不同温度下曝气生物滤池运行效能与微生物群落结构

106Vol．10No．620166ChineseJournalofEnvironmentalEngineeringJun．2016*266100BiostyrBAF。18℃BAFCODNH+4-N60%18～22℃BAFCODNH+4-N22℃BAFCODNH+4-N。PCＲ-DGGEBAFPCＲBAF、。BAF、BAF。BiostyrPCＲ-DGGEqPCＲX703.1A1673-9108201606-2800-07DOI10．12030/j．cjee．201501153MicrobialcommunitystructureandtheperformanceofbiologicalaeratedfilterunderdifferenttemperaturesDouNashaWangLinCollegeofEnvironmentalScienceandEngineeringOceanUniversityofChinaQingdao266100ChinaAbstractABiostyrbiologicalaeratedfilterBAFwasappliedtotreatmunicipalwastewater．TheeffectsoftemperatureonBAFnutrientremovalefficiencyandmicrobialcommunitystructurewereinvestigated．There-movalefficienciesofchemicaloxygendemandCODandammonianitrogenNH+4-Nwerebelow60%whenthetemperaturewasbelow18℃．Whenthetemperaturewasbetween18℃and22℃theremovalefficienciesofCODandNH+4-Nwerestabilizedandtheremovalrateswerehigherthanthatobservedfortemperaturesbelow18℃．Whenthetemperaturewashigherthan22℃theremovalefficiencieswerepositivelycorrelatedwithtemperatureremovalefficiencyincreasedsignificantlyasthewatertemperatureincreased．BasedonaPCＲ-DGGEanalysisthemultiformityoftotalbacteriainthemicrobialcommunitiesathighertemperatureswasbetterthanthatobservedatalowertemperature．BasedonaquantitativePCＲanalysisthetotalbacterialdensityam-moniaoxidizingbacteriaandnitrifyingbacteriaintheBAFincreasedasthetemperatureincreasedwhichwasconsistentwiththetrendsobservedforthepollutantremovalrate．Themicrobialcommunitystructureinthefilterbecamelesscomplexandthedensityofbacteriadecreasedatalowtemperature．TheseresultsindicatedthattheBAFhadoptimaleffectsatlowtemperatures．KeywordsBiostyrbiologicalaeratedfiltermicrobialcommunitystructurePCＲ-DGGEqPCＲ2008ZX07010-008-042009GGB010122015－01－222015－03－181983—。[e-mail]*[e-mail]erBAF1、、2。BiostyrBAFBAF———bio-styrene3。BAF、。64-5、6-78-9。、、、。。10。1112。。BiostyrBAFPCＲ-DGGEpolymerasechainre-action-denaturinggradientgelelectrophoresisqPCＲreal-timequantitativePCＲdetectingsystem、BAF。11.135km214m3/d131。1Fig.1MainprocessofMaidaoWWTP、、Multiflo-300、Biostyr、Multiflo-300、UV、MultifloBiostyrUV。。、。BiostyrBAF813.81m×16.70m×8.12m231m27.7m/hbiostyrene6mm3.5m7200m3。2BiostyrBAF、、、14。1.2BiostyrBAFMultiflo1。1.35、、100、200300cm。Bio-styrBAF14.5、18.623.2℃100cm200cm300cm1BiostyrBAFTable1QualityofBiostyrBAFinfluentCODmg/L130.697.3116.4NH+4-Nmg/L39.919.730.1SSmg/L922143pH7.66.77.21082102BiostyrBAFFig.2SchematicofBiostyrBAF3。《》415CODchemicaloxygendemandNH+4-NSSsuspendedsolid。pH。1.41.4.1DNADP302DNA－20℃。1.4.2PCＲ-DGGEPCＲGC338F5'-CGCCCGGGGCGCGCCCCGGGGCGGGGCGGGGGCGCGGGGGGCCTACGGGAGGCAGCAG-3'518Ｒ5'-ATTACCGCGGCTGCTGG-3'。PCＲ50μL10×buffer5μLTaqDNA0.4μL2.5mmoldNTP3.2μL2μLDNA2μLddH2O35.4μL。PCＲ94℃5min94℃30s55℃30s72℃30s3072℃10min4℃。PCＲ5μL10×bufferBio-rad8%30%～60%150V60℃4h。DGGEDGGE。1.4.3qPCＲqPCＲBacteria、AOBammol-liaoxidizingbacteria、NOBnitrite-oxi-dizingbacteriaNitrobacterNitrospira216-19。PCＲ95℃5min95℃30s15s57℃AOB55℃nitrobacter50℃nitrospira65℃72℃20s40。25μL2×TransStartTMqPCＲSuperMix12μL5μM1μLDNA2μLddH2O9μL。2PCＲTable2PrimersusedinPCＲstudiesBacterial16SrDNA121f5'-AGGAAGGTGGGGATGAC-3'238r5'-CGGCTTTCTGGGATTGG-3'CTO189fA/B5'-GGAGＲAAAGCAGGGGATCG-3'AOBCTO189fC5'-GGAGGAAAGTAGGGGATCG-3'ＲT1r5'-CGTCCTCTCAGACCAＲCTACTG-3'NitrobacterFGPS872-f5'-CTAAAACTCAAAGGAATTGA-3'FGPS1269-r5'-TTTTTTGAGATTTGCTAG-3'NitrospiraNSＲ1113f5'-CCTGCTTTCAGTTGCTACCG-3'NSＲ1264r5'-GTTTGCAGCGCTTTGTACCG-3'2pH7.11～7.375∶1CODNH+4-N。3h。2.1CODNH+4-N208263。18℃COD57.9%COD51.5mg/L22℃CODCOD50mg/LGB18918-2002A64.9%。18℃20。3CODNH+4-NFig.3CODandNH+4-Nremovalefficiencyatdifferenttemperatures14～18℃NH+4-N60%NH+4-N14.5mg/L19～21.5℃NH+4-NNH+4-N11.9mg/L21.5～27.5℃NH+4-N65.3%21.5℃79.8%26℃。BAF2120～35℃。18℃BAFCODNH+4-N18～22℃BAFCODNH+4-N22℃BAFCODNH+4-N。NH+4-NCOD。2.2DP302TiangenDNAGC-338F518ＲPCＲ。DGGE414.5、18.623.2℃BAF23.2、18.614.5℃1095。DGGE5①1478937894②23610215℃③51。BAFCODNH+4-N。3DGGEUPGAMA6。3623.2℃18.6℃86.6%23.2℃14.5℃56.7%。15℃22。BAFBAF。3082104DGGEFig.4DGGEprofilesofbiofilm5DGGEFig.5DGGEbandsidentificationchart3Table3Similarmatrixofdifferentbelts℃%23.218.6℃14.5℃23.210086.656.718.686.610063.814.556.763.81006DGGEFig.6ClusteringtreeofDGGEprofiles2.3214.5、18.623.2℃BAF、AOB、NitrobacterNitrospi-raＲeal-timePCＲ4。4、AOB、NitrobacterNitrospiraTable4CellnumbersofbacteriaAOBNitrobacterandNitrospirainBAFcopy/500μL14.5℃18.6℃23.2℃5.60×1071.79×1082.34×108AOB1.38×1064.05×1064.97×106Nitrobacter7.16×1056.94×1063.21×106Nitrospira2.40×1065.30×1065.06×107①18.6℃BAF、AOB、NOBNitrobacterNitrospira14.5℃3.2、2.93.9。14.5～18.6℃CODNH+4-NNH+4-NCOD58.3%NH+4-N14.5℃54.7%18.5℃68.2%60.3%②23.2℃BAF、AOB、NOBNitrobacterNitrospira18.6℃1.3、1.24.418.6～23.2℃BAF64.2%NH+4-N66.9%NH+4-N9.8mg/L③AOBNOB23.2℃4.97×106copy/500μL5.38×107copy/500μL18℃NH+4-N④18℃BAFCODNH+4-N。3BiostyrBAF40826BAFCODNH+4-N。18℃COD57.9%22℃CODCOD50mg/LGB18918-2002A64.9%18℃NH+4-N60%21.5～26℃NH+4-N65.3%79.8%。BAFBAF18℃BAF、。BAF。NH+4-NCOD。115℃。1．．2013772409-2413LuHongyuMaWenchengZhangLiangetal．Ad-vancedtreatmentofmixingdyeingwastewaterusingbio-logicalaeratedfilterprocess．ChineseJournalofEnviron-mentalEngineering2013772409-2413inChi-nese2FengYanYuYanzh