28520085ActaScientiaeCircumstantiaeVo.l28,No.5May,2008:(No.5040821);(863)(No.2007AA062347)SupportedbytheNationalNaturalScienceFoundationofChina(No.5040821)andtheHigh-TechResearchandDevelopmentProgramofChina(No.2007AA062347):(1982)),;*(),Te.l&Fax:(010)62849108;E-mai:lyswe@ircees.ac.cnBiography:ZHUHui(1982)),male;*Correspondingauthor,Te.l&Fax:(010)62849108;E-mai:lyswe@ircees.ac.cn,,,.2008.[J].,28(5):910-915ZhuH,WeiYS,WangYW,etal.2008.EffectofTubificidaeonactivatedsludgesettlingperformance[J].ActaScientiaeCircumstantiae,28(5):910-915诸晖,魏源送*,王亚炜,刘俊新中国科学院生态环境研究中心,北京100085:2007-03-22:2007-06-27:2008-01-24:3(TSSU4g#L-16g#L-18g#L-1),,.,60min,,60min8%~42%;,.,,(1~3h)Zeta;(SVI)(EPS).:;;;:0253-2468(2008)05-910-06:X703:AEffectofTubificidaeonactivatedsludgesettlingperformanceZHUHu,iWEIYuansong*,WANGYawe,iLIUJunxinResearchCenterforEco-EnvironmentalSciences,ChineseAcademyofSciences,Beijing100085Received22March2007;receivedinrevisedform27June2007;accepted24January2008Abstract:RecentlyTubificidae(Oligochaete:Tubificidae)hasbeenobservedtohavepotentialtoimprovesludgesettlinganditiswidelyappliedinthestudyofsludgereduction.ThepurposesofthisstudywerethereforetosystematicallyinvestigatetheeffectonsludgesettlingperformanceinducedbyTubificidae.ThewormswereinoculatedatvariousadditionratiosintosludgeofdifferentTSSconcentrations(TSSU4g#L-1,6g#L-1&8g#L-1)andtheresultingsludgecharacteristicsweremeasuredtounderstandtheeffectsofTubificidaeaddition.Resultsshowedthatarapidanddramaticenhancementofsludgesettlingratewasobservedwithin60minutesafterTubificidaeaddition,especiallyforsludgewithhighTSSconcentration.ComparedwiththesludgewithoutTubificidae,thesludgevolumeatvariousTSSconcentrationscanbereducedby8%~42%within60minafterworminoculation.SludgecharacterizationstudiesshowedthatthemeanflocsizeandZetapotentialofactivatedsludgechangedlittleafteradditionofTubificidae,thoughSVIandextra-cellularpolymericsubstances(EPS)concentrationswereslightlyincreasedwithinashort-termafterworminoculation.Keywords:activatedsludge,settlingperformance,sludgecharacteristics,Tubificidae1(Introduction),.,,,(Elissenetal.,2006;Ghyootetal.,2000;Huangetal.,2007;Lapinsk,i2003;Leeetal.,1996a,1996b;,2004;Liangetal.,2006a,2006b;Liuetal.,2001;Luxmyetal.,2001;Ratsaketal.,1993,1994,2006;Rensinketal.,1997;Weietal.,2003a,2003b,2005,2006).,(Leeetal.,1996a,1996b;.2004;Liang,2006a,2006b;Rensinketal.,1997;Weietal.,2003b,2005,2006);,MLSS(Luxmyetal.,2001).,,.,((Aeolosomahemprichici)(Naiselinguis)),(5:(Tubificidae)),(Elissenetal.,2006;,2004;Liangetal.,2006b;Weietal.,2003b).,.,(Mikkelsenetal.,1996).,,,,.,,,.2(Materialsandmethods)2.1实验方法(SVI100)(SVI100).(TSS)3(TSSU4g#L-16g#L-18g#L-1).,(/)1,100mL,,SV30SV60,TSS,SVI.3,2,2.,(Limnodrilushoffmeisteri),(Branchiurasowerbyi)(Tubifextubifex),1~2.,,.3,1200mL,.0h1h3h,40,,TSS(TotalSuspendSolids)SVI(SludgeVolumeIndex)EPS(Extra-celluarpolymericsubstances).Zeta,TSS=7.4g#L-1TSS=10.0g#L-12,1,30min1h2h3hEzeta,Ezeta.2.2分析方法(SVI)30min60min(SV30SV60).(TSS):,10mL,0.45Lm,105e2h,,(g)(g#L-1).:MalvernMASTERSIZER2000.(EPS):EPS(Liuetal.,2002).10mL50mL,0106mL(36.5%),4e1h.4mL(1mol#L-1)NaOH,4e3h.4e10000r#min-1(20000g)20min.,0122Lm,EPS.-(Raoetal.,1989),.Zeta:,30min1h2h3h,MalvernZETASIZER2000ZetaEzeta.3(Results)3.1颤蚓改善活性污泥沉降性能的效果,3TSS4.39g#L-15.99g#L-17.72g#L-1.3TSS,1.1.SV(),min91128.,SV30SV60SVI,60min;,,,;,.1Table1EffectonsludgesettlingperformanceinducedbyTubificidaeTSS/(g#L-1)SV30SV60SVI/(mL#g-1)SludgeÑ4.3926.5%24.3%8.5%24.0%22.0%8.3%60.455.28.5%SludgeÒ5.9960.0%46.0%23.3%41.0%34.0%17.1%100.279.823.3%SludgeÓ7.7294.5%74.0%21.7%88.0%51.0%42.0%122.495.921.7%1vFig.1Sludgesettlingcurveandsettlingvelocity(v)atvariousTSSconcentrations2SVIFig.2EffectofTubificidaeonSVIofactivatedsludge3.2颤蚓对活性污泥特性的影响3.2.1SVI1h3h,403,SVI,2.SVI,0hSVI.,3,SVI,(4.395.99g#L-1),3hSVI.,,,,,.3.2.2胞外聚合物(EPS)33,EPS().3,EPS,,EPS.,9125:,,3h,.3Fig.3EffectoftubificidaeonEPSconcentrations(includingProtein&Polysaccharide)ofactivatedsludge3.2.3污泥平均粒径TSS4.39g#L-1,4.,3h,,.4Fig.4EffectofTubificidaeonmeanflocsizeofactivatedsludge3.2.4Zeta电位(Ezeta)Zeta.2(TSS=7.435g#L-1,SVI=3510TSS=10.01g#L-1,SVI=96.4),(+)(+),30min1h2h3hEzeta,1.5,,Zeta,Zeta3,Zeta.913285ZetaFig.5EffectofTubificidaeonZetapotential4(Discussion).,(SVIEPSZeta),,,.,SVIEPS3h.EPS,,.EPS(,2004).,EPSSVI(,2003;,2004).EPS,3h,SVI;,,.,,,,;,EPS.,,7.72g#L-1,3hEPS7.4%.,EPSSVI..,,.30min,(1),TSS.,TSS.1,.4,(Lagstage)(Transitionstage),(Zonesettlingstage)(Compressionsettlingstage)(,2006;Metcalf&EddyInc.,2003).,,.,(TSS=7.72g#L-1),.,,,,.,,,.,.,.,70%(,2000)..,,,,..9145:5(Conclusions)1),..2)(SVIEPSZeta),.3),.:魏源送(1969)),男,博士,副研究员;Te:l010-62923543;E-mai:lyswe@ircees.ac.cn.主要从事污水生物处理污泥处理等方面的研究工作,主持和承担多项国家863课题国家水专项国家自然科学基金项目及地方科研项目等,已在国内外刊物上发表论文20多篇.ReferencesElissenHJH,HendrickxTLG,TemminkH,etal.2006.Anewreactorconceptforsludgereductionusingaquaticworms[J].WaterResearch,40:3713)3718GhyootW,VerstraeteW.2000.Reducedsludgeproductioninatwo-stagemembrane-assistedbioreactor[J].WaterResearch,34(1):205)215HuangX,LiangP,QianY.2007.ExcesssludgereductioninducedbyTubifextubifexinarecycledsludg