不同原水及处理工艺的末梢水中有机物致突变性研究

2012Y2－000201988－。[e-mail]··1112211．5100802．510440、3。3、。3。。。Ｒ123．6A1004－8685201403－0341－04StudyonmutagenicorganiccompoundsindifferentsourcewaterandperipheralwaterwithdifferentdisinfectionprocessHUANGQin－yi*XINGXiu－meiZHAOZi－hanZHONGNiWANGDe－dongSONGHong*SchoolofPublicHealthSunYat－senUniversityGuangzhou510080ChinaAbstractObjectiveTounderstandthemutagenicityoforganicextractsfromsourcewaterandperipheralwaterinwaterworksofwesteastandsouthpartsofsomesoutherncitysoastodiscusstheeffectofdifferentsourcewateranddisinfectionprocessesonthemutagenicityoforganicsinperipheralwater．MethodsTheCometassaywasusedtodetectthemutagenicityoforganicextractsfromthesourcewaterandperipheralwaterofthreewaterworks．ＲesultsOrganicextractsfromsourcewaterandpe-ripheralwaterofthreewaterworksshoweddifferentdegreesofmutagenicityandthemutagenicityofperipheralwaterwassignifi-cantlyhigherthanthatofsourcewater．ConclusionMutagenicenhancementfactorsarefoundinbothsourcewaterandperiph-eralwaterofthreewaterworks．Watertreatmenttechnologycanincreasethemutagenicityofwaterbutthebiologicalsignificanceofmutagenicityoftheorganicextractsremainedtobefurtherconfirmed．Theresultssuggesteditwasnecessarytoconcerntherelationshipbetweensourcewaterwatertreatmentunitandthemutagenicityfactorsofwater．KeyWordsPeripheralwaterOrganiccompoundsMutagenicity、3、《》GB3838－20021ⅡⅠ2Ⅲ3Ⅱ－Ⅲ4。3“－”、5。3、。11．1201210－201211、36200L。1．2XAD－25．5cm15cm。310min45℃～50℃。1．31．3．17～8。106/DMEM5ml。3450ml、150ml、50ml、12．5ml·143·20142243ChinJHealthLabTecFeb2014Vol24No3350ml、25ml、12．5ml0．2mmol/L3。37℃2hPBS。Trevigen30minＲIPA3h。25min25V、300mA30min。Tris21。PI25min2。1．3．2515nm～560nm100Cometscore、。1．4SPSS13．0。χ2P＜0．05。210%9．31μm±5．49μm0．45±0．5099．93μm±10．63μm41．82±12．51。2．1350ml50ml～450ml、、P＜0．05、、r0．39、0．57、0．440．93、0．96、0．940．91、0．90、0．91P＜0．05。50mlP＜0．051。2．2350ml、12．5ml～50ml、25ml～50mlP＜0．05、、r0．28、0．94、0．87P＜0．0512．5ml～50ml、、P＜0．05、、r0．30、0．90、0．82P＜0．0525ml～50ml、12．5ml～50mlP＜0．05、、r0．47、0．95、0．88P＜0．05。12．5ml、25ml、50mlP＜0．052。1、、ml%μm50．010ac7．3±4．5ac0．2±0．3ac150．030*ac31．7±7．1*ac4．9±2．6*ac450．054*ac61．0±8．4*ac18．6±5．7*ac50．033*b41．0±4．5*ab12．4±4．5*ab150．055*ab60．7±6．6*ab22．4±5．7*ab450．0100*ab102．8±10．0*ab56．3±13．6*ab50．028*b29．6±4．0*bc4．0±1．7*bc150．041*bc52．1±6．8*bc17．9±5．0*bc450．082*bc82．6±8．7*bc37．7±8．1*bc*abcP＜0．05。2、、ml%μm12．511c8．05±5．54*ac0．33±0．46ac25．013ac16．84±5．27*ac1．47±1．22*ac50．039*ac50．01±7．30*ac17．34±6．27*ac12．535*ab22．78±5．13*ab2．72±1．61*ab25．042*b50．67±7．08*ab18．26±6．07*ab50．072*b69．05±8．04*ab29．04±9．15*ab12．512c17．62±3．91*bc1．69±0．99*bc25．042*b46．07±6．52*bc16．37±5．69*bc50．068*b71．20±5．89*bc31．27±8．66*bc*abcP＜0．05。2．3312．5ml12．5mlP＜0．053。·243·20142243ChinJHealthLabTecFeb2014Vol24No33、、12．5ml%μm50．0ml%μm98．7±4．80．4±0．5a10a7．3±4．5a0．2±0．3a118．05±5．540．33±0．46a39a50．01±7．30a17．34±6．27a10*9．6±5．4a0．6±0．6a33a41．0±4．5a12．4±4．5a35*22．78±5．13a2．72±1．61a72a69．05±8．04a29．04±9．15a107．5±4．5a0．3±0．4a28a29．6±4．0a4．0±1．7a1217．62±3．91a1．69±0．99a68a71．20±5．89a31．27±8．66aaP＜0．05。3、、3DNADNA－。3DNADNA50ml、12．5ml673。、3DNA。DNADNADNA、DNA。50ml、25mlP＜0．0512．5ml25ml12．5ml。3。。。89。1011。DNA＞＞DNA＞＞3DNADNA。12DNA。DNA313。DNA。“－”、14“－”DNA。。《》GB15193．1－200315。。1．GB3838－2002S．2002．2．J．201127181－4．348·343·20142243ChinJHealthLabTecFeb2014Vol24No3SH、。、。ICPGSHMＲP1mＲNAMＲP2、MＲP5mＲNAGSHMＲP2、MＲP5MＲP1mＲNA。MＲP－GSH。MＲP－GSH。1St－PierreMVSerranoMAMaciasＲIetal．Expressionofmem-bersofthemultidrugresistanceproteinfamilyinhumantermplacentaJ．AmJPhysiolＲegulIntegrCompPhysiol20002794Ｒ1495－Ｒ1503．2BallatoriNTruongAT．GlutathioneasaprimaryosmoticdrivingforceinhepaticbileformationJ．AmJPhysiol19922635617－624．3．PXFLPSIL－10J．2005365517－520．4ChaouatGMelianiAAMartalJetal．IL－10preventsnaturallyoccurringfetallossintheCBA×DBA/2matingcombinationandlo-caldefectinIL－10productioninthisabortion2pronecombinationiscorrectedbyinvivoinjectionofIFN－γJ．JImmunol199515494261－4268．5PathakBSheibaniLLeeＲH．CholestasisofpregnancyJ．Ob-stetGynecolClinNorthAm2010372269－282．6PanCPerumalswamiPV．Pregnancy－relatedliverdiseasesJ．ClinLiverDis2011151199－208．7JoshiDJamesAQuagliaAetal．LiverdiseaseinpregnancyJ．Lancet20103759714594－605．8Kozowska－ＲupDanutaCzekajPiotr．BarrierroleofABCfamilyofproteinsinhumanplacentaJ．GinekolPol201182156－63．9DeeleyＲGWestlakeCColeSP．Transmembranetransportofendo－andxenobioticsbymammalianATP－bindingcassettemultidrugresistanceproteinsJ．PhysiolＲev2006863849－899．10PatelPWeerasekeraNHitchinsMetal．Semiquantitativeex-pressionanalysisofMDＲ3FIC1BSEPOATP－AOATP－COATP－DOATP－EandNTCPgenetranscriptsin1stand3rdtri-mesterhumanplacentaJ．Placenta200324139－44．2013－10－163433．J．20118437－38．4．J．200632523－26．5．J．2006552－53．6．J．··2004166352－354358．7．J．2005224289－291．8．D．2000．9．J．200316462－63．10GuzzellaLDiCaterinoFMonarcaSetal．Detectionofmuta-gensinwater－distributionsystemsafterdisinfectionJ．MutatＲes2006608172－81．11ClaxtonLDPegramＲSchenckKMetal．Integrateddisinfectionby－productsresearchsalmonellamutagenicityofwaterconcen-tratesdisinfectedbychlorinationandozonation/postchlorinationJ．JToxicolEnvironHealthA200871171187－1194．12．A．．．2008C．．20081－7．13．D．2008．14．J．200118316