不同污泥龄厌氧氨氧化菌的脱氮效能及其动力学特性张杰

50820188JOUＲNALOFHAＲBININSTITUTEOFTECHNOLOGYVol.50No.8Aug．2018DOI10．11918/j．issn．0367-6234．20170713712111121．1001242．150090SＲTSBＲNO2－－NNrNO2－－NNs．21d12dNr0．590kg/m3·d0．493kg/m3·dMLVSSNs0．178kg/kg·d0．297kg/kg·dMonodNO2－－Nvmax0．406d－10．826d－1Ks23．3mg/L95．3mg/LNO2－－N．X703．1A0367－6234201808－0001－07NitrogenremovalefficiencyandkineticcharacteristicsofanammoxbacteriaatdifferentsludgeagesZHANGJie12ZHANGZewen1LIDong1GUOYuezhou1LIShuai121．KeyLaboratoryofBeijingforWaterQualityScienceandWaterEnvironmentＲecoveryEngineeringBeijingUniversityofTechnologyBeijing100124China2．StateKeyLaboratoryofUrbanWaterＲesourceandEnvironmentHarbinInstituteofTechnologyHarbin150090ChinaAbstractTheexperimentwastoexploretheeffectofdifferentSＲTonthenitrogenremovalefficiencyandkineticcharacteristicsofAnAOBbystudyingthevariationofnitriteremovalloadsNrnitritesludgeloadsNsandkineticcharacteristicswithgradientdecreaseofSＲTinaSBＲ．TheresultsshowedthatwithSＲTdecreasingfrom21dto12dNrdecreasedfrom0．590kg/m3·dto0．493kg/m3·dwhileNsincreasedfrom0．178kg/kg·dto0．297kg/kg·dandtheN-removalperformanceofthewholesystemwasreducedbuttheN-removalefficiencyofunitmassofAnAOBwassignificantlyimproved．TheMonodmodelcanwellsimulatethekineticbehaviorofAnAOBatdifferentSＲT．Themaximumspecificdegradationratevmaxincreasedfrom0．406d－1to0．826d－1andthesemi-saturatedconstantKsincreasedfrom23．3mg/Lto95．3mg/LwhichindicatedthatgradientlydecreasingSＲTcanscreenandpurifyAnAOBwithfastergrowratewhiletheaffinityforthesubstrategraduallydeterioratedandthestabilitydecreased．KeywordsSＲTAnammoxNrNskineticcharacteristicsvmaxKs2017－07－112014000028833TD021938—1976—[e-mail]tionanammox20901－23－4．—、、5－67NH+4+1．32NO－2+0．066HCO－3+0．13H→+1．02N2+0．256NO－3+0．066CH2O0．5N0．15+2．03H2O．8－9．、、10．11－1213－14．SBＲNO2－－NNO2－－N．1516－17．11．1SBＲ1100mm700mm10．6L．2/38h．25±2℃．1Fig．1Experimentalequipmentofthereactor1．2200mg/L、200mg/LSBＲMLSS5320mg/L1∶1．22∶0．2580%78．5%1．05kg/m3·d10．0L．NH4+－NNO2－－N200mg/LNaHCO35∶1KH2PO42．5mg/LCaCl212．5mg/L1．00mL/LEDTA15g/LCoCl2·6H2O0．25g/LZnSO4·7H2O0．42g/LCuSO4·5H2O0．24g/LMnCl2·4H2O0．98g/LH3BO40．015g/LNiCl2·2H2O0．020g/LNaWO4·2H2O0．060g/L．7NH4+－NNO2－－N1∶1．32NH4+－NNO2－－N1∶1NO2－－NNO2－－N．1．3200mg/LNH4+－NNO2－－N21、18、1512dNO2－－NNO2－－N．．1．4N－1－pH、DOWTWpH/Oxi340iWTWpH296Oxi296MLSSMLVSS．22．1NO2－－N、NO2－－N、NO2－－NMLVSS2、3．121d．14．5dTd=ln2·SＲT1814．5d．28·2·50MLVSS3952mg/L3420mg/L9MLVSS1∶1．29∶0．2520NO2－－N20．6mg/L90．8%NO2－－N0．590kg/m3·dMLVSSNO2－－N0．178kg/kg·d．2NO2－－NFig．2VariationofNO2－－Nconcentrationandbiomassintheprocessofreducingsludgeage3NO2－－NFig．3VariationofNO2－－Nloadsintheprocessofreducingsludgeage2118d．12．5dTd=ln2·SＲTMLVSS30MLVSS2917mg/L31MLVSS1∶1．31∶0．24．2、340NO2－－N32．2mg/L85．3%NO2－－N0．560kg/m3·d5．5%0．030kg/m3·d1940NO2－－N0．193kg/kg·d0．015kg/kg·d．315d．MLVSS512310mg/L1∶1．34∶0．271865NO2－－N0．232kg/kg·d400．193kg/kg·d65NO2－－N38．8mg/L81．2%NO2－－N0．502kg/m3·d404．1%0．058kg/m3·d．6512d1∶1．28∶0．26．90MLVSS1657mg/LNO2－－N49．7mg/L76．8%NO2－－N0．493kg/m3·dNO2－－N0．297kg/kg·dNO2－－N．NO2－－NNO2－－NNO2－－N．2．2Monod．．SBＲ20、、、“”NO2－－NMonod211NO2－－NXtS0－Se=Ksv()max1S()e+1vmax．1Xmg/LtS0NO2－－Nmg/LSetNO2－－Nmg/L·3·8Ksv=12vmaxNO2－－NvmaxNO2－－Nd－1．121d8h．1．1Tab．1Dataofcycletestt/minρ/mg·L－1t/minρ/mg·L－10209．00210．030182．330183．460156．160156．490135．890136．4120112．6120113．218080．918080．324054．824053．830032．630033．636028．336028．648016．248016．3MonodNO2－－N4．Ｒ20．9890．9810．985MonodNO2－－N．4NO2－－Nvmax0．4030．408d－1NO2－－N0．406d－1Ks23．123．6mg/L23．3mg/L．vmaxKsy=57．31x+2．46．a=57．31b=2．461XtS0－Se=57．31Se+2．46．221NO2－－NSe22．5%．2～415～7．Ｒ20．9702～4MonodNO2－－N．41Fig．4KineticsofANAMMOXundercycletestinstage12Tab．2Comparisonofmeasuredandpredictedvaluest/minρ/mg·L－1/%0208．0208．0030181．3182．30．560155．4157．51．390135．3133．71．2120111．9111．9018078．275．23．824052．149．84．430033．434．42．936027．126．23．348016．116．41．83．5%2～4NO2－－N．vmaxKs．4～7·4·5021、18、1512dvmax0．406、0．411、0．5160．826d－1．vmax．201812d、．52Fig．5KineticsofANAMMOXundercycletestinstage263Fig．6KineticsofANAMMOXundercycletestinstage374Fig．7KineticsofANAMMOXundercycletestinstage4Ks．21dEPS·5·822Ks23．3mg/LTang23Ks=23mg/L．Ks．12dKs95．3mg/L23．3mg/L．MonodNO2－－NvmaxKs12d．32－4Tab．3Comparisonofmeasuredandpredictedvaluesinstage2－4t/min234ρ/mg·L－1/%ρ/mg·L－1/%ρ/mg·L－1/%0208．0208．00205．0205．00207．0207．0030183．9187．51．9184．5185．60．6188．0188．40．260165．0168．32．0165．6166．60．6168．9170．50．990145．8150．02．9147．8149．91．4153．2154．40．8120128．9132．72．9132．2134．51．7137．9139．71．3180103．2101．12．0108．9106．42．3116．5113．12．924081．277．44．789．485．14．897．593．54．130062．059．44．269．467．42．981．478．43．736048．147．12．154．754．80．265．267．23．148030．931．82．937．338．94．349．149．61．03121d12d0．590kg/m3·d0．493kg/m3·d0．178kg/kg·d0．297kg/kg·d．2MonodNO2－－N．MonodvmaxKs．3．1．J．2009354509－515．TIANZhiyongLIDongYANGHongetal．Start-upandnitrogenremovalperformanceofanaerobicammoniumoxidationbio-filterJ．JournalofBeijingUniversityofTechnology2009354509－515．2．J．201430121804－1816．DOI10．13345/j．cjb．140241．ZHANGZhengzheJIYuxinCHENHuietal．ApplicationandobstaclesofANAMMOXprocessJ．ChineseJournalofBiotechnology201430121804－1816．DOI10．13345/j．cjb．140241