:2001207209:(59976024);(5992004):025420096(2002)06207782041,1,1,1,2,2,1(11,100037;21,510070):PVA,,,,1417kgCOD/m3d,COD9413%,1614h,65%73%,COD1716kgCOD/m3d,812kgCOD/m3d,1317h7L/dL15.2,COD87.0%,,,:;;:TK6:A0,[14],[58],,(Methanosarcina)maxKs,PVA,,,,111111111,:,21280109/mL,10%:012%,012%,0104%K2HPO4,011%NH4Cl,0101%MgCl2,1%,pH712(HCl)100mL1111211113:11114:,,,:,,,112UASB11211:250mL,21280109/mL:012%,012%,0104%K2HPO4,011%NH4Cl,0101%MgCl2,015%,015%,pH712()236200212ACTAENERGIAESOLARISSINICAVol123,No16Dec.,2002©1995-2005TsinghuaTongfangOpticalDiscCo.,Ltd.Allrightsreserved.CODcr800011000mg/L:,CODcr600011000mg/L,pH315610pH610NaOH55d,:UASB,(A),(B),7cm,50cm,2L,810,11212CODcr()COD(SP2304,)2211211112%,,,5d,,,,,,,,,,21112,4:;;CaCl2:1,211Table1OrthogonaldesignwithdifferentfactorsandlevelsA/cm-2B/%C/hD136224220412h33620142h2Table2orthogonalexperimentaldesignA1B2C3D411111A1B1C1D121222A1B2C2D231333A1B3C3D342123A2B1C2D352231A2B2C3D162312A2B3C1D273132A3B1C3D283213A3B2C1D393321A3B3C2D1A()B(Alginate)C()D()1Fig11Ralationshipoftheindexesandthecharacteristics2Fig12Thesumofthegasproductionbycross2test14,92,,1,,A3,B2,C2,D3;,A3,B2,C2,D2,D2,D3,9776:©1995-2005TsinghuaTongfangOpticalDiscCo.,Ltd.Allrightsreserved.,D32,No15No19,A3,B2,C2,D3,2036;4%;1h;2h212212113Fig13Gasproduction2LUASB,55d,3,,8500mL/d4350mL/d,214COD4,,,COD,COD9413%5416%65%73%,,4CODFig14CODremovingrate&CODloadingratebymanmadewastewater2121255d,,34dAB1317h1419hB7dCOD,8d5Fig15Gasproductionbysoybeancakewatewater5,,14000mL/d,710L/(Ld),5250mL/d,2163L/(Ld)217,1512A65%,B6CODFig16CODremovingrate&CODloadingratebysoybeancakewastewaterCOD6,,COD812kgCOD/m3d513kgCOD/m3d,,COD1716kgCOD/m3dCOD8710%7816%COD7516%62119%,COD,COD08723©1995-2005TsinghuaTongfangOpticalDiscCo.,Ltd.Allrightsreserved.31),2),,:3620;4%;1h;2h3)2LUASB,55d,COD4)34d,,COD,COD,[][1]LinYingfeng,ChenKuochen.Denitrificationandmethanogenesisinaco2immobilizedmixedculturesys2tem[J].WatRes,1995,29(1):3543.[2]KarubeI,etal.Methaneproductionfromwastewatersbyimmobilizedmethanogenicbacteriz[J].BiotechnolandBioeng,1980,12:847857.[3].[J].1994,15(5):1014.[4],.[J].,1998,28(2):3539.[5].2[J].,1997,17(3):268270.[6].[J].,1998,18(4):356359.[7].[J].,1999,5():8083.[8].(Methanosarcina)[J].,1999,20(2):6369.ANEWMETHODFORMETHANOGENIMMOBILIZATIONANDITSCHARACTERISTICSOFMETHANOZATIONTianShen1,ZhaoJun1,CaoYali1,LuoHaipeng1,WuChuangzhi2,YuanZhenhong2,YangXiushan1(1.DepartmentofBiology,CapitalNormalUniversity,Beijing100037,China;2.InstituteofEnergyResources,ChinaAcademyScience,GuangZhou510070,China)Abstract:AcombinedtechniqueofadsorptionandentrapmentwasconductedtoimmobilizeMethanosarcinatoovercometheshortcomingsinimmobilizedmethanosarcinausingPVAastheentrapmentmatrix.Thecharacter2isticsoftheimmobilizedMethanosarcinaweredemonstratedusingUASBreactorwithdefinedstrengthorganicwastewaterandsoybeancakewastewater.TheresultsindicatedthatthehighestCODloadingrate,thehighestCODremovalrate,theshortesthydraulicretentiontime,andthemethanecontentwerereachedupto14.7kgCOD/(m3d),94.3%,16.4hand65%73%,respectivelyforthedefinedwastewater.ThehighestCODloadingrateof17.6gCOD/(m3d),theaverageCODloadingrateof8.2kgCOD/(m3d),theshortesthy2draulicretentiontimeof13.7h,thehighestbiogasproductionrateof7L/(dL),thehighestCODremovalrateof87.0%andtheaverageCODremovalrateof70.4%forsoybeancakewastewaterwereaccomplished.TheaveragebiogasproductionrateandCODremovalratewere15.2and1.2timeshigherthanthatofunimmobi2lizedoneintheperformanceofsoybeancakewastewatertreatment.Goodprecipitatinganddegassingofimmobi2lizedMethanosarcinabythecombinedtechnologyofadsorptionandentrapmentwereobtained.Keywords:adsorptionandentrapment;immobilizedMethonasarcina;methanozationEmail:xsyang@mail1cnu1edu1cn1876:©1995-2005TsinghuaTongfangOpticalDiscCo.,Ltd.Allrightsreserved.