不同盐度反硝化过程N2O积累及还原特性巩有奎

37220192EnvironmentalEngineeringVol．37No．2Feb．2019N2O*12131.2640002.1001243.1000823LSBＲNO－3COD/N=5.0NO－2N2ON2OMonod。。NaCl020g/LNO－3、NO－2N2O17.6714.15139.33mg/g·h6.577.4133.17mg/g·h。N2O。N2OＲN2ONaＲNO－3NiＲNO－2N2O。N2ONO－3NO－2N2O。N2ODOI10.13205/j．hjgc．201902011N2OACCUMULATIONANDＲEDUCTIONCHAＲACTEＲISTICSDUＲINGDENITＲIFICATIONPＲOCESSOFDIFFEＲENTSALINITIESGONGYou-kui12ＲENLi-fang1LIYong-bo31.DepartmentofArchitectureEngineeringYantaiVocationalCollegeYantai264000China2.KeyLaboratoryofBeijingforWaterQualityScienceandWaterEnvironmentＲecoveryEngineeringBeijingUniversityofTechnologyBeijing100124China3.BeijingGeneralMunicipalEngineeringDesign＆ＲesearchInstituteCo．LtdBeijing100082ChinaAbstractUsingNO－3aselectronacceptorandethanolascarbonsourcetheeffectofdifferentsalinitiesontheNO－2andN2Oaccumulationandreductionduringdenitrificationprocesswasinvestigatedina3LlabscalesequencingbatchreactorSBＲwiththeinitialC/Nratioregulatedat5.0andtheN2OreductionprocesswasconductedMonodequationfitting．Theresultsshowedthatthereductionratedecreasedwiththeincreasingofsalinity．WhentheNaClsalinityincreasedfrom0to20g/LreductionrateofNO－3NO－2andN2Odecreasedfrom17.6714.15139.33mg/g·hto6.577.4133.17mg/g·hrespectively．Thehalf－saturationconstantoftheN2OreductionincreasedwithincreaseofthesalinityandtheN2OreductasehadahigheraffinitytoitssubstrateN2Ounderlowersalinityshowingahighreductionrate．TheaccumulationofN2OattheinitialofthedenitrificationprocesswasduetothatthesynthesisrateofN2OＲN2OreductasewaslowerthanthatofNaＲNO－3reductaseandNiＲNO－2reductase．TheinhibitionofhighsalinityonN2OreductionwasmuchgreaterthanthatonNO－3andNO－2reductionactivitieswhichwasthemainreasonforN2Oaccumulationindenitrificationprocessunderhighsalinity．KeywordssalinitydenitrificationN2Oaccumulation*201800221177005。2018－09－250、C、NP1-2。C/N。N2O2N2OCO2200～300。Ｒavishankara3N2O21。N2ON2O-NTN1%～25%4。DO、C/NpHN2O5。N2O6-7。NO－3－NN2。NaＲNiＲNoＲN2OＲNO－2N2O8-10。。N2O。NO－2N2O。11.1SBＲρNH4+、ρNO－2ρNO－3＜1mg/L。。pH=7.5～7.8ρDO＜0.05mg/L25±1℃。12h、。1.2SBＲ3L。WTW340iDO、pHDO、pH。1.31.3.1NO－3ρMLSS2500～2800mg/LNO－3C/N5.0。NaCl051020g/L。150min。1.3.2N2ON2O5minN2O051020g/LρN2O20mg/L30min。1.3.1。1.430min。Multi340iWTWDOpH。COD、NH+4－N、NO－2－N、NO－3－NMLVSS11UNISENSEN2OPicoammeterPA20000.01μmol/LN2O12。1.5NO－3N2O。N2OMonodrN2O=kmSKs+S1rN2ON2Omg/g·hkmN2Omg/g·hSN2Omg/LKsN2Omg/L。22.1NO－31NO－3、NO－2N2O。NaCl05g/LNO－3NO－2。45minρNO－3－N2mg/LNO－2－N5.96.1mg/L45minNO－3NO－2。＜5g/LNO－3－NNO－2－N。NaCl20g/L120minNO－2－N3.9mg/L。NaClNO－3NO－2－N3637。NO－3－NNO－3－NNO－2－NNaClNO－3－N10NO－2－NNO－2－N。。。—□—ρNaCl=0—△—ρNaCl=5g/L——ρNaCl=10g/L—☆—ρNaCl=20g/L。1NO－3NO－3NO－2N2OFig．1EffectofNaClconcentrationsonNO－3NO－2andN2OinthedenitrificationofNO－3NO－3N2O。N2OＲNaＲNiＲN2OＲN2ON2N2O13。N2ONO－3NO－2N2O14。0N2O0.268mg/L15minN2N2O。N2O14。5g/LNO－3－NNO－2－NN2O0.442mg/L64.9%60minN2O。15-16。20g/LN2O1.146mg/L327.6%。NaClN2ON2O。2.2N2O2N2O。ρN2O18.5mg/LNaCl0N2O3.6min。ρNaCl=1020g/L。NO－3NO－2N2O。N2ON2ON22。—□—ρNaCl=0g/L—△—ρNaCl=5g/L——ρNaCl=10g/L—☆—ρNaCl=20g/L。2N2OFig．2EffectofsalinityonN2OreductionprocessN2O。Pan141N2O。NaＲNO－3NO－2N2O17。NO－2N2O。462N2ON2ONO－21b、cρNO－26.1mg/LHNO2N2O。N2ONO－3NO－2NO－3NO－2N2O1。2.31NO－3、NO－2N2O。ρNaCl=5g/LNO－3－NNO－2－NNO－3－NNO－2－N20g/LNO－3NaＲＲNO－2NiＲＲNaＲＲ17.67mg/g·h6.57mg/g·h62.82%NiＲＲ14.15mg/g·h7.41mg/g·h47.63%。NaＲＲNiＲＲNiＲＲNaＲＲNO－3－NNO－2NO－3NO－21b。1NO－3、NO－2N2OTable1ＲeductionrateofNO－3NO－2andN2OunderdifferentsalinityρNaCl/g·L－1NaＲＲ/mg·g－1·h－1ICNaＲＲ/%NiＲＲ/mg·g－1·h－1ICNiＲＲ/%N2OＲＲ/mg·g－1·h－1ICN2OＲＲ/%017.6710014.15100139.33100517.590.4514.5－2.4735107.6722.721012.2330.7810.7823.2459.8157.07206.5762.827.4147.6333.1776.19N2ON2OＲＲNaＲＲNiＲＲ1N2O。NaCl20g/LN2O1.146mg/LNO－2。NaＲＲNiＲＲN2OＲＲNaCl。、DO、FNA。5g/LN2OＲＲN2O22.72%。NaCl20g/L76.19%NO－3NO－2。ZhaoN2O10NaCl20g/LN2O90%76.19%。。。、15。。ρNaCl20g/LN2O。2.4N2OMonod16。Monod1OriginN2O2。NaCl0.49Pan18pH=7.0N2O0.4mg/L0。KsNaCl20g/LKs1.55mgN/L。KsKs。KsN2ON2ONO－3。N2O。。2N2OTable2Salinityeffectonhalf-saturationconstantKsofN2Oreductionprocess/g·L－1051020/mg·L－10.490.711.111.55563731020g/L。5g/LNaClNO－3NO－2N2O22.72%。NaCl20g/LNO－3、NO－2N2O17.6714.15139.33mg/g·h6.577.4133.17mg/g·h62.82%、47.63%76.19%。N2O。2N2OＲNaＲNiＲN2ON2OＲN2O。1GlennEThompsonTLFryeＲetal．EffectsofsalinityongrowthandevapotranspirationofTyphadomingensisPersJ．AquatBot199552175-912BassinJPKleerebezemＲMuyzerGetal．EffectofdifferentsaltadaptationstrategiesonthemicrobialdiversityactivityandsettlingofnitrifyingsludgeinsequencingbatchreactorsJ．ApplMicrobiolBiotechnol20129331281-1294．3ＲavishankaraAＲDanielJSPortmannＲW．NitrousoxideN2Othedominantozone-depletingsubstanceemittedinthe21stcenturyJ．Science20093265949123-125．4YeLNiBJLawYYetal．Anovelmethodologytoquantifynitrousoxideemissionsfromfull－scalewastewatertreatmentsystemswithsurfaceaeratorsJ．WaterＲesearch2014481257-268．5MassaraTMMalamisSGuisasolAetal．AreviewonnitrousoxideN2OemissionsduringbiologicalnutrientremovalfrommunicipalwastewaterandsludgerejectwaterJ．ScienceoftheTotalEnvironment2017596/59715106-123．6ZhaoWWangYYLinXetal．IdentificationofthesalinityeffectonN2Oproductionpathwayduringnitrificationusingstepwiseinhibitionand15NisotopelabelingmethodsJ．ChemicalEngineeringJo