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、。、、、[1]。、[2]、、、、、、pH[3-4]。C/N[5]。、[6-7]、pH、[8-10]。2010,29(6):1187-1192JournalofAgro-EnvironmentScienceC/N、、4。C/N4C/NC/N1∶13∶150cm2∶124h80cm·d-1C/N5∶125cm1∶124h80cm·d-1。X703.1A41112341．5106422．DepartmentofWaterResourcesSt．JohnsRiverWaterManagementDistrictPalatkaFlorida32178,USA3．5106424．200001Vertical-flowConstructedWetlandDe-nitrificationImpactofFourControllableFactorswithDifferentC/NZHUWen-ling1,ZHENLi-ni1,CUILi-hua1,OUYANGYing2,LUOShi-ming3,TANGZhong-en41.DepartmentofEnvironmentalScienceandEngineering,SouthChinaAgriculturalUniversity,Guangzhou510642,China;2.DepartmentofWaterResources,St.JohnsRiverWaterManagementDistrict,Palatka,Florida32178,USA;3.CollegeofAgronomy,SouthChinaAgriculturalUniversity,Guangzhou,510642,China;4.ESDChinaLimited,Shanghai200001,ChinaAbstractThisstudyinvestigatedtheimpactsoffourdifferentcontrollablefactors,includinginflowrate,waterlevel,wet-to-dryratioandop－erationalcycle,upontheremovalofnitrogenfromthevertical-flowconstructedwetlandsundervaryingcarbon-nitrogenC/Nratios,usingtheorthogonalexperimentaldesign.ResultsshowedthatremovalefficiencyoftotalnitrogenTNincreasedwithC/N.Thefourdifferentcon－trollablefactorshadsignificantimpactsontheTNremovalandtheinflowratewasthemostimportantfactor.Theimpactsofthewet-to-dryratioincreasedwiththeC/Nratio.WhentheC/Nratioswere1∶1and3∶1,theoptimalcombinationforbestremovalofTNwaswaterlevelof50cm,wet-to-dryratioof2∶1,operationalcycleof24hoursandinflowrateof80cm·d-1.WhentheC/Nratiowas5∶1,theoptimalcombinationforbestremovalofTNwaswaterlevelof25cm,wet-to-dryratioof1∶1,operationalcycleof24hoursandinflowrateof80cm·d-1.Keywordsvertical-flowconstructedwetland;carbon-nitrogenratio;controllablefactors;totalnitrogenremoval2009－12－184087111030828005308280051983—。E-mailwlzhu701@163.comE-maillihcui@scau.edu.cn20106。、、4、。11.1PVC27100cm20cm90cm1～3cm10cm0.5~2mm80cm1。PVC5L、。1.21CODCr340、120、200mg·L-1。C/N1∶1、3∶1、5∶1。220、40、80cm·d-1。80cm·d-1。[11]33.53、7.06、14.12L·d-1。3N[12][13]2∶1、1∶1、1∶23。4、、31m90cm。25、50、75cm。512、24、48h3。1.331∶1、3∶1、5∶1、、、443。L93493。1。20095102009930。1.4。、1Figure1Schematicdiagramoftheexperimentalcolumn20cm10cm100cm80cm10cm143Table1The4-leveland3-factortableA/cmB∶C/hD/cm·d-11252∶112202251∶124403251∶248804502∶124805501∶148206501∶212407752∶148408751∶112809751∶2242041188296、、、。TN49.47~52.79mg·L-1。1.5TN[14]。SAS9.0SPSS16.0。22.13922。9C/N。。TN、[15][16]。C/N。。C/N。C/N1∶11、6、7、8、93、4P0.05。3、4C/N。2.23T1、T2、T3R。C/N。C/N1∶13∶150cm2∶124h14.12L·d-1。C/N5∶125cm1∶124h14.12L·d-1。4C/N1∶1、C/N2mg·L-1Table2Theeffluentconcentrationoftotalnitrogenmg·L-1±DuncanP=0.05。C/N=1∶1C/N=3∶1C/N=5∶111531.51±1.68ab31.83±0.93b31.33±0.67a21529.79±1.08bc29.94±0.89c21.99±1.34c31529.52±1.27c26.4±1.4e25.42±0.67bc41524.81±2.38d22.88±1.44f23.9±0.57c51532.66±1.08a31.69±1.42b31.48±0.91a61532.08±1.65abc32.18±1.36a31.63±0.32a71531.96±2.75ab29.14±1.08cd27.65±0.86b81531.76±1.07abc27.89±0.55de22.36±0.9c91532.87±1.15a29.45±1.06cd32.54±1.2a3Table3OrthogonalvisualanalysisofTNremovalrate123C/N1∶1、3∶1、5∶1。123123123123T117.2422.5032.9721.2526.3029.1913.8419.2327.2313.1218.1619.12T221.9223.8625.6616.7921.1835.7522.1227.6033.3616.8119.8030.61T314.5523.8329.9415.6722.7223.6317.7523.3727.9723.7832.2338.84R7.371.517.315.585.1312.138.289.926.1210.6614.0619.721189201063∶1C/N5∶1。2.32009101124。C/N5∶152.17%。C/N244.18%。C/N1∶13∶14C/N4。33.1C/NC/N。C/NBaker[17]1998C∶N5∶1。。[18]。C/N5∶1。。3.220～80cm·d-1。4C/N1∶11∶3。C/N[11]。[19]TN28.8~86.4L·d-1、。3.3C/N。C/N=5∶12、。2∶1、1∶1、1∶23。2∶1C/N1∶1、3∶1C/N5∶11∶1[12]。、。C/N5∶11∶1。3.4[20]。[21]。3C/N24h3。[22]。、[23]。3C/N1∶13∶150cm。C/N4Table4ThevarianceanalysistableFPC/N=1∶1262.702131.356.360.008**C/N=3∶127.07213.530.510.61C/N=5∶1239.062119.537.550.004*C/N=1∶160.95230.471.480.255C/N=3∶1122.00261.002.290.13C/N=5∶1667.552333.7821.080.000**C/N=1∶1336.502168.258.140.003**C/N=3∶11330.182665.0924.950.000**C/N=5∶11825.932912.9757.650.000**C/N=1∶1560.622280.3113.570.000**C/N=3∶1380.512190.257.140.005*C/N=5∶1218.362109.186.890.00641190296。60cm[24]。C/N5∶125cm。44。C/N1∶1C/N3∶1C/N5∶1。C/N1∶13∶150cm2∶124h80cm·d-1。C/N5∶125cm1∶124h80cm·d-1。C/N5∶152.17%C/N。C/NC/NC/N5∶1TN52.17%。[1]RomeroJA,CominFA,GarciaC.Restoredwetlandsasfilterstoremovenitrogen[J].Chemosphere,1999,39323-332.[2],,,.[J].,2006,1561385-1390.ZHANGZhen,FURong-bing,GUGuo-wei,etal.Analyseofnitrogenremovalpathwaysandtheireffectfactorsinconstructedwetland[J].E－cologyandEnvironment,2006,1561385-1390.[3]ChengHS,MohdKY,BrianS,etal.Nutrientremovalinapilotandfullscaleconstructedwetland,Putrajayacity,Malaysia[J].JournalofEnvi－ronmentalManagement,2008,88307-317.[4]SamiU,StephenPFaulkner.Useofcottongintrashtoenhancede-nitri－ficationinrestoredforestedwetlands[J].ForestEcologyandManage－ment,2006,237557-563.[5],.[J].,2006,275102-106.QINZhi-wei,HONGJian-ming.Comparisononthedifferentstuffingandflowstyleremovalofnitrogenandphosphorusinconstructedwet－lands[J].JournalofCapitalNormalUniversityNaturalScienceEdi－tion,2006,275102-106.[6],.[J].,2007,301108-110.YINJun,WENYue.Microbialcharacteristicsofconstructedwetlands[J].EnvironmentalScience&Technology,2007,301108-110.[7]Liang