翟志军1,2,马欢1,李军1,蔡冬清1,王相勤1,吴跃进1,姚建铭1,2*,余增亮1(1.,230031;2.,230088) [目的]探讨对巢湖蓝藻厌氧发酵资源化利用的潜力。[方法]以巢湖新鲜蓝藻为原料,进行厌氧发酵产沼气试验,分析产沼气的最佳条件。[结果]结果表明,接种物与蓝藻体积比为1∶2时,产气最佳。在平均温度为27.5℃的发酵环境中发酵50d,蓝藻TS产气潜力为368.25ml/g,VS产气潜力为383.33ml/g,沼气中甲烷的平均含量为63.46%,蓝藻TS利用率为54.01%,VS利用率为58.35%。[结论]巢湖新鲜蓝藻可以作为发酵原料生产沼气。 巢湖;蓝藻;厌氧发酵;沼气 S216.2 A 0517-6611(2008)12-05084-02StudyontheProductionBiogaswithBlueAlgaefromChaohuLakeZHAIZhi-junetal (KeyLaboratoryofIonBeamBioengineering,ChineseAcademyofSciences,Hefei,Anhui230031)Abstract [Objective]ThepotentialofChaohubluealgaeresourceutilizationbyanaerobicfermentationwasdiscussed.[Method]WithChaohufreshbluealgaeastestmaterials,theexperimentofproducingbiogasbyanaerobicfermentationwascarriedout,andtheoptimalconditionsofproducingbiogaswereanalyzed.[Result]Theresultshowedthattheoptimalconditionofproducingbiogaswereasfollows:thevolumeratioofinoculumtobluealgaewas1∶2.Theanaerobicfermentationlastedfor50daysattheaveragetemperatureof27.5℃,andthepotentialofproducingbiogasofTSandVSwas368.25and383.33ml/g,respectively.Theaveragecontentofmethaneinthebiogaswas63.46%,theutilizationpercentageofTSandVSwas54.01%and58.35%,respectively.[Conclusion]Chaohufreshbluealgaecanbeusedasrawmaterialstoproducebiogas.Keywords Chaohulake;Bluealgae;Anaerobicfermentation;Biogas 中国科学院创新项目(KSCX2-SW-324)。 翟志军(1982-),男,安徽长丰人,硕士研究生,研究方向:环境与资源微生物学。*通讯作者。 2008-02-25 2080,,90,,,[1]。21,5~10,1m,,,[2]。,6~8mg/L,50~70t()[3]。,,、,。、,、,[2,4-5]。,。1 1.1 ,,40%(V/V),。,(Totalsolids,TS)1.83%,(Volatilesolids,VS)92.59%。。TS=9.63%,VS=63.55%。1.2 ,,,pH,:GC-14C。:500ml,,,。。1.3 1.3.1 TS、VSpH。、,(105±2)℃,(550±10)℃,[6]。pHpH。1.3.2 [7]。1~4dGC-14C;:Ar;Porapakq:40℃;50℃;:50℃,60mA。:Y=1.07505+0.00029x,r2=0.99941。1.3.3 。12,3,31,1、231∶1.5、1∶21∶3。,。33.0℃,22.0℃,27.5℃50d。2 2.1 TS、VSpH1。11TSVS11.88%5.43%,213.03%5.13%,312.83%5.67%。TSVS。TSVS,。1 TS、VSpHTable1TS,VSandpHvaluebeforeandafterfermentationTreatmentBeforetreatmentTS∥%VS∥%pHAftertreatmentTS∥%VS∥%pH15.0568.887.64.4565.147.5Experimentalgroup113.5466.317.43.5164.547.5Controlgroup124.7671.897.74.1468.727.5Experimentalgroup223.3463.257.43.2961.347.5Controlgroup233.8274.037.83.3369.837.5Experimentalgroup333.1359.877.33.0158.987.5Controlgroup3,JournalofAnhuiAgri.Sci.2008,36(12):5084-5085,5087 李玮 况玲玲DOI:10.13989/j.cnki.0517-6611.2008.12.0422.2 50d,、1、2。1,,6~8d,。1,6;3,8d,,112ml/d;2。1,33030ml/d,78%,;30,,20%。,,,。2,(30%),。,3433%,。10,12(60%~72%);21;3(56%~66%)。:、(20~30)∶1,、(6∶1),,“”[8-9]。,,,,。1 Fig.1Changeofbiogasproduction2 Fig.2Changetrendofmethanecontent2.3 1,1、2,(2)。2,1,1539ml,,65.12%;3,;2,1151.8ml,11002.2ml31119.8ml,TS54.01%,VS58.35%,。,1∶2,。,1/20,,,。2 Table2ConditionofbiogasproductionTreatment∥mlTotalbiogasproduction∥%AveragemethanecontentTS∥ml/gGasproductionrateofTSVS∥ml/gGasproductionrateofVS1153965.12344.54358.63Experimentalgroup11----Controlgroup12181563.46368.25383.33Experimentalgroup22----Controlgroup23195557.28384.21399.94Experimentalgroup33----Controlgroup3 :“-”,。 Note:-standsforfew,therefore,hasnostatisticalsignificance.3 ,。27.5℃,1∶2,TS368.25ml/g,VS383.33ml/g,63.46%。,50t(),1.8m3。,。,,。,,[10]。。,,、。,,,,、,。[1]何开丽.巢湖富营养化现状与治理对策[J].环境保护,2002(4):22-24.[2]程真文.巢湖藻类污染的研究和对策[J].水处理技术,2003,29(4):247-248.[3]蒋雪英.巢湖“水华”的形成和防治对策[J].内陆水产,2000,12(38):38-39.(下转第5087页)508536卷12期 翟志军等 巢湖蓝藻产沼气的试验研究,,。1 Fig.1 Relationshipbeweengaseouspollutantsconcentrationandal-titude2.1.2 。1,NO2-0.85,,4-0.996,3,NO2(126~165/h,),,,,。,,NO2。NO2,(0.010mg/m3),,NO2,,,,。2.2 2.2.1 。2,5SO2,。,,。,,,,。2.2.2 。3,5、、3NO2,(r=0.82~0.86),3,,,。2,NO2,NO2,,。()NO2,,。2 Fig.2 ChangecurveofSO2concentration3 Fig.3 ChangecurveofNO2concentration3 ,,,()。,。,,,。,,,。,,,。[1]国家环境保护总局.环境空气质量自动监测技术规范(HJ/T193-2005)[S].北京:中国环境科学出版社,2006.(上接第5085页)[4]谢萍,周学文,杨家雄,等.滇池蓝藻对肉仔鸡和生长肥育猪饲用效果研究[J].云南畜牧兽医,2000(2):12-14.[5]董诗旭,董锦艳,宋洪川,等.滇池蓝藻发酵产沼气的研究[J].可再生能源,2006(2):16-18.[6]中科院成都生物研究所.沼气发酵常规分析法[M].1981:1-90.[7]JUHAKALLEM,EINOLARIITTAH,ETTUNENJUKKAA,etal.Responsesofmethaneoxi-dationtotemperatureandwatercontentincoversoilofaboreallandfill[J].SoilBiology&Biochemistry,2007(39):1156-1164.[8]边义,刘庆玉,李金洋.玉米秸秆干发酵制取沼气的试验[J].沈阳农业大学学报,2007,38(3):440-442.[9]HONGWEIYEN,DAVIDE.Anaerobicco-digestionofalgalsludgeandwastepapertoproducemethane[J].BioresourceTechnology,2007,98:130-134.[10]蔡冬清,柳丹,季程晨,等.磁控改性复配物治理水华的研究[J].中国环境学,2007,27(5):661-664.508736卷12期 鲍雷等 重庆缙云山气态污染物梯度对比监测及分析