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翟志军1,2,马欢1,李军1,蔡冬清1,王相勤1,吴跃进1,姚建铭1,2*,余增亮1(1.,230031;2.,230088)[目的]探讨对巢湖蓝藻厌氧发酵资源化利用的潜力[方法]以巢湖新鲜蓝藻为原料,进行厌氧发酵产沼气试验,分析产沼气的最佳条件[结果]结果表明,接种物与蓝藻体积比为12时,产气最佳在平均温度为27.5的发酵环境中发酵50d,蓝藻TS产气潜力为368.25ml/g,VS产气潜力为383.33ml/g,沼气中甲烷的平均含量为63.46%,蓝藻TS利用率为54.01%,VS利用率为58.35%[结论]巢湖新鲜蓝藻可以作为发酵原料生产沼气巢湖;蓝藻;厌氧发酵;沼气S216.2A0517-6611(2008)12-05084-02StudyontheProductionBiogaswithBlueAlgaefromChaohuLakeZHAIZhijunetal(KeyLaboratoryofIonBeamBioengineering,ChineseAcademyofSciences,Hefei,Anhui230031)Abstract[Objective]ThepotentialofChaohubluealgaeresourceutilizationbyanaerobicfermentationwasdiscussed.[Method]WithChaohufreshbluealgaeastestmaterials,theexperimentofproducingbiogasbyanaerobicfermentationwascarriedout,andtheoptimalconditionsofproducingbiogaswereanalyzed.[Result]Theresultshowedthattheoptimalconditionofproducingbiogaswereasfollows:thevolumeratioofinoculumtobluealgaewas12.Theanaerobicfermentationlastedfor50daysattheaveragetemperatureof27.5,andthepotentialofproducingbiogasofTSandVSwas368.25and383.33ml/g,respectively.Theaveragecontentofmethaneinthebiogaswas63.46%,theutilizationpercentageofTSandVSwas54.01%and58.35%,respectively.[Conclusion]Chaohufreshbluealgaecanbeusedasrawmaterialstoproducebiogas.KeywordsChaohulake;Bluealgae;Anaerobicfermentation;Biogas中国科学院创新项目(KSCX2SW324)翟志军(1982-),男,安徽长丰人,硕士研究生,研究方向:环境与资源微生物学*通讯作者200802252080,,90,,,[1]21,5~10,1m,,,[2],6~8mg/L,50~70t()[3],,,,,[2,4-5],11.1,,40%(V/V),,(Totalsolids,TS)1.83%,(Volatilesolids,VS)92.59%TS=9.63%,VS=63.55%1.2,,,pH,:GC14C:500ml,,,1.31.3.1TSVSpH,(1052),(55010),[6]pHpH1.3.2[7]1~4dGC14C;:Ar;Porapakq:40;50;:50,60mA:Y=1.07505+0.00029x,r2=0.999411.3.312,3,31,12311.51213,33.0,22.0,27.550d22.1TSVSpH111TSVS11.88%5.43%,213.03%5.13%,312.83%5.67%TSVSTSVS,1TSVSpHTable1TS,VSandpHvaluebeforeandafterfermentationTreatmentBeforetreatmentTS%VS%pHAftertreatmentTS%VS%pH15.0568.887.64.4565.147.5Experimentalgroup113.5466.317.43.5164.547.5Controlgroup124.7671.897.74.1468.727.5Experimentalgroup223.3463.257.43.2961.347.5Controlgroup233.8274.037.83.3369.837.5Experimentalgroup333.1359.877.33.0158.987.5Controlgroup3,JournalofAnhuiAgri.Sci.2008,36(12):5084-5085,5087李玮况玲玲2.250d,121,,6~8d,1,6;3,8d,,112ml/d;21,33030ml/d,78%,;30,,20%,,,2,(30%),,3433%,10,12(60%~72%);21;3(56%~66%):(20~30)1,(61),,[8-9],,,,1Fig.1Changeofbiogasproduction2Fig.2Changetrendofmethanecontent2.31,12,(2)2,1,1539ml,,65.12%;3,;2,1151.8ml,11002.2ml31119.8ml,TS54.01%,VS58.35%,,12,,1/20,,,2Table2ConditionofbiogasproductionTreatmentmlTotalbiogasproduction%AveragemethanecontentTSml/gGasproductionrateofTSVSml/gGasproductionrateofVS1153965.12344.54358.63Experimentalgroup11----Controlgroup12181563.46368.25383.33Experimentalgroup22----Controlgroup23195557.28384.21399.94Experimentalgroup33----Controlgroup3:-,Note:-standsforfew,therefore,hasnostatisticalsignificance.3,27.5,12,TS368.25ml/g,VS383.33ml/g,63.46%,50t(),1.8m3,,,,,[10],,,,,,,[1]何开丽.巢湖富营养化现状与治理对策[J].环境保护,2002(4):22-24.[2]程真文.巢湖藻类污染的研究和对策[J].水处理技术,2003,29(4):247-248.[3]蒋雪英.巢湖水华的形成和防治对策[J].内陆水产,2000,12(38):38-39.(下转第5087页)508536卷12期翟志军等巢湖蓝藻产沼气的试验研究,,1Fig.1Relationshipbeweengaseouspollutantsconcentrationandaltitude2.1.21,NO2-0.85,,4-0.996,3,NO2(126~165/h,),,,,,,NO2NO2,(0.010mg/m3),,NO2,,,,2.22.2.12,5SO2,,,,,,,2.2.23,53NO2,(r=0.82~0.86),3,,,2,NO2,NO2,,()NO2,,2Fig.2ChangecurveofSO2concentration3Fig.3ChangecurveofNO2concentration3,,,(),,,,,,,,,,[1]国家环境保护总局.环境空气质量自动监测技术规范(HJ/T1932005)[S].北京:中国环境科学出版社,2006.(上接第5085页)[4]谢萍,周学文,杨家雄,等.滇池蓝藻对肉仔鸡和生长肥育猪饲用效果研究[J].云南畜牧兽医,2000(2):12-14.[5]董诗旭,董锦艳,宋洪川,等.滇池蓝藻发酵产沼气的研究[J].可再生能源,2006(2):16-18.[6]中科院成都生物研究所.沼气发酵常规分析法[M].1981:1-90.[7]JUHAKALLEM,EINOLARIITTAH,ETTUNENJUKKAA,etal.Responsesofmethaneoxidationtotemperatureandwatercontentincoversoilofaboreallandfill[J].SoilBiology&Biochemistry,2007(39):1156-1164.[8]边义,刘庆玉,李金洋.玉米秸秆干发酵制取沼气的试验[J].沈阳农业大学学报,2007,38(3):440-442.[9]HONGWEIYEN,DAVIDE.Anaerobiccodigestionofalgalsludgeandwastepapertoproducemethane[J].BioresourceTechnology,2007,98:130-134.[10]蔡冬清,柳丹,季程晨,等.磁控改性复配物治理水华的研究[J].中国环境学,2007,27(5):661-664.508736卷12期鲍雷等重庆缙云山气态污染物梯度对比监测及分析