不同类型及不同浓度抗生素条件下活性污泥丝状菌种群多样性分析王润芳1

36620156ENVIＲONMENTALSCIENCEVol．36No．6Jun．20151122*1．0710022.100085、、6．EikelboomJenkinsC/N．FISHNostocoidalimicolaN．limicolaⅡThiothrixⅡ．5mg·L－1、25mg·L－1CODNH+4-N20mg·L－1N．limicolaⅡThiothrixⅡ．．X172X703.1A0250-3301201506-2239-06DOI10．13227/j．hjkx．2015．06．0432015-01-092015-02-201989～[e-mail]*[e-mail]ntousBacteriaCommunityDiversityinActivatedSludgeUnderVariousKindsandConcentrationConditionsofAntibioticsWANGＲun-fang1WANGQin1ZHANGHong2QIＲong2*1．CollegeofChemistryandEnvironmentalScienceHebeiUniversityBaoding071002China2．ＲesearchCenterforEco-EnvironmentalSciencesChineseAcademyofSciencesBeijing100085ChinaAbstractThreekindsofsyntheticantibioticspiramycinoxytetracyclinestreptomycinwastewaterwerecontinuouslytreatedbyparallelaerobicbiofilmreactorsfor6monthsrespectively．Sludgebulkingphenomenoncausedbyovergrowthoffilamentousbacteriawasobservedunderlong-termhighrbCODandhighC/NconditionsinallreactorsasshowedbytheEikelboomandJenkinsexaminations．ThequalitativeanalysisoffilamentousbacterialpopulationinthebiofilmandsuspendedsludgeusingfluorescenceinsituhybridizationFISHshowedthatthedominatedfilamentousbacteriainthebioreactorwereN．limicolaⅡandThiothrixⅡ．Underconditionsofdifferentantibioticconcentrations5mg·L－125mg·L－1therewasnoobviouschangeintheCODremovalefficiencyoftheparallelreactorswhiletheNH+4-Nconcentrationabout20mg·L－1occurredunderhighstreptomycinconcentration．ThefilamentousbacteriaabundancewasreducedwiththeincreasingantibioticconcentrationespeciallysignificantforN．limicolaⅡ．TerramycinhadasignificantinhibitoryeffectonfilamentousbacteriapopulationfollowedbyStreptomycinandSpiramycin．Keywordsantibioticsactivatedsludgefilamentousbacteriapopulationabundanceinhibition、．1～9．filamentousbacteria10～18．EikelboomJenkins、、．3611.14A、B、C、D2LHＲT24hMLSS3000mg·L－1．、．、．1．ABCD．5mg·L－1Ⅰ100d25mg·L－1Ⅱ．2．11Table1Componentsandwaterqualityindexesofsyntheticantibioticwastewater/mg·L－1/mg·L－150COD400100NH+4-N301505a/25b1005a/25bNH42SO41265a/25bKH2PO4311mL·L－11aⅠbⅡ2Table2Compositionoftraceelementstocksolution/mg·L－1/mg·L－1H3BO350CuSO4·5H2O10MnSO4·4H2O40CoCl210ZnSO4·7H2O40KI10Na2MoO4·4H2O20NiCl2101.2COD、NH+4-N、NO－2-N、NO－3-N19．Eikelboom20FISH21．22.134CODCCODB、D．．NH+4-NNH+4-NI5mg·L－14NH+4-N96%Ⅱ25mg·L－1BNH+4-NC、DNH+4-NCNH+4-NDⅡNH+4-N4mg·L－1NH+4-N20mg·L－1．．3CODNH+4-NTable3CODandNH+4-Nremovalperformanceofdifferentreactors/mg·L－1/%ⅠⅡCODA40088．5090．50B40085．0088．25C40084．7584．50D40089．5090．75NH+4-NA3099．1799．23B3097．4098．30C3097．4094．93D3098．2383．472.2EikelboomJenkins、145．Type0041Type00920～1．4、．Ⅰ90d5mg·L－1．404226100～200μm．A0～13B、D3～4C1～2．4D．4．C．Ⅱ180d25mg·L－1．ⅠAB、D2C1～2．AB、C、D200μm．A、B、CD．4．AB、DⅠ．1100×Fig．1Changesinabundanceoffilamentousbacteria4Table4SludgecharacteristicsofbiofilmatdifferentstagesABCDⅠⅡⅠⅡⅠⅡ44421143/μm≥150≥200100～200≥20050～150≥30050～200≥2005Table5CharacteristicsofsuspendedsludgeindifferentreactABCDⅠⅡⅠⅡⅠⅡ154531153/μm≥100≥150≥200≤150100～20050～150100～300100～200100～3002.3EikelboomFISH423．34Type0041、N．limicolaⅡType021N1．AⅠN．limicolaⅡThiothrixⅡⅡ1Ⅰ43Ⅱ．22CODN．limicolaⅡ142236a1000×ThiothrixⅡ、N．limicolaⅡ、Sphaerotilusnatansb1000×ThiothrixⅡ、N．limicolaⅡ、SphaerotilusnatanscFISH1000×ThiothrixⅡGAM42a5'-cy3、N．limicolaⅡALF9685'-cy3、SphaerotilusnatansBET42a5'-cy32Fig．2Identificationofdominantfilamentousbacteriainreactors3Fig．3AbundanceoffilamentousbacteriainbiofilmsofdifferentreactorsThiothrixⅡNThiothrixⅡ．COD1∶4C/N13.3/14Fig．4Abundanceoffilamentousbacteriainsuspendedsludgeofdifferentreactors24226．AⅠⅡThiothrixⅡ5．B、C、D．B、DN．limicolaⅡThiothrixⅡⅠ3A．BⅡ2DN．limicolaⅡ1ThiothrixⅡ2．B、DAⅠThiothrixⅡ5Ⅱ32．CⅠⅡN．limicolaⅡ1ⅠN．limicolaⅡThiothrixⅡ1ⅡThiothrixⅡ1．B、．N．limicolaⅡThiothrixⅡAN．limicolaⅡThiothrixⅡ．DB．C．ⅠⅡ．C/NN．limicolaⅡThiothrixⅡ．、1mg·L－123250μg·L－124190μg·L－125．A3．HＲTSＲTNH+4-N．31．2、、N．limicolaⅡ、ThiothrixⅡ＞＞．35mg·L－114．1．/-MBＲD．2014．43-48．2．J．2010304504-509．3MartinezAGSanchezAＲToledoMVMetal．Effectofciprofloxacinantibioticonthepartial-nitritationprocessandbacterialcommunitystructureofasubmergedbiofilterJ．ScienceoftheTotalEnvironment2014476-477276-287．4ZhangYXieJPLiuMMetal．MicrobialcommunityfunctionalstructureinresponsetoantibioticsinpharmaceuticalwastewatertreatmentsystemsJ．WaterＲesearch201347166298-6308．5MatosMPereiraMAParpotPetal．InfluenceoftetracyclineonthemicrobialcommunitycompositionandactivityofnitrifyingbiofilmsJ．Chemosphere2014117295-302．6FatoneFBattistoniPPavanPetal．Operationandmaintenanceoffull-scalemunicipalmembranebiologicalreactorsAdetailedoverviewonacasestudyJ．Industrial＆EngineeringChemistryＲesearch200746216688-6695．3422367MartinsAMPPagillaKHeijnenJJetal．Filamentousbulkingsludge—acriticalreviewJ．WaterＲesearch2004384793-817．8．J．2004101-4．9．J．2004201233-36．10．J．20143562241-2248．11．J．201228315-19．12．J．20113761-5．13．MBＲJ．2009301155-159．14．J．200925346-49．15．J．200723964-66．16．-J．2006386887-892．17．MBＲJ．200425S156-58．18．J．20001625-8．19．M．．2003．20EikelboomDH．FilamentousorganismsobservedinactivatedsludgeJ．WaterＲesearch197594365-388．21NielsenPHDaimsHLemmerH．FISHhandbookforbiologicalwastewatertreatmentidentificationandqu