AA-MBBR1,1,2,2,2,2,2(1.,110168;2.,110167)[]AA-MBBR。,30%,AA-MBBRCOD、NH3-N95.36%、98.39%,5.84%、7.10%,COD16mg/L、NH3-N0.5mg/L;AA-MBBR(OTU)5527,1692,(Nitrosomonas)。AA-MBBR,。[];AA-MBBR;;[]X703.1[]A[]1005-829X(2019)01-0073-05InfluenceofdifferentfillersonthetreatmenteffectandfloradiversityofAA-MBBRsystemsLiYafeng1,LiXuguang1,ShanLianbin2,ZhaoYongjiao2,WeiChunfei2,ZhangLei2,WangYunmei2(1.SchoolofMunicipalandEnvironmentalEngineering,SchoolofShenyangJianzhuUniversity,Shenyang110168,China;2.ShenyangAcademyofEnvironmentalSciences,Shenyang110167,China)Abstract:TheinfluencesofrigidandsoftfillersontheoperationaleffectandfloradiversityofAA-MBBRsystemsinthetreatmentofdomesticsewagehavebeencontrastedandanalyzed.Theresultsshowthatwhenthedosingpro-portionsofthetwokindsoffillersareboth30%,theremovingratesofCODandNH3-NofAA-MBBRsystemfilledwithrigidfillersare95.36%,98.39%,respectively.Itis5.84%and7.10%,respectively,higherthanthatofAA-MMBRsystemfilledwithsoftfillers,andtheeffluentCODis16mg/LandNH3-Nis0.5mg/L.ThenumberofOTU(operationaltaxonomicunit)ofAA-MMBRsystemfilledwithrigidfillersis5527,whichismuchhigherthanthatofthenumber1692ofAA-MMBRsystemfilledwithsoftfillers.Furthermore,theexistenceofNitrosomonashasbeenobservedintheaerobicpoolofthesystemfillerwithrigidfillers.TheremovingeffectoftheAA-MMBRsystemfilledwithrigidfillersonpollutantsisbetterthanthatofthesystemfillerwithsoftfillers,anditsfloradiversityishigherthanthatofthesystemfillerwithsoftfillers.Keywords:fillers;AA-MBBRsystem;domesticsewage;microbialcommunityA2/O,,。AA-MBBRA2/O,A2/OMBBR,、,。,,。AA-MBBR〔1〕,,,。T-RFLP〔2〕、PCR-DGGE〔3〕、16srRNA〔4〕,,。,。,2AA-MBBR,CODNH3-N39120191IndustrialWaterTreatmentVol.39No.1Jan.,201973,AA-MBBR,2,,。11.1AA-MBBRAA-MBBR,:(A)—(A)—(MBBR)。K2、,1。1K2、,,2.2L/h。22.5L,、、4.5、8、10L,(HRT)2、3.7、4.5h。,,,。,。AA-MBBR2。2AA-MBBR1.2COD300~450mg/L,TN40mg/L,TP5mg/L,NH3-N30mg/L。、、。1.3CMAS,,,,MLSS6400mg/L,99%,1.005,SVI120mL/g。24h1、2、33,33000mg/L。1K2、2,30%,3,A2/O。3:25℃,(DO)3.0mg/L、DO1.0mg/L,pH8.0,2.2L/h,80%~100%,150%~200%。30d,CODNH3-N。CODCOD-571COD,NH3-N。1.41。11.5,,3,2。1.6、,,Mothur16SrDNARDP(Chimera),。(OTU),6,,,D16mm,10mm,2.5mm960~980kg/m313~14kg/m3()730m2/m33.8×105m2/m32019-01,39(1)742,RDPclassifier97%OTU,。MothurChao1〔5〕Simpson〔6〕。Chao1,;Simpson,Simpson,。22.1CODNH3-N,12CODNH3-N。,COD、NH3-N,3、4。3COD3,COD,、、。COD,8d,1COD89.97%、24NH3-N88.50%。,,COD。,,12。20d,1COD95.36%,COD16mg/L;2COD89.52%,COD37mg/L。,,1COD2,K2COD。4,NH3-NCOD,NH3-N30mg/L,NH3-N,NH3-N。30d,1NH3-N0.5mg/L,2NH3-N2.7mg/L,;NH3-N,198.39%,291.64%7%,。,NH3-N,K2。2.230d5。525,K21QD_HV3~V4101437,、Barcode,99557,5527OTU;OTU(97%)Chao1Simpson1QD_H10143799557552722665.77.30E-03G_CM362993531616922325.80.0311QD_H30d1CS_H30d1QD_Q30d1CS_Q30d1YN30d2G_CM30dG_ZH30dG_ZQ30dG_ZY30d3Y_CH3dY_CQ3dY_ZH30dY_ZQ30d2019-01,39(1),:AA-MBBR75G_CMV3~V4,36299,、Barcode,35316,1692OTU。5,,K21QD_HOTUG_CMOTU,1QD_HG_CM,K2。,2Chao1、SimpsonK21QD_HG_CM。2.32,。2,:K249,10,550%,(Gemmobacter)、(Rhodoferax)、(Zoogloea)、(Thermomonas),(Acinetobacter)、(Dechloromonas)、(Aquimonas)、(Sphaerotilus),K2〔7-8〕。,(Zoogloea)()(Acinetobacter),。,,〔9〕。,,。,(Sphaerotilus),10%,,,〔5〕。,。,1QD_H(Nitrosomonas),〔8〕,(NH4+)(NO2-)。,,,(Azospira),K2,〔10〕,。,K2,(Thermomonas)、(Thiothrix)、(Gemmobacter)。,30d(G_CM)(G_ZH),,15%(Thiothrix),1%。(Azospira),10%;(Parcubacteria)(Povalibacter),。,(Azospira),(Parcubacteria)。,(Azospira)〔11〕(Parcubacteria)〔12-13〕。K2,K2,K2(Nitrosomonas)〔14〕,(Gemmobacter)、(Rhodoferax)(Rhodobacter),α-(Rhodobacteraceae),,,COD〔15〕。3(1)30%,AA-MBBR2019-01,39(1)76,K2COD、NH3-N16、0.5mg/L,COD、NH3-N95.36%、98.39%。COD、NH3-N37、2.7mg/L,COD、NH3-N89.52%、91.29%,K2AA-MBBR。(2),K2AA-MBBROTU、Chao1、Simpson,AA-MBBRK2。(3)AA-MBBR3,、。K2,(Nitrosomonas)。[1]RogallaF,BavquetG,BonhoommeM.Fixedbiomasscarriersinacti-vatedsludgeplants[J].WaterPollutionResearch&ControlBrigh-ton,1988,21(12):1643-1646.[2]MichaudL,LoGiudiceA,TroussellierM,etal.Phylogeneticchar-acterizationoftheheterotrophicbacterialcommunitiesinhabitingamarinerecirculatingaquaculturesystem[J].JournalofAppliedMi-crobiology,2009,107(6):1935-1946.[3]CytrynE,MinzD,GelfandI,etal.Sulfide-oxidizingactivityandba-cterialcommunitystructureinafluidizedbedreactorfromazero-dis-chargemariculturesystem[J].EnvironmentalScienceandTechno-logy,2005,39(6):1802-1810.[4]ZhuPeng,YeYangfang,PeiFangfang,etal.Characterizingthestru-cturaldiversityofabacterialcommunityassociatedwithfiltermater-ialsinrecirculatingaquaculturesystemsofScortumbarcoo[J].Can-adianJournalofMicrobiology,2012,58(3):303-310.[5]ChaoA.Non-parametricestimationofthenumberofclassesinapo-pulation[J].ScandinavianJournalofStatistics,1984,11(4):265-270.[6]SimpsonEH.Measurementofdiversity[J].Nature,1949,163:688.[7]YeRW,ThomasSM.Microbialnitrogencycles:Physiology,geno-micsandapplications[J].CurrentOpinioninBiotechnology,2001,4(3):307-312.[8]AtkinsonBW,MudalyDD,BuxF.ContributionofPseudomonasspptophosphorusuptakeintheanoxiczoneofananaerobic-anoxic-aerobiccontinuousactivatedsludgesystem[J].WaterScienceandTechnology,2001,43(1):139-146.[9],,,.[J].,2013,26(3):5-8.[10]ReisVM,OlivaresFL,OliveiraALM,etal.TechnicalApproa-chestoinoculatemicropropa