不同碳源种类对好氧颗粒污泥合成PHA的影响王杰

2015,35(8)2360~2366ChinaEnvironmentalSciencePHA,*,,(,,100124),(PHA),..,PHA.,,PHA102.19mgCOD/g·VSS70.58mgCOD/g·VSS,PHA(5~26mg/gVSS).,.(PHA)X703.1A10006923(2015)08236007EffectofvarioustypescarbonsourceonthesynthesisofPHAofaerobicgranularsludge.WANGJie,PENGYong-zhen*,YANGXiong,WANGShu-ying(EngineeringResearchCenterofBeijing,KeyLaboratoryofBeijingforWaterQualityScienceandWaterEnvironmentRecoveryEngineering,BeijingUniversityofTechnology,Beijing100124,China).ChinaEnvironmentalScience,2015,35(8)2360~2366AbstractThestableperformanceofaerobicgranularsludgewouldbeaffectedbyvariousfactors.Thetypeofcarbonsourcewasreportedtohaveimpactonthesynthesisofpolyhydroxyacidlipid(PHA),consequentlyaffectthestabilityoftheaerobicgranularsludge.Inthisstudy,theaerobicgranularsludgewascultivatedwiththemixedcarbonsource,andtheeffectofdifferentcarbonsourceonthePHAsynthesizedbyaerobicgranularsludgewasinvestigated.Thetestswereconductedwith8kindsofcarbonsourceincludingsodiumacetate,methanol,glucose,maltose,starch,sugar,glutamicsodiumandpeptone.TheresultsshowedthattheaerobicgranularsludgehadbetterperformancefortransformingthesodiumacetateandsugartoPHA,withthestoragecapacityreachedupto102.19mgCOD/g·VSSand70.58mgCOD/g·VSS,respectively.Thiswassignificantlyhigherthanthevalueusingtheothercarbonsource,whichwasintherangeof5~20mg/g·VSS.Therefore,itwasbeneficifaltomaintainthestableperformanceofgranularsludgebyusingsugarandsodiumacetateascarbonsource.However,theloweststorageofPHAwasfoundwhenmethanolwasused.Keywordsaerobicgranularsludgecarbonstoragepolymerspolyhydroxyacidlipid(PHA)zoogloeastability,,,,,[1].,,,[2].[3],.[46],pH[79],[1011],[1213],(EPS),.,,(PHA),.,,20141227(2012ZX0730200206);*,,[e-mail]8PHA2361,[14].,,[15];,,PHA[16],,,[2,17].,.Wen[14]PHA;Fang[18]--(PHB);[19].,,,,.,.,,.11.110L(SBR).7L,70%,(SRT)10~12d.,6h,5min,115min,180min,30~1min(30min1min,,6h),10min,20~49min.,,0.5m3/h.,pH.A2O.,2LSBR,3,.1.,,,NO3N,1.5L,3,0.5L.4500~5000mg/L,.3.,0,15,30,60,90,120,150,180min50mL,(COD),PHA.123645871Fig.1Set-updiagramofBatchtest1.;2.pH;3.;4.;5.;6.;7.;8.pH1.2.1,COD:N:P=400:40:8.400mg/LCOD,.1mL/L,,,,,,,,,.1.2362351Table1Thecompositionoffeedwater(g/L)(g/L)NH4Cl0.15CoCl2·6H2O0.15K2HPO40.01NaMo4·2H2O0.06MgSO40.10MnCl2·2H2O0.12CaCl20.03KI0.180.05100mgCOD/L)H3BO30.150.13100mgCOD/L)FeCl3·6H2O1.500.09(100mgCOD/L)EDTA10.000.04(50mgCOD/L)CuSO4·5H2O0.030.05(50mgCOD/L)1.3PHA,PHAPHB,β(PHV)β2(PH2MV),PH2MV,PHAPHBPHV.PHA[20]10020h,PHA,Agilent6890NAgilentDB1()PHBPHV,Oehmen[21].[22].(SVI)30min,(MLSS),(MLVSS),CODcr[23].Laguna[24].(0.5mm,1.0mm,1.5mm,2.0mm2.5mm),0.5mm,0.5~1.0mm,1.0~1.5mm,1.5~2.0mm,2.0~2.5mm,2.5mm6.22.12SBR(40)Fig.2MicroscopicexaminationofSBR(40)(a),(b)(c)(d)20,40,60d8PHA2363[2(a)].2,,,.60d,,,2(d).VSS/SS0.79,SVI514mL/g,,95%88%.3,90%0.5~1.5mm.[25],,1.0~1.5mm,,0.5~1.0mm.,[1],,,.0.5~1.5mm,.,VSS/SS,0.5~1.5mm,0.79.01020304050VSS/SS(mm)(%)0.2~0.50.5~0.91.5~2.02.0~2.52.50.9~1.50.20.30.40.50.60.70.80.9VSS/SS3Fig.3Granularsludgeparticlesizedistributionandthepercentageofparticlesize2.2PHAPHA[26]:,,PHA.,,,,.PHA,.2,,PHA,102.19mgCOD/(g·VSS),,70.58mgCOD/(g·VSS),PHA,5.54mgCOD/(g·VSS),.,PHA.(VFA),CO,A(CH3COCOA)(TCA),,;VFA,,[26].[19]PHA,,PHA,,.Pijuan[27](EBRP),,PHA.,.,,,PHA.,,,CO.VFA,PHA,PHA..,,,Majone[28],236435,PHA.,PHA.,VFAPHA.,,,PHA.,,,,,,.,,,.2PHATable2ThestorageofPHAwithvarioustypescarbonsourcePHA(mgCOD/gVSS)minPHA[mgCOD/(gVSS·h)]PHA[mgCOD/(gVSS·h)]min13.21600.2295.510~59.09300.2465.580~570.581203.51785.830~510.92302.54204.0415~3020.31900.23142.560~5102.191801.48321.560~526.83301.57462.5415~305.5451.1166.510~52,PHAPHA.PHAPHA,3.51mgCOD/(gVSS·h)785.83mgCOD/(gVSS·h),PHAPHA1.48mgCOD/(gVSS·h)321.56mgCOD/(gVSS·h).,PHAPHA.,,PHA,.,PHA26.83mgCOD/(gVSS·h),,PHA1.57mgCOD/(gVSS·h),,PHA462.54mgCOD/(gVSS·h),..,PHA,,.0~5min,.,PHA.2.33CODTable3ThechangeofCODduringtheexperiment5minCOD(mg/L)180minCOD(mg/L)COD(mg/L)COD[mg/(minL)]352.4531.79320.661.83367.1724.61342.561.96383.4019.27364.131.90388.73.336.7352.000.30461.03366.1394.900.29406.0366.84339.191.94436.20254.70181.500.64442.10402.7739.330.22Majone[28]COD,COD:(1)NO3N:(2)PHA:(3):(4).:(–S)=XSTP+XGRO+(–OX)1XGRO=14.2mgCOD/mgNM(NH4+IN)(2:–SCOD;–OX;XSTP(PHA);8PHA2365XGRO;NH4+N.PHA12.14%26.35%61.51%(a)44.11%2.9%52.99%(b)62.74%22.56%14.7%(c)58.17%26.37%15.46%(d)1.35%7.76%90.89%(e)4.36%45.69%49.95%(f)6.16%93.84%(g)1.13%55.98%42.88%(h)4Fig.4Theutilizationcontionofaerobicgranularsludgeondifferentcarbonsources,NO3N,,COD.,PHA,,,COD.COD(3)(4).,COD,39.33mg/L,,,PHA.,COD94.90mg/L,PHA[4(g)].,VFA.,,PHA,.[4(f)][4(h)],40%PHA,45%,PHA.,COD320mg/L,COD.4(c)4(d),,15.46%14.70%PHA,26.37%22.56%.,,,PHA,PHA,PHA.PHA,PHA61.51%,26.35%.PHAPHA,,PHA90.89%,61.51%,PHA,7.76%236635.,PHA,PHA.33.1,PHA.,102.19mgCOD/(g·VSS);,70.58mgCOD/(g·VSS);,5.5mgCOD/(g·VSS).3.2,,PHA.[1],,,.[J].,2007,S1):4549.[2],,.[J].,2012,(2):1115.[3]WuL,PengC,PengY,etal.EffectofwastewaterCOD/Nratioonaerobicnitrifyingsludgegranulationandmicrobialpopulationshift[J].JournalofEnvironmentalSciences,2012,(24):234241.[4]SongZ,RenN,ZhangK,etal.Influenceoftemperatureonthecharacteristicsof