城市生活污泥蚯蚓处理过程中相关酶活性的动态变化特征

2010,29(5):995-999JournalofAgro-EnvironmentScience2、、、。。4。。X703.1A1672-2043(2010)05-0995-05122221.2100952.225127DynamicChangesofSewageSludgeEnzymeActivitiesDuringVermicompostingXUYi-qun1,2,FENGKe2,WANGZi-bo2,XUJian21.CollegeofResourcesandEnvironmentalSciences,NangjingAgriculturalUniversity,Nangjing210095,China;2.CollegeofEnvironmen－talSciencesandEngineering,YangzhouUniversity,Yangzhou225127,ChinaAbstractLanduseisthetrendforsludgedisposalintheworld.Insoil-sludgesystem,sludgeenzymeplayaimportantroleintheconversionofnutrients.Inthispaper,earthwormswereusedforvermicompostingexperiments,theeffectsofvermicompostingonenzymaticactivities,in－cludingphosphatase,urease,proteaseandinvertaseinsewagesludgewerestudiedduringvermicomposting.Theresultsshowedthattheac－tivitiesofsludgeenzymehadmuchchangeaftertreatedbyearthworms.Theactivitiesofacidicphosphataseandalkalinephosphatasein－creasedintheearlystageofvermicompostingwhereasdecreasedinthelatestage,whiletheactivityofneutralphosphatasekeptdecreaseinthewholestage.Theotherenzymeactivitiesinsewagesludgehadalsochangedaftervermicomposting.Theactivitiesofureaseincreasedintheearlystageofvermicompostingprocesswhereasdecreasedinthelatestage,whiletheactivityofproteaseandinvertasekeptdecreaseinthewholestage.Asawhole,withtime,theactivitiesoftheseenzymedecreasedasorganicmattercontentsdecreased.Asignificantdecreaseoftheactivityofproteasewasobservedascomparedtotheotherenzyme.Theloweractivitiesofenzymeinsewagesludgemaybeduetothedecreaseoforganicmattercontentsaftertreatedbyearthworms.Keywordssewagesludge;earthworm;vermicomposting;enzymeactivity2009－10－0908KJD6100041974—。[e-mail][e-mail]。3000t。、[1]。。。。。N、P20105[2][3]。。ParthasarathiRanganathan[4]。。LeBayonBinet[5]。。、、、。11.12。0.167~0.329g。80.90%319.71g·kg-166.70g·kg-16.12g·kg-16.62g·kg-17.32g·kg-10.62g·kg-11.90g·kg-1pH6.86。4℃。。1.2××=200cm×50cm×35cm30cm。。3d1。。6、8、11、33d、、、4℃。1.3[6]--。[7-8]。1g37℃24h1g37℃24h1g37℃24hNH3-N35-1g37℃24h。。22.1。。1。。6d14.4%17.4%17.7%。6d6~11d。22d20%。[9]996295[10]0.4mg·g-1·d-14mg·g-1·d-1。56%。。2.2。[11]。26d22d1000μg·g-1·d-110[9]。、、。6d。、、、、pH[712-13]。。80%6d71%64%、。。。pH6.44~6.86。。。。2.3、。[10][7]、。[14]。。、[15][16][17]。、pH[8]。3997201056d。、[18-20]。2.4-。422d19%。、[21]。。31、、、。。23。3。4。5、、、。-。[1]ZhengGD,ChenTB,GaoD,etal.Dynamicofleadspeciationinsewagesludgecomposting[J].WaterSciTechnol,2004,5075-82.[2]AyushoM,PascualJA,GarciaC,etal.Evaluationofurbanwastesfora－griculturaluse[J].SoilSciPlantNutr,1996,42105-111.[3]SpinosaL,VesilindPA.Sludgeintobiosolids[M]//Processing,Disposal,Utilization.IWAPublishing,London,UK.2001.[4]ParthasarathiK,RanganathanLS.LongevityofmicrobialandenzymeactivityandtheirinfluenceonNPKcontentinpressmudvermicasts[J].EuropeanJournalofSoilBiology,1999,353107-113.[5]LeBayonRC,BinetF.Earthwormschangethedistributionandavail－abilityofphosphorousinorganicsubstrates[J].SoilBiology&Biochem－istry,2006,38235-246.[6].[M].,198162-142.InstituteofSoilScience.ChineseAcademyofSciences.Analyticalmeth－odsofsoilphysicsandchemistry[M].ShanghaiShanghaiScientificandTechnicalPress,198162-142.[7].[M].,198615-306.GUANSong-yin.Soilenzymeandstudymethod[M].BeijingChinaA－griculturePress,198615-306.[8],.[J].,2005,422306-313.CAIHong,SHENRen-fang.Determinationofsoilproteaseactivitywithmodifiedninhydrincolorimetry[J].ActaPedologicaSinica,2005,422306-313.[9],,,.[J].,2008,1752059-2063.SUNRui-lian,ZHAOBing-qiang,ZHULu-sheng,etal.Dynamicchangesofsoilenzymeactivitiesinlong-termfertilizationsoil[J].E－cologyandEnvironment,2008,1752059-2063.[10],,,.[J].,2008,226162-165.YUEZhong-hui,WANGBo-wen,WANGHong-feng,etal.SeasonaldynamicsofsoilenzymaticactivitiesinWestSongnenAlkaliDegradedGrassland[J].JournalofSoilandWaterConservation,2008,226162-165.[11],.[J].,2004,234633-637.LIYong-hong,GAOYu-bao.Effectsofmonosulfuranonrespiration,hydrogenaseandinvertaseactivityinsoil[J].JournalofAgro-Envi－ronmentalScience,2004,234633-637.[12].[M].,1987129-274.998295ZHOULi-kai.Soilenzymology[M].BeijingSciencePress,1987129-274.[13]KunarV,SinghM.Inhibitionofsoilureaseactivityandnitrificationwithsomemetalliccations[J].AustJSoilRes,1986,244527-532.[14],,,.[J].,2001,216978-981.ZHANGBao-gui,LIGui-tong,SUNZhao,etal.Comparativestudyofdigestiveenzymeactivitiesinearthwormsbelongingtotwodistincteco－logicalcategories[J].ActaEcologicaSinica,2001,216978-981.[15]KaMimuraY,HayanoK.Propertiesofproteaseextractedfromtea-fieldsoil[J].BiologyandFertilityofSoils,2000,30351-355.[16]ZamanM,DiHJ,CameronKC,etal.Grossnitrogenmineralizationandnitrificationratesandtheirrelationshipstoenzymeactivitiesandthesoilmicrobialbiomassinsoilstreatedwithdairyshedeffluentandammoniumfertilizeratdifferentwaterpotentials[J].BiologyandFer－tilityofSoils,1999,29178-186.[17]WatanabeK,HayanoK.Seasonalvariationofsoilproteaseactivitiesandtheirrelationtoproteolyticbacteriaandbacillussppinpaddyfieldsoil[J].SoilBiologyandBiochemistry,1995,272197-203.[18]MishraS,DiHJ,CameronKC,etal.Grossnitrogenmineralisationratesinpasturalsoilsandtheirrelation-shipswithorganicnitrogenfractions,microbialbiomassandproteaseactivityu