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47220152JOUＲNALOFHAＲBININSTITUTEOFTECHNOLOGYVol.47No.2Feb．2015doi10．11918/j．issn．0367-6234．2015．02．014殷娣娣1，陆纳新2，袁君2，高乃云1，戎文磊2，周圣东21．2000922．214031．220±3L·mol－1·s－12655±15L·mol－1·s－1．3＞＞．IOM．TU991A0367－6234201502－0074－05InactivationofMicrocystisaeruginosabydifferentpre-oxidantsYINDidi1LUNaxin2YUANJun2GAONaiyun1ＲONGWenlei2ZHOUShengdong21．PollutionControlandＲesourceＲeuseStateKeyLaboratoryTongjiUniversity200092ShanghaiChina2．WuxiWaterWorksCo．Ltd214031WuxiJiangsuChinaAbstractComparisonofthetreatmenteffectandmechanismsofdifferentpreoxidantsonalgalcellswereanalyzedbytheexperimentsofinactivationofM．aeruginosawithpotassiumpermanganateozoneandsodiumhypochlorite．TheresultsshowedthatthedecreaseofquantumyieldofM．aeruginosacausedbychlorinationandozonationbothfollowedsecond-orderkinetics．Sodiumhypochloritecausedthecelllysiswiththequantumyieldrateconstantof220±3L·mol－1·s－1andresultedinthereleaseofintracellularorganicmatterIOMregardlessofinitialconcentration．OzonehadaprofoundoxidationeffectonM．aeruginosacellsandwouldrapidlycausethecelllysiswiththerateconstantof2655±15L·mol－1·s－1regardlessoftheinitialozonedosages．ThesequenceofthequantumyieldrateconstantsofthreeoxidationswasO3＞NaClO＞KMnO4．HoweverbothozoneandsodiumhypochloritecausedthecelllysisandledtothereleaseofIOMmoreeasilythanpotassiumpermanganatewhichwassuggestedbytheresultsofscanningelectronmicroscopy．Keywordspre-oxidationMicrocystisaeruginosasecond-orderkineticsalgalactivityrelease2014－07－10．511783212012ZX07403－0012008ZX07421－002201200721100502009－K7－4．1977—1950—．gaonaiyun@sina．com．MCs、AOM1－2．．．EOM．MC－LＲAdda、．、、IOM．、MCs3－5．6－7MC－LＲ．．3．11.1MicrocystisaeruginosaFACHB－912BG11．25±1℃、2400lx、12h12h．6.4×106·mL－1．1．21．．．．．!#$!%&’()*+,-#11.320±2℃pH7.5±0.1．250mL．1．4PHYTO－PAMWalzUV－765/0.7μmTMIIHACHDPD8Philips．SigmaMilli－QMillipore．22.12.1.16.4×106·mL－156.4～225.7μmol·L－12～8mg/L600s．2．!#$%&%&&’&&$&&(&&#&&)&&!*+,!#-*,./012%-’./*1(./*1)./*13./*1$./*1#./*1./*122100s．EOM．100s．100sEOM．600s600s．2.1.2Y00.43．·57·256.4μmol·L－12mg/LY056.4μmol·L－1．56.4～225.7μmol·L－12～8mg/L60sY0．－dYdt=kYYClt．1ctctkY=－lnYct/Y0ct．2．56.4μmol·L－184.6～225.7μmol·L－1．31．!#$%&’()*+,,,-#!%&’()*+,,,#.%&’()*+,,$/#-%&’()*+,,/0#1%&’()*+,--1#0%&’()*+,.+.#1+,#.+,#1+-#.+-#1+2#.+2#1.#..1.#.,..#.,1.#.-..#.-1.#.2.(34!!.5#$64&’()*+,)753ct1ctcCl/μmol·L－1kY/L·mol－1·s－1Ｒ284.6221.000.871112.8223.330.901141.0223.400.936169.2223.400.976197.5221.650.967225.7218.380.9651．84.6～225.7μmol·L－1kY220±3L·mol－1·s－1kY．Ｒ2．6－7kY．kYIOM．8－10．2．22．2．16.4×106·mL－122.4～60.6μmol·L－11.073～2.909mg/L500s．4．!#!$%#%$&#&$$#$&$$%$$!$$’$$#$$!()&$*!+,(-&&.’+,(-&’%/+,(-&/$*+,(-&.#!+,(-%0$0+,(-1!#$2(1+,3-4&24440s．EOM．40s．1.0731.1841.426mg/L100180300s．2.2．2Y00.45．22.424.7μmol·L－11.0731.184mg/L1800s0.30.2524.7μmol·L－1．29.7～60.6μmol·L－11.426～2.909mg/L．1.426mg/L330s2.909mg/L90s．、·67·47IOM．、EOM．．22.424.7μmol·L－129.7μmol·L－133.5～60.6μmol·L－1．52．!!#$%#!%#$&#!&#$’#!’#$(#!!#!!%!!#!!%$!#!!&!!#!!&$!#!!’!!)*+,-./%.01-2*#$#!1’’#$3+,-./%’4#53+,-./%5!#53+,-./%5ct2ctcO3/μmol·L－1kY/L·mol－1·s－1Ｒ233.52641.80.94038.62666.80.90060.62663.70.9922．33.5～60.6μmol·L－1kY2655±15L·mol－1·s－1kY．12137．IOM．2.3IOM．、kY5.00～19.386220±32655±15L·mol－1·s－1＞＞．、IOM6．a3mg/L1minb8mg/L5minc20mg/L2hd60mg/L4he6SEM·77·26、6dIOM6e．6－7IOM．IOM、．、、AOM．．31218．38～223．40L·mol－1·s－1．84.6112.8μmol·L－1．．22641．8～2666．8L·mol－1·s－1．．3＞＞．．1．、J．200824620－24．2LILeiGAONaiyunDENGYangetal．Characterizationofintracellular＆extracellularalgaeorganicmattersAOMofMicrocysticaeruginosaandformationofAOM-associateddisinfectionbyproductsandodor＆tastecompoundsJ．WaterＲesearch20124641233－1240．3DALYＲIHOLBＲOOKESJD．EffectofchlorinationonMicrocystisaeruginosacellintegrityandsubsequentmicrocystinreleaseanddegradationJ．EnvironmentalScience＆Technology200741124447－4453．4ＲODＲIGUEZEMAJADOMEMEＲILUOTOJetal．OxidationofmicrocystinsbypermanganatereactionkineticsandimplicationsforwatertreatmentJ．WaterＲesearch2007411102－110．5．－LＲJ．2009377919－924．6．J．2014425721－729．7LILeiSHAOChenLINTsairfuhetal．KineticsofcellinactivationtoxinreleaseanddegradationduringpermanganationofMicrocystisaeruginosaJ．EnvironmentalScience＆Technology20144852885－2892．8BADEＲHHOIGNEJ．DeterminationofozoneinwaterbytheindigomethodJ．WaterＲesearch1981154449－456．9LEEWWESTEＲHOFFPCＲOUEJP．DissolvedorganicnitrogenasaprecursorforchloroformdichloroacetonitrileN－NitrosodimethylamineandtrichloronitromethaneJ．EnvironmentalScienceTechnology200741155485－5490．10FANGJingyunYANGXinMAJunetal．CharacterizationofalgalorganicmatterandformationofDBPsfromchloraminationJ．WaterＲesearch201044205897－5906．(刘彤)·87·47