不同微生物处理工艺对全氟化合物的去除效果

28120151ＲesearchofEnvironmentalSciencesVol．28No．1Jan．2015．J．2015281110-116．WANGKaiGUOChangshengZHANGYuanetal．ＲemovalefficiencyofperfluorinatedcompoundswithdifferentmicrobialtreatmenttechniquesJ．ＲesearchofEnvironmentalSciences2015281110-116．2014-0[e-mail]．*[e-mail]123232323123*1．2710182．1000123．1000124WAX-HPLCMSMS413PFCs〔9PFCAsC4～C124PFSAsC4、C6、C8、C10〕．9PFCAsC4～C12∑PFCAs2PFSAsC4、C8∑PFSAsPFCs．ρ∑PFCAsρ∑PFSAs81.5～135.93.7～4.6ngLρ∑PFCAsρ∑PFSAs47.4～63.32.8～3.7ngL．、PFCAsPFCAs．4PFCsPFCs．X521001-6929201501-0110-07ADOI10.13198j．issn．1001-6929．2015．01．15ＲemovalEfficiencyofPerfluorinatedCompoundswithDifferentMicrobialTreatmentTechniquesWANGKai123GUOChangsheng23ZHANGYuan23BAIYangwei23LIXiaochen1XUJian23*1．WaterConservancyandCivilEngineeringCollegeShandongAgriculturalUniversityTai'an271018China2．StateKeyLaboratoryofEnvironmentalCriteriaandＲiskAssessmentChineseＲesearchAcademyofEnvironmentalSciencesBeijing100012China3．LaboratoryofＲiverineEcologicalConservation＆TechnologyChineseＲesearchAcademyofEnvironmentalSciencesBeijing100012ChinaAbstractInordertostudytheeffectsofvarioustreatmenttechniquesontheremovalofPFCsinwastewaterandtheirtreatmentmechanismssampleswerecollectedfromfourwastewatertreatmentplantsWWTPswithdifferenttreatmenttechniquesandwereanalyzedwithhighperformanceliquidchromatography-tandemmassspectrometryHPLC-MSMSforPFCs．NineperfluoroalkylcarboxylatesPFCAsC4-C12andtwoperfluoroalkylsulfonatesPFSAsC4C8weredetectedwithvariousquantitiesinboththeinfluentsandeffluentsofWWTPs．PFCpollutionlevelsweresignificantlynegativelycorrelatedwithcarbonchainlength．ThetotalquantitiesofPFCAsandPFSAsrangedfrom81.5-135.9ngLandfrom3.7-4.6ngLininfluentsandfrom47.4-63.3ngLandfrom2.8-3.7ngLineffluentsofdifferentWWTPsrespectively．TheremovalofPFCsinwastewatershowedasimilareffectandregularitywhichwasdependentonthePFCadsorptionontheactivatedsludgewhiledifferentmicrobialtreatmenttechniquesinWWTPsexertedlittleonthePFCsremoval．KeywordswastewatertreatmentplantstreatmenttechniquesPFCsremovaladsorptionPFCsperfluorinatedcompounds、、、1-2．PFCsC—F26.3kJmol1、、、34．PFCs5-9．20095PFOSPOSF．PFCs．Murakami105ρPFOS42～635ngLρPFHpAPFHpA4.7～32.0ngLρPFOAPFOA18～25ngL．Alzaga11PFCAsPFOA＜0.34～4.32μgLPFDA＜0.5～8.2μgL．、PFCs12-13．PFCsPFOS、PFOAPFCsPFCs．43、1A、B、C、D、13PFCs〔9PFCAsC4～C124PFSAsC4、C6、C8、C10〕PFCsPFCs．11.1PFBA98%、PFPA97%、PFHxA96%、PFHpA99%、PFDoDA95%、PFBS99%、PFOS99%、PFDS99%Sigma-AldrichSt．LouisMOUSAPFUnDA96%MatrixScientificColumbiaSCUSAPFOA98%、PFNA98%、PFDA98%、PFHxS98%13C8-PFOA、13C4-PFOS50μgmLWellingtonLaboratoriesGuelphOntarioCanada．PP4℃．、．1200seriesAgilentPaloAltoCAUSA6410AAgilentPaloAltoCAUSAZorbaxEclipseXDB-C1850mm×4.6mm×1.8μmAgilentPaloAltoCAUSAVISIPＲEPTMDLSUPELCOOasisWAX6mL150mgWatersCorporationMilfordMAUSA．1.24、、1．4、PP．10000rmin10min、0.45μm500mL5ng13C8-PFOA、13C4-PFOS．WAX4mL0.1%、4mL4mL5mLminWAX4mLpH4WAXWAX6mL0.1%1mL．1Table1BasicinformationofdifferentWWTPs104tAAO4034.4%65.6%B40C20DSBＲ2570.0%30.0%111281.3AB10mmolL．70%B5min25%93min128min．30℃0.3mLmin5μL．ESIMＲM4kV、350℃8Lmin2．2Table2AnalyteionsmonitoredforLC-MSMSandconditionsoffragmentorvoltageandcollisionenergiesmzeVVPFBS299.080.012040PFSAsPFHxS413.0368.9905PFOS499.099.012055PFDS549.080.012060PFBA213.0169.0702PFPA263.0218.9703PFHxA313.0269.0803PFHpA362.9318.8805PFCAsPFOA413.0368.9905PFNA462.9418.9953PFDA513.0468.8957PFUnDA562.9518.71055PFDoDA612.8568.71051013C4-PFOS421.0376.080513C8-PFOA503.080.01406022.1．．PFCAs13C8-PFOAPFSAs13C4-PFOS．70.1、0.5、1、2、5、10、20ngmL0.99．、MDLsLOQs3.313PFCs5ngL85.7%～111.1%2.1%～11.2%USEPA100%±40%13PFCs0.1～0.3ngL0.2～1.1ngL．3、Table3ＲecoveriesMDLsandLOQsfordifferentanalyte%%ngLngLPEBA93.111.20.31.1PFPA90.39.20.20.9PFHxA94.07.80.20.8PFHpA94.07.80.20.8PFCAsPFOA102.33.50.20.8PFNA102.02.10.10.2PFDA106.32.10.10.2PFUnDA111.12.30.20.6PFDoDA109.46.30.10.5PFBS107.75.20.10.3PFSAsPFHxS96.75.20.10.3PFOS85.74.20.10.3PFDS89.93.70.10.32.2、PFCs1.14、PFHxSPFDS9PFCAs∑PFCAs2PFSAs∑PFSAs100%、PFCs．4ρPFBA51.0～96.7ngLρPFPA、ρPFHxA6.2～15.75.0～23.4ngLPFCAsPFSAs0.6～3.4ngLPFCs．Arvaniti14-15、PFCsPFOAρ25～85ngLPFOSρ4.1～40.0ngLPFBA、PFPAPFHxA．PAN167、PFCs、ρPFOAPFCsPFCsPFCsPFCsPEBA、PFPA、PFHxA、PFHpA、PFBS、PFHxSPFCsPFNA、PFDA、PFUnDA、PFDoDA、PFDS17．PFCsPFOAPFOSPFOSPFOAPFOAPFOSPFCsPFCsPFCs18-1942111PFCAsPFBA、PFPA、PFHXAPFCs．4ρ∑PFCAsρ∑PFSAs81.5～135.93.7～4.6ngL．Bossi20ρ∑PFCAsρ∑PFSAs15.0～19.73.4～11.5ngLGUO21ρ∑PFCAsρ∑PFSAs21.5～25.82.7～100.9ngLPAN16ρ∑PFCAsρ∑PFSAs0.3～37.40.1～5.5ngL．PFCAsPFSAsPFCAsPFOSPFCAsKd22-24．ρPFCsA＞D＞CρPFCsKim25-26PFCs．4PFCsPFCs．ρPFBA27.9～41.0ngLρPFPA、ρPFHxA4.1～7.13.7～8.6ngLPFCs0.4～3.2ngL．4ρ∑PFCAs、ρ∑PFSAs47.4～63.32.8～3.7ngLPFCAs．1、PFCsFig.1ClassificationsandquantitiesofdifferentPFCsintheinfluentandeffluentoffourWWTPs4、PFCAsC4～C120.01ρPFCAsPFCs、Kd．PFCsPFCs27ρPFCAs．A、B、D、PFCAsPFHxAC6PFPAC528、．2.3PFCs4．4APFPAPFCAsC4～C9PFOSPFCAsC10～C12PFSAsBPFBA、PFPA、PFHxA、PFOAPFCsCPFBA、PFPA、PFHpA、PFOAPFOSPFCsρPFPADPFCAsC4～C7PFOSPFCsPFOA．412PFCs4～6．311284Table4ＲemovalofdifferentanalyteinfourWWTPs%ABCDPFBA66.2119.5051.9466