7/%NH4+-NNO2--N1∶5.101∶1.511∶1.28ρ(NH4+-N)∶ρ(NO2--N)NO3--NANAMMOXANAMMOXANAMMOX1.1.21.75LANAM-MOX。1.1.3,1。1L1mL。:FeSO4·7H2O,5g/L;EDTA,5g/L;:CuSO4·5H2O,4g/L;ZnSO4·7H2O,0.43g/L;CoCl2·6H2O,0.24g/L;MnCl2·4H2O,0.99g/L;(NH4)6Mo7O24·4H2O,0.16g/L;NiCl2·6H2O,0.19g/L;H3BO4,0.014g/L;EDTA,15g/L。1EGSB1ρ(NH4+-N)/(mg·L-1)45ρ(NO2--N)/(mg·L-1)60ρ(NaHCO3)/(mg·L-1)420ρ(KH2PO4)/(mg·L-1)27.2ρ(MgSO4·7H2O)/(mg·L-1)300ρ(CaCl2·2H2O)/(mg·L-1)1361.2:、。30min,。15.7L/h(2m/h),32℃,pH8.30。2,110h50min,270h50min,322h50min。1.3265d,NH4+-N48mg/L,NO2--N62mg/L。,3,2。21.4NO2-N,NH4+-N,NO3--N(APHA,1998);pHHACHpH。22.1ANAMMOX2.1.133。1233465789101.;2.;3.;4.;5.;6.;7.;8.;9.;10.230min10min30min/L1.02.52.5HRT/d2.562/℃323232CO2ArAr/(mg·L-1·d-1))401855123716~131131~326326~4102005515127224/d/h292017841、233,1,ANAMMOX(N,)40mg/(L·d)200d,;218mg/(L·d)55d,ANAMMOX。355mg/(L·d),ANAMMOX。3110mg/(L·d),NO2--N76%,NH4+-N28%,ANAMMOX,,218mg/(L·d),18d,NO2--N100%,NH4+-N89%,。2.1.2ANAMMOXC,ANAMMOX[5],2,ANAMMOX,ANAMMOX,600℃ANAMMOX。,。ANAMMOX。2.1.3ANAMMOX[6]:1NH4++1.32NO2-+0.066HCO3-+0.13H+→1.02N2+0.26NO3-+0.066CH2O0.5N0.15+2.03H2ONH4+-NNO2--NANAMMOX,NO3--NANAMMOX。,ANAMMOX,NH4+-N,NO2--NNO3--NANAMMOX。1、234。4,1,ρ(NH4+-N)∶ρ(NO2--N)1∶5.10,NO3--N;2,ρ(NO2--N)∶ρ(NH4+-N)1.51,NO3--N,(a)1NO2--N,NH4+-NNO3--N(b)2NO2--N,NH4+-NNO3--N(c)3NO2--N,NH4+-NNO3--N501001502007065605550454035302520151050ρ/(mg·L-1)HRT/dNO2--NNO2--NNH4+-NNH4+-NNO3--NNO2--NNO2--NNH4+-NNH4+-NNO3--N2322342362382402422442462482502522547065605550454035302520151050ρ/(mg·L-1)HRT/dNO2--NNO2--NNH4+-NNH4+-NNO3--N2522542562582602622642662682707065605550454035302520151050ρ/(mg·L-1)HRT/d3ANAMMOXNH4+-N1001201401601802002202402601086420ρ(NO2--N)∶ρ(NH4+-N)t/d20406080NO2--NNO3--N132303045NH4+-N1215182124t/h0.17136910.008.006.004.002.00NO2--NNH4+-NNO3--NpH70.060.050.030.020.010.00ρ/(NH4+-N),ρ/(NO2--N)ρ/(NO3--N),pH6Xt/(So-Se)1/SeV=Vmax×SeKS+Seρ(NH4+-N)∶ρ(NO2--N)∶ρ(NO3--N)1∶1.51∶0.07;3,ρ(NH4+-N)∶ρ(NO2--N)∶ρ(NO3--N)1∶1.28∶0.16,1∶1.32∶0.26。2.22.2.11,ANAMMOXNO2--N,NH4+-N:①NO2--N;②(dissimilatorynitratereduction),,,[7];③ANAMMOXNO3--NNH4+-N[8]。,ANAMMOX,()ANAMMOX。2,1200d,55dANAMMOX。,[9-10],ANAMMOX、、,,(50%)ANAMMOX[11];ANAMMOX()、[12],,、,ANAMMOX,N18mg/(L·d)ANAMMOXA,ANAMMOX,ANAMMOX。2.2.23,ANAMMOX,5。5,3,NO2--NNH4+-N,NO3--N,pH8.0。,,,24h,HRT2.0d55mg/(L·d),ANAMMOX。ANAMMOX,Monod[13]::V,h-1,V=Sa×SeXt;Vmax,h-1;X,mg/L;S0,mg/L;Set,mg/L;t,h;Ks,V=Vmax2,mg/L。(1),:V=So-SeXt(2):Xt/(So-Se)1/Se,6。6,XtSo-Se=112751Se+193.27,(3)1/Vmax,Ks/Vmax,Vmax=0.00517h-1(ρ(NH4+-N)/ρ(SS)),Ks=58.3mg/L(ρ(NH4+-N))。R2=0.804,XtSo-Se1Se。,。(1)1V=KsVmax×1Se+1Vmax(2)(3)XtSo-Se=KsVmax×1Se+1Vmax0.050.100.150.202500.002000.001500.00100.00500.000Y=11275X+193.27Xt/(So-Se)R2=0.80361/Se3120178(27)[16],,.[J].,2000,19(9):429-431.[17].2-[D].:,2003.[18],,,.[J].,2005(5):26-34.[19],,,.2,4,6-[J].,2007,65(15):1515-1520.[20],,,.[J].,2010,31(1):193-198.[21],,.[J].,2015(9):21-43.[22],.H103[J].,2011,5(8):15-17.[23],,,.[J].,2011,24(S1):22-24.()3(1)40mg/(L·d),18mg/(L·d)ANAMMOX。(2)ANAMMOX,HRT2.0d,24h,N55mg/(L·d)ANAMMOXNH4+-N91.9%,NO2--N98.7%。(3)Monod,ANAMMOX:XtSo-Se=112751Se+193.27。4,,ANAMMOX200d。ANAMMOX,:①40mg/(L·d),CO2;②18mg/(L·d),CO2;③40mg/L·d),Ar;④18mg/(L·d),Ar。【】[1]VANDEGRAAFAA,BRUIJNP.AutotrophicGrowthofanaerobicammonium-oxidizingmicroorganismsinafluidizedbedreactor[J].Microbiology,1996,142(8):2187-2196.[2],,,.[J].,2008,21(5):65-69.[3]STROUSM,HEIJNENJJ,KUENENJG,eta1.These-quencingbatchreactorasapowerfultoolforthestudyofslowlygrowinganaerobicammonium-oxidizingmicroorgan-isms[J].Appliedmicrobiologyandbiotechnology,1998,50(27):589-596.[4]DALSGAARDT,CANFIELDDE,PETERSENJ,etal.N2productionbytheanammoxreactionintheanoxicwatercolumnofGolfoDulce,CostaRica[J].Nature,2003,422:606-608.[5].[D].:,2005.[6]MULDERA,VANDEGRAAFAA,ROBERTSONLA,etal.Anaerobicammoniumoxidationdiscoveredinadenitrify-in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