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ASBＲ350108ASBＲ、ASBＲ。ASBＲ。VFA93mg/L54mg/LCa2+EPS3。80dCOD150d0．5～4．0mm15．0～38．5m/h。MLSS7．8g/LCOD97%～99%。TU992A1000－4602201819－0030－07510080802015J01199ＲapidGranulationofSludgeinAnaerobicSequencingBatchＲeactorStrategyMechanismandPopulationStructureSHAOXiang-wenZHANGTing-tingAICui-lingLIUDa-xinCHENQi-youCollegeofCivilEngineeringFuzhouUniversityFuzhou350108ChinaAbstractAnaerobicsequencingbatchreactorASBＲhasreceivedmuchattentionduetoitslowinvestmentsimpleoperationandflexiblecontrol．Howevertheproblemssuchasthedifficultytoformgranuleslimititsapplication．ThereforerapidcultureofanaerobicgranularsludgewasinvestigatedinASBＲfedwithglucose．ToenhancethegrowthofgranularsludgeASBＲwasoperatedwithlongfeedingtimeandCa2+wasdosed．TheresultsshowedthatprolongingthefeedingtimeanddosingCa2+couldac-celeratetheformationofanaerobicgranulesinASBＲ．MeanwhileextensionoffeedingtimecouldreducetheaccumulationofVFAanddecreasetheconcentrationofaceticacid．Thepeakconcentrationofaceticaciddecreasedfrom93mg/Lto54mg/LinacyclethusboostedthegrowthofMethanosaetawhichwasinfavorofthegranulationofsludge．AddingsomeamountofCa2+couldpromotethebacteriacellstose-cretemorePNandPSinEPSinwhichthecontentsofPNandPSwerenearlytripledandobviouslyplayedanimportantroleforthestructureandmaintenanceofgranules．After80daysofoperationgran-uleswithfilamentousmicroorganismswerethedominantformofmicrobialaggregatesinthereactor．Fur-therthefeedingtimewasdecreasedfrom120minto30minandinfluentCODgraduallyincreasedwithgradientresultingintheformationofsphericalbacteriamaturedinthegranularsludgein150dayswith·03·3419201810CHINAWATEＲ＆WASTEWATEＲVol．34No．19Oct．2018adiameterfrom0．5－4．0mmandsettlingrateof15．0－38．5m/h．TheMLSSwas7．8g/LinreactorandtheremovalefficiencyofCODreached97%－99%．KeywordsASBＲgranularsludgefeedingtimeaceticacidEPSASBＲ1SSVSS2～4。2090Dague300d1mm5VFA6。UASB7、EGSB8150d、。910、、、。ASBＲ。EPS。1112～14。EPS、、、。ASBＲEPSASBＲASBＲ。11.1ASBＲ4．5L150mm375mm33±1℃5min30s50r/min。1.2COD∶N∶P=300∶5∶1。1500mg/LCaCO3。NaCl120mg/LMgCl2·6H2O49mg/LNa2SO443mg/LFeSO4·7H2O5．53mg/LMnCl2·4H2O2．02mg/LZnCl20．67mg/LNiCl2·6H2O0．65mg/LCaCl2·H2O0．59mg/LCoCl2·6H2O0．48mg/LCuCl2·6H2O0．16mg/LH3BO30．063mg/LNa2MoO4·2H2O0．0045mg/L。1.3。MLSS、MLVSS26．14、19．77g/L2L。1.4、。38h1/3HＲT24h。30min、410min、30min、10min。CaCl2COD。30d。31～80d120min、330min、20min、10min100mg/LCaCl2。81～180d30min、400min、40min、10min100mg/LCaCl2。1.5CODpHVFA－3．0、2．5、2．0、1．5、1．0、0．5、0．2mm·13·．watergasheat．comASBＲ3419、、、1200mg/L2～4gVSS/L33±1℃。22.1ASBＲ180dCODVFA。12COD、CODVFA。COD1000mg/L21000mg/L30dCOD3000mg/LCOD85%VFA265mg/LpH6．8SS100mg/LASBＲ。进水ＣＯＤ出水ＣＯＤ去除率80007000600050004000300020001000COD/（mg·Ｌ-1）０180t/d160140120100806040201009080706050403020100去除率/%1CODFig．1VariationofCODandCODremovalwithtime40035030025020015010050出水VFA/（mg·Ｌ-1）０180t/d160140120100806040202VFAFig．2VariationofVFAineffluentwithtimeSS750mg/LCOD2500mg/L。COD500mg/LCOD90%VFA100mg/L。2.22.2.13VFA。７００６００５００４００３００２００１００VFA/（mg·Ｌ-1）０１2345678t/h启动阶段形成阶段成熟阶段a.试验组ＶＦＡ７００６００５００４００３００２００１００VFA/（mg·Ｌ-1）０１2345678t/h启动阶段形成阶段成熟阶段b.对照组ＶＦＡ１６０１４０１２０１００８０６０４０２０乙酸/（mg·Ｌ-1）０１2345678t/h启动阶段形成阶段成熟阶段c.试验组乙酸１６０１４０１２０１００８０６０４０２０乙酸/（mg·Ｌ-1）０１2345678t/h启动阶段形成阶段成熟阶段d.对照组乙酸3VFAFig．3VariationofVFAandaceticacidinacycleatdifferentstagesVFA·23·3419．watergasheat．com。VFA30min120minVFA445mg/L218mg/L93mg/L54mg/L。4。30min20min。a.形成阶段颗粒污泥外表面b.形成阶段颗粒污泥内部c.成熟阶段颗粒污泥外表面d.成熟阶段颗粒污泥内部4Fig．4SEMphotographsofsludgeatdifferentstages80d30minVFA0．5h645mg/L140mg/L。。20min40min。150dVFA。。180d＞0．5mm80%COD6500mg/LCOD95%。。MethanosaetaMethano-sarcina。MethanosarcinaMethanosaeta1516。MethanosaetaMethanosarcinaMonodS*70mg/L70mg/LMethanosaetaMethanosarcina。VFAMethanosaeta。120min30min2．3hMethano-sarcinaMethanosarcina。MethanosarcinaMethanosaeta17。2.2.2EPSCa2+EPSEPS。EPS5。PNPS100mg/LCa2+EPSPS6．13mg/gMLSS24．14mg/gMLSSCa2+PN11．02mg/gMLSS39．83mg/gMLSS。0．5～1．5mm1．5mm15%6。PNPS1．5mm。PNPS。EPS·33·．watergasheat．comASBＲ341918。6050403020100EPS含量/（mg·g-1MLSS）启动形成成熟启动形成成熟蛋白质多糖试验组对照组5EPSFig．5VariationofEPSduringgranulationprocess65d95d125d155d180d50403020100分布比例/%粒径/mm0.2~0.50.5~11~1.51.5~22~2.52.5~46Fig．6SizedistributionofgranulesduringprocessofgranulationEPSPNPN。19EPS。Ca2+EPSZeta20。2.32.3.1。665d50%0．2～0．5mm155d0．5～1．5mm52%1．5～2．5mm23%4．0mm。、。21。2.3.27。＜1．5mm＞1．5mm38．5m/h。。Schmidt22。45403530252015/（m·h-1）0.5/mm01.01.52.02.57Fig．7Variationofsettlingrateofgranularsludge2.3.38。1.61.41.21.00.80.60.40.20/（gCOD·g-1VSS·d-1）3040128150t/d8Fig．8VariationofspecificmethanogenicactivitiesSMAduringexperiment·43·3419．watergasheat．com40d、、0．122、0．641、0．3710．142gCOD/gVSS·d。150d0．723、0．973、0．8240．635gCOD/gVSS·d。2.4、EPS。、。Methanosaeta、、。。Ca2+EPSEPSEPS。。Metha-nosaetaMethanosarcina。EPSPN。EPS。3ASBＲ180d30d、COD。1TauseefSMAbbasiTAbbasiSA．Energyrecoveryfromwastewaterswithhigh-rateanaerobicdigestersJ．Ｒenewable＆SustainableEnergyＲeviews2013191704－741．2MasséDIMasseLVervilleAetal．Thestart-upofanaerobicsequencingbatchreactorsat20℃and25℃forthetreatmentofslaughterhousewastewaterJ．Jour-nalofChemicalTechnology＆Biotechnology2010764393－400．3IntanooPSuttikulTLeethochawalitMetal．Hydrogenproductionfromalcoholwastewaterwit