不同类型人工湿地微生物群落的研究进展周元清

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*121**211112000622653100、。、、。DGGEFISH、、、、-、。。Q93A1000-489020116-1251-07Areviewonsoilmicrobialcommunityindifferenttypeconstructedwetlands.ZHOUYuan-qing12LIXiu-zhen1**LIShu-ying2TANGYing-ying1XINZai-jun1JIAYue11StateKeyLaboratoryofEstuarine&CoastalResearchEastChinaNormalUniversityShanghai200062China2CollegeofEnvironmentalResourcesofYuxiNormalUniversityYuxi653100YunnanChina.ChineseJournalofEcology20113061251-1257.AbstractAsanindispensablepartofconstructedwetlandsoilmicrobesplayanimportantroleinthematerialtransformationandenergyflowinthewetlandecosystem.Thispaperreviewedtheresearchprogressinthedetectionmethodsofsoilmicrobialcommunityitsstructureandcomposi-tionandrelationshipsofsoilmicrobialregulationeffectwithenvironmentalparametersindifferenttypeconstructedwetlands.VariouskindsofnovelmolecularbiologicalmethodshaveprovedtobeusefultoolsforexploringsoilmicrobialdiversityinconstructedwetlandamongwhichdenaturinggradientgelelectrophoresisDGGEandfluorescenceinsituhybridizationFISHwerethemostfrequentlyappliedmethods.Theregulationeffectofsoilmicrobialcommunityinconstructedwet-landmainlydependedonwetlandhydraulicconditionwastewaterpropertyconstituentcomposi-tionandpollutantscharacteristicsandavailabilityfiltermaterialorsoiltypeplantandvari-ousenvironmentalfactors.Thespecies-specificcompositionofsoilmicrobialcommunityindiffer-enttypeconstructedwetlandswasdominatedbyphylumProteobacteriafollowedbythemembersofphylumsProteobacteriaCytophaga-FlavobacteriumActinobacteriaandFirmicutes.Howtofurtherimprovetheresearchesonthediversityofsoilmicrobesinvolvinginnitrogencycleandtoincreasetheremovalefficiencyofnitrogenfromwastewaterwouldbeoneofthekeyissuesforfur-therdevelopmentofconstructedwetlandtechnology.Keywordsmicrobialcommunitymicrobialactivitymicrobialbiomassconstructedwetland.*20100470768、2010RCDW082008KYYW072008ZC161M。**E-mailLandscape2001@sina.com2010-11-222011-02-28ChineseJournalofEcology20113061251-1257DOI:10.13292/j.1000-4890.2011.0222、Matamorosetal.2007Schroderetal.2007。surfaceflowconstructedwetlandsSFCW、horizontalsubsurfaceflowconstructedwetlandsHSS-FCWverticallysubsurfaceflowconstructedwetlandsVSSFCW。SFCW0.4mKadlec&Knight1996Scholz&Lee2005。HSSFCWVSSFCW。Vymazal20052008、3。Liikanenetal.2004Manderetal.2005。、3Sundbergetal.2007b。、Nurketal.2005Truuetal.2005Nicomratetal.2006Sundbergetal.2007aTaoetal.2007bTietzetal.2007。、、。、、。1。。。Doran&Zeiss2000。Dilly2004、、、。、、、、、、、、Schloteretal.2003Bendingetal.2004Truuetal.2008。Kangetal.1998Nguyen2000Shackleetal.2000Nurketal.2005Tietzetal.2007。Tietz2007SIR、ATP。。ReddyDangelo1997。。。、、pH、、。SOD。2521306、、Reddy&Dangelo1997Liangetal.2003。themost-probable-numberMPNMPN。1%~10%。TorsvikOvreas2002。。rRNA2001。16SrRNA。SSCP、RFLP、DNARAPD、AFLP、DGGETGGEFISHDGGEFISH。DGGEDNATMDNADNADNA。DNA。。。Muzyersetal.1993。Truu2005DGGEHSSFCW。HSSFCW。FISHDNADNA。、2007。Flood1999Shipin2005FISHSFCWVSSFCWα-β-γ-18%~41%。HSSFCW。、、、。2HSSFCW、VSSFCWSFC-Ws。Tietz2007VSSFCW0~10cm、10~20cm20cm。Sleytr200710~20cm。Nguyen2000Nurk2005HSSF-CW。Kern2003。C/N2Nurketal.2005。3521Nicomrat20062008DGGERFLP。Ibekwe20032Acidithiobacillusfer-rooxidansAcidithiobacillusthiooxi-dans。DGGEHSSFCWBacillus、Clostridium、Mycoplasma、EubacteriumPro-teobacteria。Ahn200716SrRNALH-PCR。α-40%~60%ActinobacteriaFirmicutes。DNAWalshetal.2002Lloydetal.2004。Ibekwe20073136operationaltaxonomicunitsOTUs。Walsh200237OTUsIbekwe20072。YuMohn2001RFLPPCR16SrRNA。DO。56OTUsCytophagales。CriadoBecares2005SFCWHSSFCWα-1.9%Shipin200510.5%-Cytophaga-Flavobacterium。Baptista2008FISHDGGEHSSFCW、。。Ahn2007。Ibekwe2007SFCW50%100%。2004BOD5。、-、Yu&Mohn2001Shipinetal.2005Ahnetal.2007。31994DuncanGroffman1994。2005。ScholzLee2005、、。2006。。Galeetal.1993Kern2003。Ko-zub&Liehr1999Shackleetal.2000Taoetal.2007a。2008。4521306Kozub&Liehr1999Sirivedhin&Gray2006Paredesetal.2007。COD。Tanner2002CODHSSFCW。。Zhou2005VSSFCW、β-。NH4+-NParedesetal.2007Sirivedhin&Gray2006。Reddy&Dangelo1997Tanneretal.2002Paredesetal.2007。Braeckevelt2007。Sund-berg2007b。Baptista2003。pH。pH7.0~8.0Paredesetal.2007。20103pH。pH7.0Taoetal.2007b。。5℃4℃Sirivedhin&Gray2006。Manderetal.2005。>15℃25℃Paredesetal.2007。Kern2003HSSFCWCO2。、、。4。HSSFCW-Duseketal.2008。。。。DGGEFISHGilbrideetal.2006Nakatsu2007Nockeretal.2007。。。、、。、。Konnekeetal.2005。。.2005..18644-49..2001.rRNA5521.21137-43..2004..164312-31..2008..30675-77..2006..262225-229..2008..2762401-2406..2010..342256-261..2007..26457-60.AhnCGillevetPMSikaroodiM.2007.Molecularcharacter-izationofmicrobialcommunitiesintreatmentmicrocosmwetlandsasinfluencedbymacrophytesandphosphorusloading.EcologicalIndicators7852-863.BaptistaJDDavenportRJDonnellyTetal.2008.Themi-crobialdiversityoflaboratory-scalewetlandsappearstoberandomlyassembled.WaterResearch423182-3190.BaptistaJDCDonnellyTRayneDetal.2003.Microbialmechanismsofcarbonremovalinsubsurfaceflowwetlands.WaterScienceandTechnology48127-134.BendingGDTurnerMKRaynsFetal.2004.Microbialandbiochemicalsoilqualityindicatorsandtheirpotentialfordifferentiatingareasundercontrastingagriculturalmanage-mentregimes.SoilBiology&Biochemistry361785-1792.BraeckeveltMRokadiaHImfeldGetal.2007.Assessmentofinsitubiodegra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