不同植物细胞壁的体外发酵特征及其对甲烷产生的贡献张元庆

,2006,37(10),992~998ActaVeterinariaetZootechnicaSinica,,*(,100094):(IC)6,6,,72h(GP)(B)(C)(lag)(P010001,010001,010073010002)48h,CO2(P010001),,(22121%),(8194%)6(P=015316)(P=015063),(P=010179)(TVFA)(P=010002),(P=014680)(P01055),:;;;:S81615:A:0366-6964(2006)10-0992-07:2005-12-30:(30125033):(1979-),,,,,Tel:[phone];Fax:[phone];E-mail:[e-mail]*:,Tel:[phone];Fax:[phone];E-mail:[e-mail]rizationofDifferentSourcePlantCellWallsandTheirContributiontotheMethaneEmissioninRuminantAnimalsinvitroZHANGYuan-qing,WEIJ-ian,MENGQing-xiang*(CollegeofAnimalScienceandTechnology,ChinaAgriculturalUniversity,Beijing100094,China)Abstract:Thetypesandthequantitiesofmonosaccharideconsistedinthesixdifferentplantcellwallsweredeterminedbytheionchromatographinthisexperiment.Andtheruminalfermentablecharacterizationandtherelativecontributiontothemethaneyieldinruminantanimalswerecom-paredwiththesixdifferentplantcellwallsbyincubatingthemwiththeruminalfluidasthemethodofgasproduction(MenkeandSteingass,1988).Thegasproductionin72h,themax-imumgasproduction,therateandthelagtimeofgasproductiondifferedgreatlyamongdifferentplantcellwalls(Pvaluewere010001,010001,010073and010002,respectively).Thepercentageofmethaneandthecarbondioxideinthegascompostiondifferedsignificantly(P010001).TheactivitiesofCMCaseandAvicelaseinthefermentedfluidhadnoremarkabledifference(P=015316and015063,respectively)butthexylanaseactivitiesweredifferentgreatly(P=010179).Thetotalvolatilefattyacid,themolarpercentageofeachkindofvolatile10:fattyacidexceptbutyrate(P=014680)weredifferentsignificantly(P01055).Itwasconclu-dedthatthetypesandthequantitiesofmonosaccharideconsistinthesixdifferentplantcellwallsandtheirruminalfermentablecharacterizationandtherelativecontributiontothemethaneyieldinruminantdifferedgreatly.Keywords:plantcellwall;monosaccharide;rumenfermentation;methane,[1],,,[2],H2,H2,,,6,,1111(CR)(WH)(CS)(SBP)(AL)(SH)1mm,215g1%(SDS)100mL1h4,[3](FD-4,)72h,48h11250mg50mL,1mL500mg/mL(Ino,Novachemo,MBG,)72%H2SO4(w/w,AR),25e1h,28mL(Milliporesystem),125e1h,(WhatmanGF/D,47mm),50mL,4e[4,5](DINAX2500;:CarboPacPA20;:PulsedElectrochemicalDetection),:NaOH5mmol/L()2mmol/L10mmol/L();0138mL/min;25LL10mg/kg:(Fuc,SigmaF-2252)(Ala,Fluka10840)(Gal,Amresco0637)(Glu,SigmaG-8270)(Man,AR)(Rha,Novachemo)(Xyl,AR),(500mg/mL)11133(@,340kg),2(08:0016:00),(6916%,24%,212%,118%,112%,1%-112%)3kg,3kg,4kg14d114MenkeSteingass[6]30mL39e200mg(DM)(100mL,)39e1153,72h,72h,,,(8000g,15min),1mL,25%012mL,,,10000g15min,(SP-3420;:FID;:PEG-20M,2000mm013mm)993371,(NaOH:2mmol/L),(NaOH:5mmol/L)Fig.1Thestandardgraphofionchromatographyforthemonosaccharidedetermination.Theleftoneisthestandardgraphforthemonosaccharidefromdicotyledonousplantcellwalls(NaOHflowis2mmol/L)andtherightoneisforthemonosaccharidefrommonocotyledonousplantcellwalls(NaOHflowis5mmol/L)VFA,:145e,200e,FID220e,()45mL/min,35mL/min,250mL/min,8,11,016LL[7];()015mL015mL1%(SigmaC-4888FlukaPh101SigmaX-0627)PBS(0105mol/L;pH710)39e30min,[8,9]DM(SP-3420;:TCD;:TDX-01,:120e,190e,150e,150e,,30mL/min,,011mL)48hCO2CH4H22116SAS(1996)[10]NON-LINEAR,GP=B*(1-exp(-c*(t-lag))),GPt012000g(DM)(mL);B012000g(mL);c(/h);t(h);lag(h)SAS(GLM)22111,3,(X/C)(01795)(01087),[1],[11,12]21272h72h3,,399410:2,,Fig.2Thestandardgraphofgaschromatographyforthedeterminationofvolatilefattyacidandgascomposition.TheleftoneisfortheVFAdeterminationandtherightoneisforthegascompositiondetermination1Table1Themonosaccharidecompositionsofdifferentplantcellwallsas%DMMonosaccharidecompositionSourceofplantcellwallsCRWSCSSBPALSHArabinose3158216211973112391158150Galacose113501391103418111152100Glucose281012910531120421405710651173Xylose14140231111215031692814015198Mannose010001000100113401002148Total631173156213831595188016X/C015140179501401010870149801307X/C(Xyl)(Glu)CRWSCSSBPALSHX/Cstandsfortheratioofxylosecontenttotheglucosecontent.TheCR,WS,CS,SBP,ALandSHareshortfortheCh-ineseryegrass,wheatstraw,cornstalk,sugarbeatpulp,alfalfaandsoyhulls,respectively.,,72h;,72h3,2,72h(P010001)(P010001)(P=010073)(P=010002)72h3,Varel[1]2H2CH4CO2,6,(P=013074),99537CR(r)WS(t)CS(o)SBP(@)AL(*)SH(p)200mg()CR(r),WS(t),CS(o),SBP(@),AL(*)andSH(p)areshortforChineseryegrass,wheatstraw,cornstalk,sugarbeatpulp,alfalfaandsoyhulls,respec-tively.Datapointsrepresentthemeanofthreedetermina-tionsadjustedtothe200mgdrymatterofsubstrates372hFig.3Gasproductionfromdifferentmillplantcellwallsbyruminalfluidfor72hinvitro(P010001),,Moss[2],(1)72h4,(SH),(SBP),(CR)72h(3)72h(2),272h48hTable2Thedynamicfermentationparameterswithin72hfermentedinvitroandthegascompositionofdifferentplantcellwallsin48has%DMCRWSCSSBPALSHSEMP=GP/mL413e4814c3511d6410b4610c8114a0186010001B/mL411e6117c4118d7316b5518c8916a3110010001C/h01055a01028c01034bc01038abc01027c01052ab01004010073Lag/h01397c21560a01769b11305b11052b11712b01194010002H2/%01054010260103101056010430103601010013074CH4/%8194c19106a22121a15196b19154a21153a01744010001CO2/%91100a80191c77176c83199b80142c78144c01740010001GP=B*(1-exp(-c*(t-lag))),B012000gDM(mL);c(/