产甲烷菌在厌氧消化中的应用研究进展

林代炎1,林新坚2,杨菁1,叶美锋1(1.,350003;2.,350013):2007-07-26;2007-12-21:(1963-),,,(Email:lindaiyan@126com):(1576);(STIF-Y01):,:;;:X703:AAdvanceinutilizationofmethanobacteriaforanaerobicdigestionstudiesLINDaiyan1,LINXinjian2,YANGJing1,YEMeifeng1(1.AriculturalEngineeringInstitute,FujianAcademyofAgriculturalSciences,Fuzhou,Fujian350003,China;2.SoilandFertilizerInstitute,FujianAcademyofAgriculturalSciences,Fuzhou,Fujian350013,China)Abstract:Thisarticlebrieflyintroducestheprogressinusingmethanobacteriaforanaerobicdigestionstudies.Italsoanalyzestherelationshipbetweentheresearchdevelopmentinanaerobicdigestionandthemetabolicmechanismandthephysiologicalandbiochemicalcharacteristicsofmethanobacteria.Keywords:anaerobicdigestion;methanogensbacteria;anaerobicreactor,,,,,,,,1产甲烷菌研究历史1899,,,,,(Methanobacillusomelauskii),1950,Hungate[1-2],,8,2070,1(),39,1991,(Oregon)2153655,[3],,5,(Methanohacteriales)(Methanococcales)(Methanosarcinales)(Methanomicrobiales)(Methanopyrales)[4],200[5],,smallsubunitribosomalRNA;19961福建农业学报23(1):106~110,2008FujianJournalofAgriculturalSciences:1008-0384(2008)01-0106-05Methanococcusjannaschii,45[5],,,3[1];,CNP,,,K+Na+Ca2+Co2+Cl-Fe2+Fe3+[5],,,,Hungate[1],,2产甲烷菌的代谢机理及生理生化特征3,60%;30%,70%[6-7];10%,,ATPATP11,3,9[2],[8],7,D,[9-10],,,,,[11],,-600~-560mV;-350~-300mV[12]5~83,:3555[13]pH65~75,,,7551[12]3产甲烷菌在厌氧消化中的应用31,,,,,,[8],,Chosh2070,(TwophaseBiosystem,),,,,,[14-16],,,,,,[17-19],,[2],,SO2,32,,,,,,,,,H2/CO2,[20-21],1071林代炎等:产甲烷菌在厌氧消化中应用研究进展[22],,70%[6-7],,(),()[7,23],,300mgL-1,3~5[24],31;2;3,(AF),,[25];,,(UASB);[26],,,,,,,UASB,,33331接种产甲烷菌,,;,,,[27],,,,,,,[28],,[29],[30]332调节消化污水的营养物质平衡,,,,Na+K+Ca2+,,,,,,;,Speece,,,[31],VFA,,,,[32-35],FeCoNi10mgL-1d-101mgL-1d-102mgL-1d-1[36],[21],,,,[37];,Hormesis[38];La3+Ce3+VFA[39],CNPK,,,CNP,,CNP7551,CCOD,CODNP20051,COD300,,,,100~150gL-1[40],108福建农业学报23[41]Kunna,COD/N53,N2,COD/N53,[42],:,VFA[43-45],CC/N,333pH值对产甲烷菌的影响pH,pH,pHpH,,pH72~76,COD13,,[46],1000mgL-1CaCO3,,,Ca2+Mg2+CO2-3SO2-4,334毒性物质控制与菌种驯化,,,,SO2-4,S2-,[47],,,,Karhadkar[48],,Parking[49]50mgL-1Isa[50]20mgL-1500mgL-1,,,,3结语,,,,,,,;,,,,:[1]HUNGATERE.Arolltubemethodforcultivationofstrictanaerobes[M]//NORRISJRMethodsinMicrobiology.NewYork:AcademicPress,1969.[2]GARCIAJL,PATELKC,OLLIVIERBTaxonomic,phylogefletieandecologicaldiversityofmethanogenic[J].AmhAnaerobe,2000(6):205-226.[3],.[J].,1991,18(3):169,187.[4]LANGEM,AHFINGBKAcomprehensivestudyintothemolecularmethodologyandmolecularbiologyofmethanogenic[J].ArchaeaFEMSMicrobiolRev,2001,5(12):553-571.[5],,.[J].,2003,23(6):42-46.[6]MCCARTYPL,SMITHDPAnaerobicWastewaterTreatment[J].EnvironmentalSciTeChnol,1986,20(12):1200-1206.[7]HARPERSR,POHLANDFG.Recentdevelopmentsinhydrogenmanagementduringanaerobicbiologicalwastewatertreatment[J].BiotechBioeng,1986,28:585-602.[8].[J].,1992(4):1-4.[9],,.[J].,1997(2):3-6.[10].[J].,l990,13(6):83-87.[11].[J].,1990,8(2):5-8.[12],,,.[M].:,2004:96-103.[13],,,.[M].:,2005:24-53.[14]COHENA,BREUREAM,VANANDELJG,etalInfluenceofPhaseSeparationontheAnaerobicDigestionofGlucoseStabilityandKineticResponsestoShockLoadings[J].WstRes,1982,16:449-455.[15]COHENAInfluenceofPhaseSeparationontheAnaerobicDigestionofGlucose,MaximumCODTumoverduringContin1091林代炎等:产甲烷菌在厌氧消化中应用研究进展uousOperation[J].WatRes,1980,14:1439-1448.[16]COHENAAnaerobicDigestionofGlucosewithSeparatedAcidProductionandMethaneFormation[J].WatRes,1979,13:571-580.[17],.[J].,1998,19(6):56-59.[18],,.[J].,1999,17(4):3-6.[19],.[J].,2000,18(2):3-7.[20],.[J].,1997,35(5):378-384.[21],,,.[J].,1994,13(1)59-63.[22]WANG,SYYINGC.AraerobicTreatmentaGrowthopTechnolWaterTreatmentConference15-19Sept1986,RIAHailsAmsterdam[J].TheNetherlands,1986.[23]BRUMMELERET,POLWH,DOLFINGJ,etal.MethanogenesisinanUpflowAnaerobicSludgeBlanketReactoratpH6onanAcetate-PropionateMixture[J].ApplEnvironMicrobiol,1985,49(6):1472-1477.[24],[J].,2000,18(2):8-11.[25],ANDRESTILCHE.[J].,1989,29(2):145-148.[26][J].,1999,17(2):3-6.[27],,UASB[J].,2000,18(2):17-31.[28],,[J].,1998,29(4):330-333.[29],[J].,1994,12(1):1-5.[30],,[J].,2002,23(5):675-677.[31]SPEECERE.Naerobicbiotechnologyforindustrialwastewater[M].TNUSA:ArchaePress,1996:222-226.[32][J].,l996,14(1):1-5.[33]FANGHH,HKCHUI,LIYAXINMicrobialstuctureandactivityofUASBgranulestreatingdifferentwastewater[C].//7thInternationalSymposiaAnaerobicDigestion,SouthAfrica,1994.[34]LIYAXIN,RESPECCEStimulationeffectoftracemetalsonanaerobicdigestionhighsodiumcontentsubstrate[J].WaterTreatment,1995,10(2):145-154.[35]TAKASHIMAM,SPEECEREMineralnutrientrequirementforhighfatemethanefermentationofacetateatlowSRT[J].ResJWatPollControlFed,1989,61(11/l2):1646-1650.[36],[J].,2001,23(3):116-118.[37],,,.[J].,1994,14(4):439-444.[38],,,.Hormesis[J].,2004,14(6):82-84.[39],,,.La3+,Ce3+VFA[J].,2007,25(3):3-6.[40]COONEYCL.Growthofmicroorganism//rechmHJReedG(eds)BiotechnologyAcomprehensivetreatisein8volumesVol1:Microbialfundameatals[C].VerlagChemieWeinheimDeerfieldBeachForidaBasel,73.[41],,[J].,2006,37(6):838-841.[42]AKUNNAJC,BIZEAUC,MOLETTAR.Denitrificationinanaerobicdigesters:possibilitiesandinfluenceofwastewaterCOD/N-NOxratio[J].Enviro