采用含硫铁化学污泥作为反硝化电子供体进行焦化废水中总氮深度去除付炳炳

39720187ENVIＲONMENTALSCIENCEVol．39No．7Jul．201811112*131．5100062．5100063．510006C/N、、、、．、．NO－3-N、NO－2-N74.54±0.57mg·L－1、1.11±0.19mg·L－118h2.78±1.08mg·L－1、2.87±0.71mg·L－1TONNO－3-N+NO－2-N90.0%NO－3-NNO－2-N12.06mmol·L·d－1、7.74mmol·L·d－1．．X703.1A0250-3301201807-3262-09DOI10．13227/j．hjkx．2017112202017-11-232017-12-2721377040517782832015B0202350052015A0202150081991～[e-mail]*[e-mail]trogenＲemovalfromCokingWastewaterUsingSulfideIron-containingSludgeasaDenitrificationElectronDonorFUBing-bing1PANJian-xin1MAJing-de1WANGFeng1WUHai-zhen2*WEIChao-hai131．SchoolofEnvironmentandEnergySouthChinaUniversityofTechnologyGuangzhou510006China2．SchoolofBiologyandBiologicalEngineeringSouthChinaUniversityofTechnologyGuangzhou510006China3．KeyLaboratoryofPollutionControlandEcologicalＲestorationinIndustryClustersMinistryofEducationGuangzhou510006ChinaAbstractIngeneralitisdifficulttoreachthetotalnitrogendischargestandardintheeffluentaftermunicipalandindustrialwastewatertreatment．TheproblemshinderingadvanceddenitrificationincludeanunstableC/Nratiointheinfluentwastewaterincreasedhydraulicloadingwithincreasingrefluxratioreducedreactionkineticshighenergyconsumptionandsecondarypollutionandhighsludgeyieldresultingfromadditionoforganiccarbonsources．Thereforedeepdenitrificationwiththeadvantagesofenergysavingsandeasyoperationisurgentlyneeded．Toaddresstheseissueschemicalironsulfidesludgecollectedafterthepretreatmentofsulfur-containingindustrialwastewaterwasusedasasolid-phaseelectrondonortoperformadvanceddenitrificationusingautotrophicdenitrifiers．Inthisstudythesecondarybiologicaleffluentofcokingwastewaterwastheinfluentfordenitrificationandtheperformanceofdenitrificationtransformationofsulfideandironinthesludgeandmicrobialcommunitychangeswereinvestigated．Theoptimalreactionconditionsandeffectrangeofthetechnologyfordeepdenitrificationofwastewaterwerethencalculated．WhentheconcentrationsofNO－3-NandNO－2-Nintheinfluentwere74.54±0.57and1.11±0.19mg·L－1respectivelythecorrespondingconcentrationsintheeffluentwerereducedto2.78±1.08and2.87±0.71mg·L－1respectivelywithahydraulicretentiontimeHＲTof18h．TheremovalrateofTONNO－3-N+NO－2-Nwasashighas90.0%ofwhichthereductionrateofNO－3-NandtheaccumulationrateofNO－2-Nwere12.06and7.74mmol·L·d－1respectively．Thisstudyshowedthattheuseofchemicalsulfideironsludgeasanelectrondonorfordeepdenitrificationisofpracticalimportanceasitcouldsimplifythesubsequenttreatmentofsulfur-andiron-richchemicalsludgefinallyreachingthegoalofresourceutilization．Keywordssludgecontainingsulfurandironresourceutilizationcokingwastewaterautotrophicdenitrificationadvancednitrogenremoval7、、“”1．、、．NH+4-N5.0mg·L－1ATN15.0mg·L－123NO－3-N4．、．C/N、、567、COD89．S、S2－、Fe、Fe2+、H210～13、14～17．、．1418、．CEPA7192021．221Fe2++S→2－FeS↓1FeS2322425、pH、26．．5FeS+9NO－3+8H2→O5FeOH3+4.5N2+5SO2－4+H+2NO－3-N、NO－2-N、NO－3-N、NO－2-N．11.1、A/O/H/O、27COD1.3×103～2.8×103mg·L－1143～535mg·L－1COD95.0%99.9%28．4℃1．1Table1MaincharacteristicsofbiologicallytreatedcokingwastewatersfromthesecondstageofShaogangWWTPCOD/mg·L－1TN/mg·L－1NO－3-N/mg·L－1NO－2-N/mg·L－1NH+4-N/mg·L－1UV254/cmCN－/mg·L－1S2－/mg·L－1B/CpH140～15075～8570～80＜2＜12.4～2.6＜0.05＜0.2＜0.17.0～8.0TOCS2－1355.7mg·L－1523.7mg·L－11284.2mg·L－17.2mg·L－1．30min1LCOD5.0mg·L－1．1L10mL0.37g1L37g·L－1．EDS150μmFe51.55%362339S18.93%C15.40%O11.77%Na1.67%Ca0.56%Al0.13%FeS70.48%．1MEDSFig．1SEMofsludgeandEDSpointanalysisofsludgemarkedM1.2UASB2．5cm50cm1000mL10cm45cm．、、、．．2Fig．2Integratedverticalup-flowbiologicalsuspended-bedreactor6UASBUASBNa2S2O3·5H2O5.00g·L－1、NaNO32.00g·L－10.74g·L·d－1Thiobacillus25.73%．0.30LMLSS=3.69g·L－10.40L37.0g·L－150mL·min－120minDO＜0.10mg·L－1．HＲT、、pH、．90d25℃2d．351000mL．1.30.45μm．pHpHSX731、EVOLUTION300ThermoScientificCODCODDＲ3900HACHTOCTOCTOC-VcpnShimadzuNO－3、NO－2、SO2－4ICS-900DionexTONNO－3-NNO－2-NUV254EVOLUTION300ThermoScientificXGENESISXMEDAX．1.4EzupDNADNA．200mgBufferSCLDNABufferSPBufferSB．DNA－20℃．DNAPCＲ1%DNA．PCＲ16SrＲNAV4-V516SrＲNA515F/907Ｒ．515F462375'-GTGCCAGCMGCCGCGGTAA-3'907Ｒ5'-CCGTCAATTCMTTTＲAGTTT-3'．2xPremixTaqTakaraDalianCo．Ltd25μL、Primer-F10mmol、Primer-Ｒ10mmol、DNA60ng50μLPCＲ．PCＲ94℃4min94℃30s52℃30s72℃30s72℃10min4℃．DNA．22.12.1.13Fig．3DenitrificationperformanceofthereactorduringthestableoperationperiodHＲT．390dTON、NO－3-N、NO－2-N．TON75.37±1.42mg·L－1、NO－3-N98.96%HＲTHＲT24h95%TONNO－3-N、NO－2-N2.27±1.09mg·L－1、1.55±1.25mg·L－1．HＲT18hNO－3-N、NO－2-N8.55±1.68mg·L－1、25.45±3.65mg·L－1NO－2-N34.05%．HＲT．TON4.20mg·L·h－129Li18FeS2NO－3-N．．2.1.2NO－3-N、NO－2-N．43NO－3-N、NO－2-N、SO2－4．NO－3-N、NO－2-N75.31mg·L－1、1.58mg·L－16hNO－3-N4.29mg·L－194.15%NO－2-N48.02mg·L－161.67%6～18hNO－3-NNO－2-N48.02mg·L－10.78mg·L－112hNO－2-N22.44%HＲT=12hNO－2-N18hTON1.52mg·L－198.02%HＲT=18h．4Fig．4DenitrificationperformanceoftheintermittentlyoperatedreactorpH23dNO－3-Ndt=－KNO－3-Nn3562339NO－3452FeS+9NO－3+5H2→O2FeOH3+9NO－2+2SO2－4+4H+43FeS+9NO－2+6H2→O3FeOH3+4.5N2+3SO2－4+3OH－5NO－2-N6dNO－2-Ndt=－dNO－3-Ndt－dNO－2-Ndt=KNO－3-Nn－KNO－2-Nm=K－K'=K″6m、nKNO－3-Nmmol·L·d－1K'NO－2-Nmmol·L·d－1K″NO－2-Nmmol·L·d－1．NO－3-NNO－2-N3031．Ｒ22．12.06mmol·L·d－17.74mmol·L·d－1NO－3-N．2Table2Kineti