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1231．3521002．3521003．201620、。6%1∶650℃4h65．72%。6%1∶450℃5h62．63%。。X703A1000－4602201401－0022－04ExtractionofProteinfromExcessSludgebyCombinationofUltrasonicandBiologicalTreatmentLIPing1LEIYing2LIDeng-xin31．DepartmentofChemistryNingdeNormalUniversityNingde352100China2．NingdeEnvironmentalMonitoringStationNingde352100China3．CollegeofEnvironmentalScienceandEngineeringDonghuaUniversityShanghai201620ChinaAbstractExcesssludgewashydrolyzedbycombinationofultrasonicandalkalineproteaseorpa-pain．Theinfluenceoftwodifferentenzymesonsludgetreatmenteffectwasinvestigatedbyproteinextrac-tionunderultrasonicpretreatment．Theresultsshowedthattheoptimumconditionsofalkalineproteasehydrolysiswereproteasecontent6%theratioofsolidtoliquid1∶6temperature50℃andreactiontime4h．Undertheseconditionstheproteinextractionratewas65．72%．Theoptimumconditionsofpapainhydrolysiswerepapaincontent6%theratioofsolidtoliquid1∶4temperature50℃andre-actiontime5h．Theproteinextractionrateherewas62．63%．Thecombinationofultrasonicandalka-lineproteasehadanadvantageoverultrasonic-papainforextractingproteinfromexcesssludge．Keywordsultrasonicproteaseexcesssludgehydrolysisprotein2011H203、2012H206JB1019320122012015212。。、、·22·30120141CHINAWATEＲ＆WASTEWATEＲVol．30No．1Jan．2014。、34。Tiehm5。Chiu6SCOD/TCOD36%89%。。11.1。2．0×104m3/dCarrousel2000。pH=6．4385．56%38．42%。1.2HCl、NaOH、、H2SO4、CuSO4·5H2O、K2SO4、、。。1.31.3.110g100mL40mL1∶4。10、20、30、40、50min10、20、40、60、80W。1.3.2。。10min4000r/min10min。、、4L9341。1Tab．1Factorsandlevelsoforthogonalexperimentsofproteasehydrolysis/%/℃/h145031∶4255541∶6366051∶81.4。。LρPr=VHCl×MHCl×0．014×6．25mDS×100%1L=WPrVlmρPr×100%2VHCl———MHCl———mDS———ρPr———Vl———m———WPr———22.12.1.11。1Fig．1Changeofproteinextractionratewithultrasonictime10～20min20～40min。·32·．watergasheat．com301。40min14．89%。。。。40min。2.1.22。2Fig．2Changeofproteinextractionratewithultrasonicpower210～20W20～40W40W40W13．57%。＞40W。740W。2.22。65．72%40．86%24．86%62．63%38．15%24．48%。4。1∶6。。。1∶4。2Tab．2Ｒesultoforthogonalexperimentsofproteasehydrolysis/%/℃/h/%/%145031∶457．2152．20245541∶659．8445．60346051∶840．8640．81455531∶842．4538．15556041∶458．3754．90655051∶664．1361．11766031∶661．7661．61865041∶865．7248．33965551∶458．4562．63152．63762．35353．80758．010254．98353．58061．31061．910361．97753．66354．48049．677Ｒ9．3408．7737．50312．2331'46．20353．88050．65356．5772'51．38748．79349．61056．1073'57．52352．44054．85042．430Ｒ'11．3205．0875．24014．1476%。。。1∶66%50℃4h1∶46%50℃5h。2.3、3。·42·301．watergasheat．comPb、Cr、Cd、HgAsCu、Ni、Zn。《》GB13078—2001。3Tab．3Effectcomparisonofsludgeproteinextractionbycombinationofultrasonicandtwoenzymehydrolysises/%/mg·kg－1CuNiZn14．890．030．060．0165．720．080．230．0462．630．040．190．02Pb、Cr、Cd、Hg、As0．001mg/kg。3。。8。3①40min、40W。1∶66%50℃4h1∶46%50℃5h。②65．72%62．63%。Pb、Cr、Cd、Hg、AsCu、Ni、Zn。1．J．200613262－65．2．J．20034144－52．3．J．20083683344－3345．4．J．2008284726－730．5TiehmANickelKZellhornMetal．UltrasonicwasteactivatedsludgedisintegrationforimprovinganaerobicstabilizationJ．WaterＲes20013582003－2009．6ChiuYCChangCNLinJGetal．Alkalineandultra-sonicpretreatmentofsludgebeforeanaerobicdigestionJ．WaterSciTechnol19973611155－162．7．J．20082941002－1006．8．J．200526168－71．1979－、。E－maillp993@126．com2013－09－15·52·．watergasheat．com301