常温下亚硝化活性污泥的驯化及其特征周玲君

12121212121．4100822．410082SBR。SBR15～30℃110d8h5h、3h1．2～1．4mg/L。0．24kgNH+4－N/m3·d80%90%。NO－2。EPSTB－EPSLB－EPS。Ze-ta。。EPSX703A1000－4602201107－0010－0530970105K0802151－312009ZX07212－001－02DomesticationandCharacteristicsofShort-cutNitrificationActivatedSludgeatNormalTemperaturesZHOULing-jun12YANGZhao-hui12LUOYuan-ling12XUZheng-yong12WANGLi-ke121．CollegeofEnvironmentalScienceandEngineeringHunanUniversityChangsha410082China2．KeyLaboratoryofEnvironmentalBiologyandPollutionControl＜MinistryofEducation＞HunanUniversityChangsha410082ChinaAbstractInordertoimprovenitrogenremovalperformanceshort-cutnitrificationactivatedsludgewascultivatedinSBRfor110daysatnormaltemperatures15to30℃．TheoperationcycleofSBRwas8hourswithaerationof5handnon-aerationof3h．TheDOwascontrolledat1．2to1．4mg/Linintermittentaerationphase．Theresultsshowthattheinfluentloadingrateofshort-cutnitrificationprocesscanreach0．24kgNH+4－N/m3·dandtheremovalrateofammonianitrogenandthenitro-sationrateareover80%and90%respectively．TheproblemsoflowsludgeconcentrationandinstabletreatmentefficiencyinthereactorduetoNO－2－Naccumulationareresolvedbyreplacingsludgesuperna-tantoncepersemi-month．TheanalysisoftheextracellularpolymericsubstancesEPSindicatesthatthecontributionofTB-EPStosludgesettleabilityishigherthanthatofLB-EPS．TheZetapotentialofthedo-mesticatedsludgeisdecreasedindicatingthatthesettleabilityofsludgeinshort-cutnitrificationprocess·01·27720114CHINAWATER＆WASTEWATERVol．27No．7Apr．2011isbetter．Inadditionaftertheshort-cutnitrificationactivatedsludgeissuccessfullydomesticatedthereactorcanbestablyoperatedathighloadingrate．KeywordsSBRshort-cutnitrificationprocessextracellularpolymericsubstancesEPS25%40%1。/。AOBNOBDO、、2。NO－2－N/NO－x－N3。NO－2NO－2－N4。AOB。11.1SBR1。1Fig．1SchematicdiagramofexperimentalequipmentSBR150mm400mm5L。。15～30℃pH7～8。SBR8h4。0～94d75mL1500mL25mL500mL95～110d1500mL/d5h、3hDO1．2～1．4mg/L0．5h。1.21NH+4－N800mg/LC/N3∶1N/P20∶1。VSS/SS=0．58SVI183mL/gMLSS3589mg/L。1Tab．1Componentsofsyntheticwastewatermg·L－12544FeSO4·7H2O0．0100CaCl2·2H2O0．1800NH42SO43768CuSO4·5H2O0．0032MgSO4·7H2O0．3000KH2PO488ZnSO4·7H2O0．0044MnCl2·4H2O0．0102NaHCO33500CoCl2·6H2O0．0033NiCl2·6H2O0．0190EDTA0．01001.3NH+4－NNO－3－NNO－2－NN－1－－MLSSpHpHDOAOBNOBOUR5。6EPSEPSTB－EPSLB－EPSEPS。、Folin·11·．watergasheat．com277EPS、EPS。EPSNanoZS90ZetaZeta。22.111SBR28%247。DONOB。79d30%0．24kgNH+4－N/m3·dNO－2－N20～35mg/LNO－3－N5mg/LNH+4－N80%9490%。2、Fig．2Variationsofwaterexchangeratenitrogenconcentrationineffluentandnitrosationratewithtime2.22.2.130MLSS3589mg/L1668mg/L3①NO－2②8③C/N。30500mLMLSS2024mg/L71%。MLSS2000mg/L0．02kgNH+4－N/m3·d11LMLSS。。50、70、80MLSS2053、2282、2467mg/L。9。3MLSSSVIFig．3MLSSandSVIvscultivatingtime759070．66%NO－2－N。30min500mL80mg/L。4d90%。SVI90110mL/g11044mL/g。1、2、3123。。2.2.2。AOB0．3298mgO2/gMLSS·min0．8412mgO2/gMLSS·minNOB0．4127mgO2/gMLSS·min0．1876mgO2/gMLSS·min。AOBNOBAOB。AOB10NOB。3。2.2.3EPSEPSLB－EPS、·21·277．watergasheat．comTB－EPS。EPS2。EPS、11EPS。2EPSTab．2FT-IRspectroscopyresultofextracellularpolymericsubstances/cm－13600～3200O—H3000～2800C—H3000O—H1720CO1440～1390C—O、O—H1650COC—N、N—H1460—CH2—、—CH31160～1130C—O—C930～760C—H—CH2——CH2—4EPSm/m。TB－EPS。EPSTB－EPS。EPS1213EPS。4EPS/Fig．4ContentsofproteinandpolysaccharideinLB-EPSandTB-EPS、EPSLB－EPS14。TB－EPS。Liao15ZetaMLSS、SVI。LB－EPS、TB－EPS3LB－EPSTB－EPSSVI。TB－EPSLB－EPS。TB－EPSm/mLB－EPS16TB－EPSTB－EPSLB－EPS。。3LB－EPSTB－EPSZetaTab．3ZetapotentialofsludgebeforeandafterextractionofLB-EPSandTB-EPSmVLB－EPSTB－EPS/d0－25．9－26．4－38．546－20．6－20．2－36．494－16．3－20．9－33．83①90%AOB。②、。。③。④TB－EPSLB－EPS。1VerstraeteWPhilipsS．Nitrification-denitrificationprocessesandtechnologiesinnewcontextsJ．Environ·31·．watergasheat．com277Pollut1998102s1717－726．2XuZYZengGMYangZHetal．Biologicaltreatmentoflandfillleachatewiththeintegrationofpartialnitrifi-cationanaerobicammoniumoxidationandheterotrophicdenitrificationJ．BioresourTechnol2010101179－86．3GuoJHPengYZWangSYetal．Effectiveandro-bustpartialnitrificationtonitritebyreal-timeaerationdurationcontrolinanSBRtreatingdomesticwastewaterJ．ProcessBiochem2009449979－985．4BlackburneRYuanZGKellerJ．Demonstrationofni-trogenremovalvianitriteinasequencingbatchreactortreatingdomesticwastewaterJ．WaterRes2008428/92166－2176．5．OURJ．1999193225－229．6．MBRJ．200925580－83．7YangQPengYZLiuXHetal．Nitrogenremovalvianitritefrommunicipalwastewateratlowtemperaturesu-singreal-timecontroltooptimizenitrifyingcommunitiesJ．EnvironSciTechnol200741238159－8164．8．J．200728112462－2466．9MikkelsenLHKeidingK．Physico-chemicalcharacter-isticsoffullscalesewagesludgeswithimplicationstode-wateringJ．WaterRes200236102451－2462．10．J．200646122077－2080．11BalaSSYanSTyagiRDetal．Extracellularpolymer-icsubstancesEPSproducingbacterialstrainsofmu-nicipalwastewatersludgeIsolationmolecularidentifi-cationEPScharacterizationandperformanceforsludgesettlinganddewateringJ．WaterRes20104472253－2266．12WilénBMLumleyDMattssonAetal．RelationshipbetweenfloccompositionandflocculationandsettlingpropertiesstudiedatafullscaleactivatedsludgeplantJ．WaterRes200842164404－4418．13．D．2008．14YangSFLiXY．InfluencesofextracellularpolymericsubstancesEPSonthecharacteristi