搜索
李久义左华栾兆坤朱宝霞贾智萍(,,,100085).,,,.:,,,.(EPS),.EPS,EPS,.,EPS,EPS[1],EPS.,,,COD30%[2].EPS.111,55cm,20m,24mm.,1006mh-1.(1).,.A,B,COD.,,EPS.216200211ENVIRONMENTALCHEMISTRYVol.21,No.6November20021(20)Table1Thecompositionofconcentratedsyntheticwastewater/gl-1ABNaHCO31010(NH4)2SO4497497MgCl26H2O016016CaCl2002002KH2PO40001500015/gl-1ABK2HPO4004004FeCl36H2O0005000505060002530002428812EPS[3]EPS,:.10g20ml,3500rmin-110min,;:.20ml022%85%NaCl,300rmin-11min,;:.12000rmin-130min,;:EPS.022m,;:.,,.8000120002L,57h.EPS.EPS,,DNA,,DNA.[4],[4],DNADAPI[5].,EPS.21.21EPSEPS.1,EPS,100cm,,BEPS.,/(F/M),.,70cm,(TOC).,F/M,5476:,EPS[6,7].F/M,.,,,,EPS.,EPS.,A,,EPS;B,EPS.,,90%70cm.EPS,,.22EPS,,EPS,[810].,EPS,.,EPS(1),EPS,,,EPSF/M.23,100cmEPS,,,,(1).,,40cm90%.,106,EPS[11],.,[12].Daniellson[13],EPS,.,EPS,.,EPS.24DNAEPSDNA,.DNAEPS115%,1DNA.EPSDNA,EPSDNA,DNA,EPS.,DNA.548211Fig.1Theeffectofsubstrateconditionsoncompositionsofextracellularpolymericsubstancesinbiofilms25210cm.,,.,,;,,,.2,EPS.2Fig.2Surfacemorphologicalcharacteristicsofbiofilmstakenfrom10cmportofbothfiltersusingascanningelectronmicroscope.(leftfromfilterAandrightfromfilterB)5496:3(1),EPS.(2)EPS,EPS.(3)EPS,EPS,.(4)EPSDNA.(5).[1]ZhangXQ,BishopP,SpatialDistributionofExtracellularPolymericSubstancesinBiofilms.J.Env.Eng.,2001,127(9)850856[2]GuellilA,BlockJC,ThomasFetal.,TransferofOrganicMatterbetweenWastewaterandActivatedSludgeFlocs.Wat.Res.,2001,35143150[3]ZhangXQ,BishopP,KinkleBK,ComparisonofExtractionMethodsforQuantifyingExtracellularPolymersinBiofilms.Wat.Sci.Tech.,1999,39(7)211218[4],.,1994[5]BrunkCF,JonesKC,JamesTW,AssayforNanogramQuantitiesofDNAinCellularHomogenates.Analyt.Biochem.,1979,92497500[6]HarrisRH,MitchellR,InhibitionoftheFlocculatonofBacteriabyBiopolymers.Wat.Res.,1975,9993999[7]AndreadakisAD,PhysicalandChemicalPropertiesofActivatedSludgeFlocs.Wat.Res.,1993,2717071714[8]LiaoBQ,AllenDG,DroppoIGetal.,SurfacePropertiesofSludgeandTheirRoleinBioflocculationandSettleability.Wat.Res.,2001,35399350[9]AzeredoJ,LazarvoV,OliveiraR,MethodstoExtracttheExopolymericMatrixfromBiofilms:aComparativeStudy.Wat.Sci.Tech.,1999,39(7)243250[10]MartinCerecedaM,JorandF,GuinerAetal.,CharacterizationofExtracellularPolymericSubstancesinRotatingBiologicalContactorsandActivatedSludgeFlocs.Environ.Tech.,2001,22951959[11]JimenezB,NoyolaA,CapdevilleBetal.,DextranBlueColorantasaReliableTracerinSubmergedFilters.Wat.Res.,1998,2212531257[12]ConferDR,LoganBE,MolecularWeightDistributionofHydrolysisProductsduringBiodegradationofModelMacromoleculesinSuspendedandBiofilmCultures.:BovineSerumAlbumin.Wat.Res.,1997,3121272136[13]DignacMF,UrbainV,RybackiDetal.,ChemicalDescriptionofExtracellularPolymers:ImplicationonActivatedSludgeFlocMatrix.Wat.Sci.Tech.,1998,3845532002423.55021EFFECTSOFSUBSTRATECONDITIONSONCOMPOSITIONSOFEXTRACELLULARPOLYMERICSUBSTANCESINBIOFILMSLIJiuyiZUOHuaLUANZhaokunZHUBaoxiaJIAZhiping(StateKeyLaboratoryofEnvironmentalAquaticChemistry,ResearchCenterforEcoEnvironmentalSciences,theChineseAcademyofSciences,Beijing,100085)ABSTRACTTheeffectsofcolloidalandsolubleorganicmattersontheamountsandcompositionsofextracellularpolymericsubstances(EPS)inbiofilmswerestudiedinbenchscalebiofilters.TheresultsofexperimentsindicatedthattheamountsofcarbohydratesandproteininbiofilmEPS,aswellastotalEPS,weresignificantlyinfluencedbythedifferenceofsubstrateconditions.Thesurfacepropertiesofbiofilms,observedbyascanningelectronmicroscope,weretosomeextentaffectedbythesubstrateconditions.Keywords:biofilm,extracellularpolymericsubstancs,colloidalorganicmatter,solubleorganicmatter.5516: