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2711200711 ActaScientiaeCircumstantiaeVol.27,No.11Nov.,2007:(No.2005CB724203);(No.50478053);(No.04JJ3004,05JJ2004);(No.05FJ3001);(No.K051132-72)SupportedbytheNationalBasicResearchProgramofChina(No.2005CB724203);NationalNaturalScienceFoundationofChina(No.50478053);NaturalScienceFoundationofHunanProvince(No.04JJ3004,05JJ2004);ProjectofScienceandTechnologyofHunanProvince(No.05FJ3001);KeyProjectofScienceandTechnologyofChangsha(No.K051132-72):(1981—),,,E-mail:Wendy810618@163.com;＊(),E-mail:yzh@hnu.cnBiography:WANGrongjuan(1981—),female,E-mail:Wendy810618@163.com;＊Correspondingauthor,E-mail:yzh@hnu.cn,,,.2007.SBBR[J].,27(11):1809-1817WangRJ,YangZH,ZengGM,etal.2007.Impactofdifferentaerationstrategiesonstart-upofshortnitrification-anaerobicammoniaoxidation[J].ActaScientiaeCircumstantiae,27(11):1809-1817SBBR王荣娟1,杨朝晖1,＊,曾光明1,徐峥勇1,浣勇2,刘有胜11.湖南大学环境科学与工程学院,长沙4100822.长沙市固体废弃物处理中心,长沙410202:2006-11-02 :2007-04-13 :2007-09-26:,SBBR.4(a、b、cd16h、12h、12h8h;/4h/2h、3h/3h、2h/2h2h/4h),(30.0±0.5)℃,(DO)(1.2±0.1)mg·L-1.,124d,,,,12h,2h/2hc,96.6%,,0.186g·(L·d)-1;DO,;,,,.4DNA,PCR-DGGEDGGE.、Cs,,.:;SBBR;PCR-DGGE;-;:0253-2468(2007)11-1809-09 :X703.1 :AImpactofdifferentaerationstrategiesonstart-upofshortnitrification-anaerobicammoniaoxidationWANGRongjuan1,YANGZhaohui1,＊,ZENGGuangming1,XUZhengyong1,HUANYong2,LIUYousheng11.CollegeofEnvironmentalScienceEngineering,HunanUniversity,Changsha4100822.CenterforSolidWasteTreatmentofChangsha,Changsha410202Received2November2006; receivedinrevisedform13April2007; accepted26September2007Abstract:Theinfluenceofdifferentaerationstrategiesonthestart-upofshort-cutnitrification-anaerobicammoniaoxidationinfoursequencingbatchbiofilmreactors(SBBRs)wasstudied.Thereactorswereusedfortreatmentoflandfillleachateswithhighconcentrationsofammonianitrogen.Thereactorswerestartedupsimultaneouslyusingfouraerationstrategiesinwhichthefullaerationtimewas16h,12h,12hand8handtheaerobic/anaerobicalternationfrequencywas4h/2h,3h/3h,2h/2hand2h/4h,respectively.Theambienttemperaturewaskeptat(30.0±0.5)℃.Duringtheaerationperiod,thedissolvedoxygen(DO)concentrationwascontrolledat(1.2±0.1)mg·L-1.Theexperimentalresultsshowedthataftera124-day-longperiodofmicroorganismproliferationandacclimation,thereactorswereabletoremoveammonianitrogen,however,theeffectwasdifferentineachreactor.Reactorcwhichusedanaerationstrategywithafullaerationtimeof12handaerobic/anaerobicalternationof2h/2hhadthebesteffectandcouldbestresistshockloading.Theremovalefficiencyandvolumeloadingofammonianitrogenreached96.6%and0.186g(L·d)-1,respectively.Intheaerationperiod,nitriteaccumulatedasaresultofthelimitingDOconcentration.Intheanoxicperiod,anammoxbacteriacooperatedwithdenitrifyingbacteria,sonitriteandammonianitrogenwereeliminatedatthesametimewithoutnitrateaccumulation.AllmicrobialDNAwasextractedfromsamplesDOI:10.13671/j.hjkxxb.2007.11.006 27whichwerecollectedfromnaturallandfillleachateandthebiofilmsofthefourreactors.DGGEmapsoftheextractedDNAwereobtainedthroughPCR-DGGE.Thequantityandbrightnessofeachbandineachlaneofthemapandthecoefficientsofsimilarity(Cs)betweenthelaneswereanalyzed.Theresultsshowedthatthedifferentaerationstrategiesgreatlyinfluencedthebacterialdiversityandcomposition.Keywords:aerationstrategy;SBBR;PCR-DGGE;shortcutnitrification-anaerobicammoniaoxidation;landfillleachate1 (Introduction)(SequencingBatchBiofilmReactor,SBBR)Wilderer,SBBR,SBR,SBR(Irvineetal.,1997),,(),;,、,,、.,,COD,、pH.(shortcutnitrificationdenitrification),Sharon,Delft1997(Hellingaetal.,1998),,.(anaerobicammoniumoxidation,Anammox)DelftKluyver20(Fuxetal.,2002),,,,(,2006).Sharon、Anammox25%,,,(Fuxetal.,2002),.Sharon-Anammox,、NH+4-N、NO-2-NNO-3-NSharon-Anammox,PCR-(DGGE),Sharon-Anammox.2 (Materialsandmethods)2.1 实验装置SBBR(1),3L,25%,,(30.0±0.5)℃;3,.SBBR、、4,.1 SBBR(1.;2.;3.;4.;5.;6.;7.;8.;9.;10.;11.)Fig.1 PlotoftheSBBRreactor(1.samplingpoint;2.effluentoutlet;3.temperaturecontrollayer;4.suspendedpadding;5.microporeaerator;6.rotorflowmeter;7.regulatevalve;8.aircompressor;9.timecontroller;10.watercyclepump;11.constanttemperaturewatertank)2.2 实验材料,,1.181011:SBBR1 Table1 Qualitiesofleachatemg·L-1CODBOD5NH+4-NNO-2-NNO-3-NSSpH894.2～2274.2536.4～1364.5376.2～427.30.23～0.390420.6～5117.57～7.95 ,,QTL-80H.2.3 运行参数及实验方法44SBBR(a、b、c、d),2,16h、12h、12h8h.,24h,,(30.0±0.5)℃(,2004;Thamdrup,2002),pH7.7±0.3,(Strousetal.,1997;,2006),Sharon1.0～1.5mg·L-1(,2004),,DO(1.2±0.1)mg·L-1.2 Fig.2 Timedistributivemapofoxygensupplyineachreactor4,:4,600mL,,;:600mL,10d200mL,,;:,,、,,,Sharon-Anammox;,,PCR-DGGE.2.4 分析方法COD:HB-Ⅱ;BOD5:HT99724A-6BOD5;NH+4-N:;NO-2-N:N-(1)-;SS:(,2002);pH:pHS-3TC;DO:.NH+4-N、NO-2-NNO-3-N,,LovibondET99730.2.5 微生物种群的分子生物学研究5,Na、Nb、Nc、NdN;K-CTAB(,2006;Yangetal.,2007)DNA;16SrDNA341F(5'-CCTACGGGAGGCAGCAG-3')(Yoshidaetal.,2005;Ishi,2001;Kjellerupetal.,2005)907R(5'-CCGTCAATTCCTTTGAGTTT-3')(Sangonetal.,)(Suzuki,2004;Hashizumeetal.,2005)DNAPCR,Bio-RadDcodeTMPCRDGGE,35%～65%,55℃,14h;0.5μg·mL-130min,20min,Bio-RadGelDoc2000,DGGE.3 (Experimentalresults)3.1 反应器内功能微生物的驯化与增殖,,、.4600mL,;、pH、DO,1811 27,,.,