沉水植物床固定化微生物技术在水源地修复中的应用研究

33120121ENVIRONMENTALSCIENCEVol．33No．1Jan．2012-*210093-．、、5.4×102～2.7×1033.9×105～9.1×1053～24μg·m2·h－119%～74%24%～81%120d．-X173X172A0250-3301201201-0083-052011-01-302011-05-06BK20100562008ZX07101-0122008ZX07101-0041987～[e-mail]*[e-mail]rgedMacrophytesBed-ImmobilizedBacteriainDrinkingWaterRestorationCHENQi-chunLIZheng-kuiWANGYi-chaoWUKaiFANNian-wenStateKeyLaboratoryofPollutantControlandResourcesReuseSchoolofEnvironmentNanjingUniversityNanjing210093ChinaAbstractTheeffectofsubmergedmacrophytesbed-immobilizedbacteriatechnologywhichappliedindrinkingwaterrestorationwasstudied．AmmonifyingbacterianitrobacterianitrosobacteriaanddenitrifyingbacteriawhichisolatedfromTaihuLabewasimmobilizedtotheporouscarriescombinedwiththesubmergedmacrophytesbedtechnologyweappliedthenewequipmentinwaterrestorationofgonghubaythisequipmenthasgoodabilitytoresiststormthedenitrifyingbacterianumberincreasedfrom5.4×102-2.7×103to3.9×105-9.1×105N2Ofluxofexperimentalplotwas3-24μg·m2·h－1it'smorethanthecontrastgroupobviouslyTNconcentrationreduced19%-74%whileNO－3concentrationreduced24%-81%aftertheequipmentrunningaperiodoftimeTheexperimentaldataof120daysshowedthatthistechnologyissuitablefordrinkingwaterrestorationasitcancontroleutrophication．Keywordseutrophicationdrinkingwatersubmergedmacrophytesbed-immobilizedbacteriarestorationdenitrificationVV．．、1～5678～10．、、．11.11-、、．DOI:10.13227/j.hjkx.2012.01.027332010TN1.18～3.44mg·L－1、NH+40～0.58mg·L－1、NO－30.36～0.67mg·L－1、NO－20.0023～0.055mg·L－1、、、、．1Fig．1Sketchmapofdemonstrationproject1.21.2.1、、、、、-β-、－78℃1×104Gy60Co-γ5mm28℃24h3．、、200mL28℃24h、48h、、．1.2.21m×1m×1m、、2cm20m、16m、4m202．2Fig．2Schematicofthesubmergedmacrophytes-bed1.310m×6m642、、、、、、、．1.44ABCD、、．1.51.5.1N-1--、pHpHDO．1.5.2N2O40cm、50cm12V．N2O0900481-19001112130830～1200N2ON2OFF=ρ×VA×ΔcΔt×273273+TFN2ONμg·m2·h－1ρN2O1.25kg/m3Vm3Am2ΔcN2OΔthT14．1.5.315～17．1.6ORIGIN7.5SPSS13.0．22.120107～11、、、、、N2O、DOpH．19%～74%、36%～100%、24%～81%、27%～100%3～6+11．r=-0.771P＜0.053Fig．3DynamicchangesofTNconcentrationintheexperimentdistrict4Fig．4DynamicchangesofNH+4concentrationintheexperimentdistrict5Fig．5DynamicchangesofNO－3concentrationintheexperimentdistrict+、、pHpH58336Fig．6DynamicchangesofNO－2concentrationintheexperimentdistrictr=0.819P＜0.05．2.2pHpH、、CO2pH18pH+9.67．7pHFig．7DynamicchangesofpHvalueintheexperimentdistrict2.3DO729＞＞+＞7298．8DOFig．8DynamicchangesofDOconcentrationintheexperimentdistrict2.4N2O5.4×102～2.7×103+3.9×105～9.1×1052～3SPSSr=0.975P＜0.01－1.95μg·m2·h－111.44μg·m2·h－13～24μg·m2·h－1+9r=－0.78P＜0.05pH10．+-681-9Fig．9N2Ofluxintheexperimentdistrict10Fig．10Dynamicchangesofwatertemperatureintheexperimentdistrict-．31120d+．2、2～3．3N2O．4．1．J．20082982154-2159．2．Acinetobactersp．YY-5J．2009155692-697．3．-J．200930123614-3618．4．/N2OJ．20093072105-2111．5Risgaard-PetersenNMeyerRLRersbechNP．Denitrificationandanaerobicammoniumoxidationinsediments-effectsofmicrophytobenthosandNO－3J．AquaticMicrobialEcology200540167-76．6．J．2008294890-895．7LauridsenTLJensenJPJeppesenEetal．ResponseofsubmergedmacrophytesinDanishlakestonutrientloadingreductionsandbiomanipulationJ．Hydrobiologia20035061-3641-649．8．、J．200516112199-2203．9．J．2009216782-788．10．N、PN2OJ．200727104333-4341．11DongYSZhangSQiYCetal．FluxesofCO2N2OandCH4fromatypicaltemperategrasslandinInnerMongoliaanditsdailyvariationJ．ChineseScienceBulletin200045171590-1594．12BremnerJMRobbinsSGBlackmerAM．SeasonalvariabilityinemissionofnitrousoxidefromsoilJ．GeophysicalResearchLetter198079641-644．13．N2OJ．200223626-30．14．N2OJ．200324133-39．15．M．1985．16．M．1996．17．M．1993．18．J．2007262798-800．78