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20114717·NutritionandFeedstuffs———————————————————————————2010-10-212011-01-14、-nycytx-381982-*50225。、。、P0.05P0.05、、P0.05。S823.5B0258-7033201117-0034-0412111*1.1301182.121001、、。2。。。、。、、。11.1、、50225。1.22、12。30d2d3d。1.32。04:00、16:00TMR0.5h。1。1.430min05:0017:0013d100g。-20℃4℃。3420114717NutritionandFeedstuffs·/（·d-1）/%/%/%/%/%/%/%0.313.9427.7329.5451.8934.760.000.000.274.1226.5628.3143.4230.870.080.1612/%60.0060.0010.0010.0017.5517.556.006.001.051.052.202.201.001.001.201.201.001.00100100RND/（·d-1）5.204.94/（g·d-1）878.74888.64/（（g·d-1）44.7449.12/（g·d-1）26.4435.24245∶551.5、4N—AIA、、—0.05NHCl—0.025NH2SO4[2]、[3-4]28℃3。1.5.110-1、10-2…10-9。1.5.2。0.05mL。3。1.5.3《》、/mL/d10-6～-10-100.052～3-110-4～10-80.054～5100mg/kgPDA10-4～10-80.053～41%，0.3%3《》、《》。1.6ExcelSPSS16.0。22.142P＞0.05。4%12.77±2.111.97±1.91.419±0.131.387±0.112.252、P0.05、。5%DM62.45±2.2a57.67±1.7bPr63.85±2.1a59.03±1.8bP0.053520114717·NutritionandFeedstuffs2.3、6。P0.05。/（g·kg-1）11.4±0.81a15.3±1.20b/（mg·kg-1）1.38±0.63a2.91±0.82b/（mg·kg-1）5.8±0.7a8.2±0.43b/（mg·kg-1）0.37±0.54a0.61±0.68b6、P0.05。462.47、P0.05P0.05。2。722.43±0.51a16.61±0.32b8.97±0.31a6.68±0.12b8.75±0.34a6.41±0.24b4.62±0.633.65±0.4033.1、、、。NDF。Reese[5]。3.2。[6-7]。。。。。3.3。11255t1419t。。。。。Wu[9]。3.4。VanVLiet[10]、、8。3620114717·AnimalScience&Biotechnology7。[11]。。[1],.[J].,2005,36(10):643-647.[2],,,.[J].,2007,5(1):20-26.[3]RinsumJV,DijkWV,HooghwinkelGJ,etal.Subcellularlo－calizationandtissuedistributionofsialicacid-formingenzymesN-Acetylneuraminate-9-phosphatesynthaseandN-acetylneu－raminate9-phosphatase[J].BiochemJ,1984,223:323-328.[4]HaoJ,BalagurumoorthyP,SarillaS,etal.Cloning,expression,andcharacterizationofsialicacidsynthases[J].BiochemBiophysResCommun,2005,338(3):1507-1514.[5]NakataD,CloseBE,ColleyKJ,etal.MolecularcloningandexpressionofthemouseN-acetylneuraminicacid9-phosphatesynthasewhichdoesnothavedeaminoneuraminicacid(KDN)9-phosphatesynthaseactivity[J].BiochemBiophRes,2000,273(2):642-648.[6]RosemanS,JourdianGW,WatsonD,etal.Enzymaticsynthesisofsialicacid9-phosphates[J].ProcNatlAcadSciUSA,1961,47:958-961.[7]WarrenL,FelsenfeldH.N-acetylmannosamine6-phosphateandN-acetylneuraminicacid9-phosphateasintermediatesinsialicacidbiosynthesis[J].BiochemBiophResCo,1961,5:185-190.[8]LawrenceSM,HuddlestonKA,PittsLR,etal.CloningandexpressionofthehumanN-acetylneuraminicacidphosphatesyn－thasegenewith2-keto-3-deoxy-D-glycero-D-galacto-nonon－icacidbiosyntheticability[J].JBiolChem,2000,275(23):17869-17877.[9]HamadaT,ItoY,AbeT,etal.Solutionstructureofthean－tifreeze-likedomainofhumansialicacidsynthase[J].ProteinSci,2006,15:1010-1016.[10]HaoC,AstridB,MartinZK,etal.Purificationandcharacteriza－tionofN-acetyneuraminicacid-9-phosphatesynthasefromratliver[J].Glycobiology,2002,12(2):65-71.[11]AngataT,NakataD,MatsudaT,etal.BiosynthesisofKDN(2-keto-3-deoxy-D-glycero-D-galacto-nononicacid).Identifica－tionandCharacterizationofaKDN-9-phosphateSynthetaseAc－tivityfromTroutTestis[J].JBiolChem,1999,274:22949-22956.。、。4、,P0.05。,P0.05。。[1].[M].:,1980.[2].[M].:,1988.[3]BbernalMP,ParedesC,Sanchez-MonederoMA.Maturityandstabilityparametersofcompostspreparedwithawideoforganicwastes[J].BioresourceTechnol,1998,63:91-99.[4]Beck-FriisB.SmartS.Gaseousemissionscarbondioxide,ammoniaandnitrousoxidefromorganichouseholdwasteinacompostreactorunderdifferenttemperatureregimes[J].AgricEngnyRes,2001,78(4):423-430.[5]ReeseDE.Dietaryfiberinsowgestationdietsreviewed[J].NebraskaSwineReport,1997,11-25.[6]WeissWP,WillettLB,St-pierreNR,etal.Varyingforagetype,metabolizableproteinconcentration,andcarbohydratesourceaffectsmanureexcretion,manureammonia,andnitrogenmetabolismofdairycows[J].JDairySci,2009,92:5607-5619.[7]BiermanS,EricksonGE,KlopfensteinTJ,etal.Evaluationofnitrogenandorganicmatterbalanceinthefeedlotasaffectedbylevelandsourceofdietaryfiber[J].JAnimSci,1999,7:1645-1653.[8].[J].2001,22(1):48-51.[9]WuZUtilizationofphosphorusinlactatingcowsfedvaryingamountsofphosphorusandsourcesoffiber[J].JDairySci,2005,88:2850-2859.[10]VanVlietPCJ,ReijsJWBloemJ,etal.Effectsofcowdietonthemicrobialcommunityandorganicmatterandnitrogenvontentoffeces[J].JDairySci,2007,90:5146-5158.!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!3646