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＊贾振杰 李慧君 杨清香＊＊ 张 昊 陈建军( 453007):RB5M-3BE,、A、BD。11,5D,AB3。A3,50mgLM-3BEA99.53%97.42%,16,、()、;BD,32,。A、B,,D。:,,,:Q939.99 :A :0253-2654(2007)04-0629-04EffectsofDifferentMediaonEnrichingandScreeningFungiCulturewiththeAbilitiesofDecolorizingVariousSyntheticDyes＊JIAZhen-Jie LIHui-Jun YANGQing-Xiang＊＊ ZHANGHao CHENJian-Jun(CollegeofLifeSciences,HenanNormalUniversity,KeyLaboratoryofEnuironmentalPollutionControlTechnologyofHenanProvince,Xinxiang 453007)Abstract:Inthispaper3differentmedia(A,foryeastcultivation;B,forlaccaseproducing;D,forwhiterotfungicultivation)werecomparedinenrichingandscreeningdecolorizingfungicultureusingReactiveBlack5(RB5)andReactiveRed(M-3BE)fromthefollowingthreepoints:decolorizationeffects,abilitiesofproducingenzymesanddiversityofmicrobialcommunity.11groupsoffungiwithobviousdecolorizationeffectswereobtainedafterenrichmentfornearonemonth.Amongthem,6groupscamefrommediumD,theothertwo3groupsfrommediumAandB,respectively.However,the3groupsfrommediumAexhibitedthehighestmicrobialdiversityandbestdecolorizationresultswith99.53%and97.42%colorremovalrateofReactiveRedM-3BEandAcidRed.Fromthem,16strainsoffungiwereisolatedandprimarilyidentifiedasSaprolegniaceae,Eurotiaceae(Monascuswent),ErysiphaceaeandPhysodermataceae.FungigroupsfrommediumBandDexhibitedabitlowercolorremovalrateofvariousdyesandonly3and2isolatesprimarilyclassifiedasSaccharomycetaceaeandEurotiaceae(Penicillium)wereobtainedfromthem.FungiculturesinmediumAandBcouldproduceligninperoxidase,andthoseinmediumDcouldbedetectedhigheractivityoflaccase.Allthefungalculturesexhibitedveryweakactivityofmanganesedependantperoxidase.Keywords:Fungi,Decolorizationofdyes,Medium,Enzyme ＊“”(No.NCET-05-0612);(No.20677014) ＊＊ Tel:0373-3326703, E-mail:yangqx66@163.com:2006-09-27,:2006-12-18 ,,。,,[1]。,、、、,,[2]。,,·629· 200734(4)DOI:10.13344/j.microbiol.china.2007.04.036,[3]。,。,,,,[2～4]。。,,3。1 1.1 A[5]。B:4.5g,1.5g,1g,NH4Cl0.5g,100mL。:KH2PO42g,MgSO4·7H2O0.5g,CaCl20.1g,KCl0.5g,1LpH5.0。D[6]。1.2 、、、。1.3 RB5(λm=596nm),M-3BE(λm=541nm),(λm=516nm),KNR(λm=600nm),10mgmL,4℃。1.4 1g100mL3,28℃、140rmin,5d～7d。1h,。50mgLRB5、M-3BE5d～7d,100mgL,。1.5 4,50mgL,28℃、140rmin,[7]。。=(A0-At)A0×100%A0、AttOD。1.6 5000rmin10min,。(LiP)Tien&Kirk[8]。min1μmol。[9]。240nmmin0.1OD。ABTS[10],-,420nm3min,min0.1OD。1.7 ,,28℃,3d,,,。2 2.1 432(M-3BERB5),30。,AM-3BE3,A1R、A2R、A5R,RB5;BB1R、B3R,RB5B6B;DD1R、D4R、D5RRB5D6BD7B。1。,AM-3BEBD,A45。,3,,96h。5·630·200734(4),,48h,,96h20%。2.2 1150mgLAKNR,,24h,。1324hAKNR。1 (24h%)ABDA1RA2RA5RB1RB3RB6BD1RD4RD5RD6BD7BRB559.768.774.1M-3BE99.598.880.378.039.268.861.656.81 24hAKNRA,BKNR 1,11A,A1R,A100%。KNR,B(B1R、B5R、B6B)。A、BD3KNR59.7%～70%、77%～92.3%36.1%～62.9%。2.3 11,115,2。2,A,,;B;D,、D1R、D4RD6B。,,AM-3BE,AM-3BE。KNR,A2R、A3R、D1R、D5R,,59.68%～69.93%。BKNR,,213UL～253UL,KNR(1),。KNR,,100mg30000U,50℃,4h,12h,70%[11],,KNR,24h,B6B92.32%。2.4 ,·631· 200734(4)3。A16,、()、,BD5,。,A,BD。A,。2 3(UmL)M-3BEAKNRLiPLacMnPLiPLacMnPLiPLacMnPAA1R17--210--70--A2R20--18023--1055-A5R--907210-901910-BB1R27--60--253--B3R--10---213--B6B---24060-22727-DD1R----16--2787-D4R---633307--90-D5R223--------D6R----10--3940D7R--30--40--1003:LiP,MnP,lac,- 218。。[1]SafiaM,XamaK,DattaM.DyesandPigments,2007,74(3):723～729.[2]KapdanI,KargiF,McMullanG,etal.BioprocessEngineering,2000,22:347～351.[3]HeF,HuW,LiY.Chemosphere,2004,57:293～301.[4]ChenB,ChenS,LiM,ChangJ,ProcessBiochemistry,2006,41:1574～1581.[5]YangQ,YedilerA,YangM,etal,Biochemicalengineeringjournal,2005,24:249～253.[6],,.1999,13(2):135～140.[7],,.2006,32(3):52～55.[8]TienM,KirkTK.MethodsinEnzymology,1988,161:238～249.[9],,.,2004,19(2):29～32.[10], , .(),2003,2(1):83～90.[11],, ,.,2004,24(1):48～52.[12],,,2005,11(6):763～766.论文中有关正、斜体的约定:、(、)。,,。,。:,,:BamHI、EcoRI、MspI、Sau3AI。:3,,,。。,,。·632·200734(4)