测定活性污泥脱氢酶活性的研究安立超

93Vol.9No.PollutionControlTeehnology16999SeP.1996JJAStudyontheMaesuremnetofDehdyrogasneeAeitvityinACitvatedSludge(,210094),INT,pH、、、,INT。INTAtractThetetarzoliumsalt,piodonitrotetrazoliumviolet(INT)wasusedtorapidlydeterminethedehdyrogenaseaetivityinaetivadsludge.TheafetorsinflueneingtheexperimentsuehaspH,teepratuer,buffersolution,reactiontime,extraetionand50onwerediseussed.TheoPtimumeodnitionsfordeterimningINTdehydrogenaseaetivitywereobtained.KeyrdsdehdyrogenaseaetivitydehydrogenaseaetivatedsludgeINT。。。。,(TTC)2[]“M。、、(2),。(INT),,,,.11.1。1.2,30。。r/min。,,339/L。l·3NIT:0.209NIT50ml,0.4%INT。;(Tir:)2I[;()。L4,TirsHcl,,(19960308)186ZmlINTlml,37℃.,3,。5ml,。,,1cm.,722485nm(A).,(A)。2.21INTINT,,37℃、pH8.515min。,485nm(A),1。0.()4060.(l8()INT(%),ù|4,00-nUCU0)1INT,(A)INT,INT,A。INT。.04%.,,INT。.04。.22,,(2)pH,(l):pH8.5.1pHpH6.07.08.08.59.010.0A0.8330.7980.8260.9740.8730.679.24pHS.5,。2,ApH,TirsHcl,。2ATitsHclA0.558().7350.178pH7.58.57.8.25INT,。。(3):,,,,A,.3A(95)A1.3011.2070.252.2`。(3、4),A。(A/min)。,15min。。ILǎV)Jtà.ùUnUCǎè。;(C)2,35~45,37。.23pH℃。℃10】2()23〔)3,〕t(nr,n)3187~--J~-~~~~`~-51025`303540t(m`n)01008600004ēuōà4.2,、、、pH,。,。9,,,(5):65432100floǎVà46(d)5,,,,。2.8。、,15imn,,,,。31,,.,:,19852,,.,:,199。3NielsenR.H.MeasuertentofthelnhibitionofRespiartionincAitvatedSludgeby·MifedeDtemrinationoftheTTCehydorgeanseAe-tivity,WaetrReeareh,1975,9(12):1179~1185.4lKaPwijkA.etalAModiifdePoreeduerfortheTTCehdyoranseTestincAtivatdelSduWaterReseacrh,1974,8(2):121~125··21—,,21。,。.,19948,,,200,5l.,,68。,,。,40%,.“”19964s188。·,。,,、。,,,、。,。,。,,,。:.96.5.18《Newcsientist》