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2003,23(3)267~271ChinaEnvironmentalScience,(,210096),.,,.,(HRT)2h,85.9%84.0%,-LRRR-LRRR86.7%81.7%71.5%80.5%..X52A10006923(2003)03026705Degradationcharacterofmicrocystininbiofilmreactorswithdifferentflowpatterns.ZHUGuang-can,LUXi-wu(DepartmentofEnvironmentalEngineering,SoutheastUniversity,Nanjing210096,China).ChinaEnvironmentalScience,2003,23(3)267~271AbstractTheremovalcharacterwasexploredinthree-stepbiofilmreactor,one-stepbiofilmreactorwiththesamedirectionsofwaterflowandairflow,andone-stepbiofilmreactorwiththeoppositedirectionsofwaterflowandairflowwiththeeutrophicsourcewaterastreatmentobject.Three-stepreactorwasclosertoplugflowreactorandhadhigherandstablerremovalefficiencyofmicrocystinthanone-stepones.Undertheconditionsofwaterqualityintheexperiment,whenHRTwas2h,85.9%ofextracellularmicrocystinand84.0%oftotalmicrocystinwereremovedinthree-stepreactor.Meanwhile,theremovalratesofextracellularmicrocystin-LR,RRandtotalmicrocystin-LR,RRwere86.7%,81.7%,71.5%and80.5%respectively.Three-stepbiofilmtechnologycouldbeusedforpretreatmentofeutrophicsourcewater.Keywordsmicrocystinbiofilmreactorbiologicalpretreatmentexit-agedistributionfunction,,,[1].,25%~70%[2].7,(Anabaena)(Aphanizomenon)(Microcystis)[3].,[4].,[5,6],1.0µg/L.[7,8],,,.()()3,,.11.1.(200161~1030)NH3N0.5~8.2mg/L,CODMn6~12.6mg/L,106~108/L.20020913(59978007);(BS2000012);(XJ000509)*268231.21.2.11,,,4.47L.,.,..,1.4~8mg/L.1Fig.1Schematicdiagramofthree-stepbiofilmreactor1.2.3.4.()1.2.2,,.,7.6mmol/L,2mL,90min.,E(t)[9],E(t),E(t)~t.1.3(ELISA)(MC)(MCMC,MC),(HPLC)-LRYRRR(MCLRMCYRMCRRMCLRMCYRMCRR).1.3.120MHz2h,,MC.MC,.0.45µmwhatmanGF/C,.1.3.2ELISA96,;S/N5810Vmaxkineticmicroplatereader(MolecularDevices);KS-250().3,3.2,15min,,450nm.,,MC.1.3.3HPLCMCLRYRRRC49H74N10O52C52H52N10O13C49H75N13O12,;();H3PO4();NaH2PO4();Waters2690,Waters2487;BDSC18(4.6mm×250mm,5µm)C18C18ODS(Sep-pakCartridge);0.45µmwhatmanGF/C;501();JHN-2A().C185mL220mL2.MC,5mL/minC18,10%C182,2mL2,40,1mL.,,.,58%CH3OH,37%0.05mol/LH3PO4,5%0.05mol/LNaH2PO4;1mL/min;239nm;10µL;40.1.4;NH3N;CODMn.2431326922.1(HRT)MCMC0.23~1.45µg/L,MC0.65~2.60µg/L.MCYRHPLC.,,MCHRT,HRT2h,.MC,,MCMC.HRT2h,MC50.0%63.2%,MC83.4%83.1%.,MC,MC.,.1Table1RemovalofalgaeindifferentreactorsHRT=1.5hHRT=2hHRT=3h(106/L)η(%)(106/L)η(%)(106/L)η(%)24.075.756.39.7959.28.7588.49.0983.96.2374.09.4887.58.6884.64.9079.66.1091.95.7589.86.0075.01.0985.65.6690.08.4664.81.0985.66.7688.0:,,HRT2h,MCMC85.9%84.0%(2),MCLRMCLR86.7%71.5%(3),MCRRMCRR81.7%80.5%(4),91.9%.MC76.5%16.8%6.7%,MC79.9%,17.2%,2.9%,MC,MC,MC.2MCFig.2DegradationofMCindifferentreactors´3MCLRFig.3DegradationofMCLRindifferentreactors22.25,340506070809010012345HRT(h)MC(%)02040608010012345MC(%)2040608010012345HRT(h)MCLR(%)02040608010012345MCLR(%)27023HRT97,95,102min,HRT(90min).,.,,HRT,[9],2~4.,HRTHRT,.4MCRRFig.4DegradationofMCRRindifferentreactors25Fig.5Exit-agedistributionfunctionofthreereactors2.3MC,[10],MCAdda,[11,12].JonesOrr[11]CentenaryMCLR.MCLR9d,,3d1800µg/L100µg/L,9d.Jones[13]1000µg/LMCLR23d,5%.,10~50µg/LMCLR.[14]MC,5~7dMC.,MC.,,MC(2~4),MC0.5µg/L.Jones[13]MCLR(100µg/L),MCLR,MCLR,MCLR,.MCCODMn,MC,.,MC.[15],,,.,.,94%NH3N85.9%MC84.0%MC,,,.304560759012345HRT(h)MCRR(%)02040608010012345MCRR(%)05101520253004080120160200(min)E(t)(103)327133.1,MC.3.2,,HRT2h,90%,MCMCLRMCRR85.9%86.7%81.7%,MCMCLRMCRR84.0%71.5%80.5%.3.3.[1].[J].,1992,12(3):204208.[2]DuyTN,LamPKS,ShawGR,etal.Toxicologyandriskassessmentoffreshwatercyanobacterial(blue-greenalgae)toxinsinwater[J].Rev.Environ.Contam.Toxicol.,2000,163:113186.[3]CarmichaelWW.Hemagglutinationmethodfordetectionoffreshwatercyanobacteria(blue-greenalgae)toxins[J].AppliedandEnvironmentalMicrobiology,1981,41(6):13831388.[4]Nishiwaki-MatsushimaR,OhtaT,NishiwakiS,etal.Livertumorpromotionbythecyanobacterialcyclicpeptidetoxinmicrocystin-LR[J].JCancerRes.Clin.Oncol.,1992,118:420424.[5]LambertTW,HolmesCFB,HrudeySE.Microcystinclassoftoxins:healtheffectsandsafetyofdrinkingwatersupplies[J].Environ.Rev.,1994,2:167186.[6],,,.[J].,1998,15(3):111113.[7]KeijolaAM,HimbergK,EsalaAL,etal.Removalofcyanobacterialtoxinsinwatertreatmentprocesses:laboratoryandpilot-scaleexperiments[J].ToxicityAssessment,1988,3:643656.[8]RositanoJ,NicholsonB,PieronneP.Destructionofcyanobacterialtoxinsbyozone[J].OzoneScienceandEngineering,1998,20(3):223238.[9],.[M].2.:,2000.[10]TsujiKT,WatanukiT.Stabilityofmicrocystinsfromcyanobacteria-IV.Effectofchlorinationondecomposition[J].Toxicon,1997,35(7):10331041.[11]JonesGJ,OrrPT.Releaseanddegradationofmicrocystinfollowingalgaecidetreatmentofamicrocystisaeruginosabloominarecreationallake,asdeterminedbyHPLCandproteinphosphataseinhibitionassay[J].Wat.Res.,1994,28(4):871876.[12]CousinsIT,BealingDJ,JamesHA,etal.Biodegradationofmicrocystin-LRbyindigenousmixedbacterialpopulations[J].Wat.Res.,1996,30(2):481485.[13]JonesGJ,BourneDG,RobertL,etal.Degradationofthecyanobacterialhepatotoxinmicrocystinbyaquaticbacteria[J].NaturalToxins,1994,2:228235.[14],,,.[J].,2002,22(2):189192.[15],,.[J].,1999,19(2):138140.(1972),,,,,.8.,.PingChen(Nature2002,420,302-304)(Li3N),1Li3N3.6.,275(),.,.Li3N,30bar