不同营养元素组合对高浓度制药废水产沼气的影响研究胡勇

:2009-03-20:　(1981-),,,:,E-mail:[e-mail]:,E-mail:[e-mail]胡　勇,孔垂雪,尹小波(　,　610041)　:N,P,SFe,Co,Ni,。,COD,,2,N,P,FeSO4·7H2O,NiCl2·6H2O,CoCl2·6H2O100mg·L-1,200mg·L-1,300mg·L-1,1.0mg·L-1,1.0mg·L-1,18%,COD1.29%。:;;:X703;S216.4　　:A　　:1000-1166(2009)03-0019-04EffectsofDifferentCombinationofNutritionElementsonBiogasProductionwithHigh-concentrationPharmaceu-ticalWastewater/HUYong,KONGChui-xue,YINXiao-bo/(BiogasInstituteofMinistryofAgriculture,KeyLaboratoryofEnergyMicrobiologyandApplication,Chengdu610041,China)Abstract:AdditionofmainnutrientelementsN,P,SandtraceelementsFe,Co,Nicouldimproveefficiencyofbiogasproductionfromauxotrophicpharmaceuticalwastewater.BiogasproductionandCODdegradationweredeterminedwithdif-ferentcombinationofnutritionelementsandconcentration.Resultsshowedthatthebiogasproductionincreasedby18%,andCODdegradationrateincreasedby1.29%underadditivecombinationofN,P,FeSO4·7H2O,NiCl2·6H2O,CoCl2·6H2Oatconcentrationof100mg·L-1,200mg·L-1,300mg·L-1,1.0mg·L-1,1.0mg·L-1respectively.Keywords:biogasfermentation;additives;nutritionelement;pharmaceuticalwastewater;methanogens1　　　,,(、、),,、。,[1]。,,,[2]。、、、、、。,,,。。2　　　,、。,,[2]。,,RonowheGunnarsson[3],C5H7O5NP0.01S0.1,、、、,。,N,P(Fe,Co,Ni,Mo,Se,W)[4]。Tanaka[5],,,。SighSingh[6],19ChinaBiogas2009,27(3)Cu(NO3)2,480.036m3·kg-122.22%。,,,,。,[2]。3　3.1　试验材料3.1.1　,,,,。1:1　COD/mg·L-1pHTS/g·L-1TSS/g·L-1807694.256.3334.74　　,,pH7.1,,TS5.5%,VS46.6%。3.1.2　:NH4Cl,NaH2PO4·2H2O,FeSO4·7H2O,NiCl2·6H2O,CoCl2·6H2ON,P,S,Fe,Ni,Co。　　:500,(,,),(GC2010,GC7AG)。:500,1。,35℃±1℃,。1　3.1.3　。GC-7AG,Po-rapakQS,80～100,TCD,50℃,TCD70℃,50mA,(99.99%)。3.2　试验设计6(6),,,,。2。2　/mg·L-1NPNiCl2·6H2OCoCl2·6H2OFeSO4·7H2O1()0000021002001.01.030031001000.21.01000415008000.210.010051002010.00.200650010010.01.0100　　N,P,FeSO4·7H2O,;NiCl2·2H2OCoCl2·2H2O,,,。,N,P,FeSO4·7H2O,NiCl2·2H2OCoCl2·2H2O。NP,。3.3　试验方法6500,。300,100,,(35℃±1℃)。,1,,。2113。2　4　　　,。15,20ChinaBiogas2009,27(3)COD,3。4.1.1　CODCOD,。2COD,18%,COD1.29%(3,4)。3　　　COD　mg·L-1　　mL　COD　mg·L-1　COD　%　　mL　CH4　%　　mL18076968.5189897.65313549.7155828076968.585698.94369053.2196338076968.5139798.27326052.1169848076968.5130098.39334554.7183058076968.5182597.74319052.7168168076968.539798.29324057.918764.1.2　,,,226%。4.2　讨论4.2.1　N,PCOD,,,COD∶N∶P350∶5∶1[2]。N100mg·L-1,P200mg·L-1COD80769mg·L-1,COD∶N∶P350∶5∶1230mg·L-1N46mg·L-1P,100mg·L-1N200mg·L-1P,,P。,。,,[7]。4.2.2　S,。,,,,,,。,,,。,2,,32,11.7%;2,2。。:,,COD,,。,COD/SO2-415,SO2-4[8]。SO2-4FeSO4·7H2O,1000mg·L-1,COD/SO2-480,。4.2.3　Fe,Co,Ni,,、、,、、、、、、[2]。[9]21ChinaBiogas2009,27(3),Fe,Co,Ni。,0.3～0.8mmol·L-1;2FeSO4·7H2O300mg·L-1,1.08mmol·L-1,3FeSO4·7H2O1000mg·L-1,3.59mmol·L-1,23,FeSO4·7H2O2,,FeSO4·7H2O300mg·L-1。,;4Co2,2,。(CoCl2·2H2O)1.0mg·L-1。Ni,NiF420,M[10,11]。56Ni2,,(NiCl2·2H2O)1.0mg·L-1。,,,。,。,[2]。,。4.2.4　,,COD,18%,26%。,2,。5　　　,。,,2,18%,26%,COD1.29%。,,;,[7]。。:[1]　,.[M].:.2000.[2]　,[M].:.2005.[3]　RonnowPH,GunnarssonLAH.Responseofgrowthandmethaneproductiontolimitingamountsofsulfideandammoniaintwothermophilicmethanogenicbacteria[J].FEMSMicrobiolLett,1982,14(4):311-315.[4]　SpeeceRE.Anaerobicbiotechnologyforindustrialwastewater[M].Nashville,TNUSA:ArchaePress,1996.222-226.[5]　TanakaS,LeeYH.Controlofsulfatereductionbymo-lybdateinanaerobicdigestion[J].WaterScienceandTechnology.1997,36(12):143-150.[6]　SinghS,SighSK.Effectofcupricnitrateonaccelationofbiogasproduction[J].EnergyConversionandManaga-ment.1996,37(4):417-419.[7]　,,.[M]..2003[8]　,,.COD/SO42-[J]..1992,14(1)[9]　,.[J]..2001,23(3):116-118.[10]WhitmanWB,WolfeRS.PresenceofnickelinfactorF430fromMethanobacteriumbryantii[J].Biochem.Bio-phys.Res.Commun.1980,92(4):1196-1201.[11]DiekertG,KonheiserU,PiechullaL,etal.Nickelre-quirementandfactorF430contentofmethanogenicbacte-ria[J].JBact.1981,148(2):459-464.22ChinaBiogas2009,27(3)