城市污水A2O移动床生物膜工艺菌群结构分析肖可可

A2/O11222231．1000842．1000843．214214A2/O、。。MiSeqIllumina、ProteobacteriaBacteroidetes。β-Proteobacter。γ-Proteobacter17．5%β-Proteobacter14%。ProteobacteriaBacteroidetesThiobacillus、ParacoccusPseudomonas。NH3－NNO－2－N1∶2．6。PlanctomycetaceaePlanctomycetales0．0278%。。[e-mail]munityStructureinA2/O－MBBＲSystemforTreatmentofMunicipalWastewaterXIAOKe-ke1ZHOULyu1HEBei-ping2QIANLiang2WANShuo2QULi-ping2HUANGDong-hui31．SchoolofEnvironmentTsinghuaUniversityBeijing100084China2．UnisplendourEnvironmentalProtectionCo．Ltd．Beijing100084China3．JiangsuYulongEnvironmentalProtectionCo．Ltd．Yixing214214ChinaAbstractIntheupgradingprojectusingA2/Oprocessforthetreatmentofmunicipalwastewaterthesuspendedcarriersweredosedintheanaerobicandanoxictankstoimprovethenitrogenandphos-phorusremovalefficiency．Afteroperatingforthreeandahalfyearsthewholesystemshowedimprovednitrogenandphosphorusremovalefficiency．ThecommunitystructureinthesystemwasanalyzedbyMiSeqIlluminasequencingtechniqueandtheresultsshowedthatthedominantmicroorganismsintheanaerobicanoxicandaerobictankswereProteobacteriaandBacteroidetesatphylumlevelandβ-Pro-teobacteratclasslevel．Inthebiofilmformedonthesuspendedcarrierintheanaerobictankthedomi-nantmicroorganismwasProteobacteriaatphylumlevelandγ-Proteobacter17．5%andβ-Proteobacter·02·32920165CHINAWATEＲ＆WASTEWATEＲVol．32No．9May201614%atclasslevel．ThepresenceofProteobacteriaandBacteroidetesmighthavecontributedtothephosphorusremovalefficiency．ThedenitrifyingbacteriaintheanoxictankwerecomposedofThiobacil-lusParacoccusandPseudomonas．ThebiofilmintheanaerobictankshowedaredcolorandhadanAna-mmoxabilitytoremoveammoniumandnitritewitharatioof1∶2．6．TheAnammoxbacteriaPlanctomyc-etaceaeandPlanctomycetalesatfamilyandorderlevelsbothaccountedfor0．0278%．Besidesthedeni-trificationefficiencytheAnammoxpresentintheanaerobictankmighthavealsocontributedtothehighnitrogenremovalefficiencyinthissystem．KeywordsmunicipalwastewaterbiologicalremovalofnitrogenandphosphorusmovingbedbiofilmreactorsuspendedcarrierAnammoxcommunitystructure。。CO2－3、HCO－3CO2。N2N2O。1。A2/O2。//MBBＲ、。MBBＲ。MiSeqIllumina。11.1。37．5×104m3/dA2/O3。HＲT11．0hHＲT2．0hHＲT2．6hHＲT6．4h。3。、、。1.2410%180mL861．8LNH3－NNH4HCO365mg/LNO－2－NNaNO260mg/L。4。100r/min34．2℃1hNH3－NNO－2－N。1.3NO－2－NN－1－－DOCODpHPHS－3EpHTNTP。MiSeqIlluminaDNA、、PCＲ、、、、、、、、、。DNA341b4_F806_ＲDNA5。Mothur＜200bpmaxhomop＞10UsearchOTU97%OTU。、、、、、、。22.120154TN8．07mg/LTN85．7%NH3－N、TP、COD0．81、0．32、22．6mg/LGB18918—2002A、A2/O。·12·．watergasheat．comA2/O3292.21a1bc。C67。1Fig．1BiofilmgrowingonsurfaceofsuspendedcarrierNO－2－N2。4hNH3－NNO－2－NNH3－N65mg/L53．3mg/LNO－2－N60mg/L28mg/L1∶2．661∶2．8～3．6。2NH3－NNO－2－NFig．2ConcentrationsofNH3－NandNO－2－Natdifferentsamplingtime2.397%OTU310。、ProteobacteriaBacteroidetes。3Fig．3Microorganismsdistributionatphylumlevelβ-Proteobacter19．43%、21．2%17．13%4。8·22·329．watergasheat．comProteobacteriaBacteroidetesβ-Proteobacter24%。9Bacteroidetes97%。4Fig．4MicroorganismsdistributionatclasslevelNitrosomonas、NitrosococcusNitrosospiraNitrobacter、Nitrospina、Nitrococ-cusNitrospira。Nitro-somonadalesNO－2－N0．55%Nitro-spira2．04%。Thiobacillusdenitrificans、Micrococcusdenitrificans、Paracoccusdenitrificans、Alkaligenes、Ba-cillusPseudomonas。Thiobacillus0．00265%、Paracoccus0．0345%Pseudomonas0．0822%。VFAsClostridiumaceticum、Acetobacterwoodii、Clos-tridiumtermoautotrophicum、Clostridium、EnterobacteriaClostridium0．029%。β-ProteobacterActinobacteria0．55%0．46%。Okunuki10Actinobacteria。9Actinobacteria。Actinobacteria。Proteobacteriaγ-Proteobacter17．5%β-Pro-teobacter14%Chromatiales13．75%。γ-Proteobacterβ-Proteobacter10。Nitrosomonadales0．055%。Pseudomonas0．0521%Paracoccus0．0382%。CandidatusBrocadiaB．anammoxidans、B．fulgi-da、B．sinica、Anammoxoglobuspropionicus、Jetteniaasiatica、CandidatusKueneniaK．stuttgartiensis、Can-didatusScalinduaS．brodae、S．wagneri、S．sorokinii、S．profundaPlanctomycetes、Planctomyce-talesBrocadiale、PlanctomycetaceaeBrocadi-aceae。PlanctomycetaceaePlanctomyce-·32·．watergasheat．comA2/O329tales0．0278%。5。5Fig．5MicroorganismsdistributionatorderlevelNO－3－N30．28mg/LpH7．08DO0．07～0．09mg/LNO－2－N4．15%1．68%0．77%。Nitro-somonadales0．055%Xu11。。3①、ProteobacteriaBacteroidetes。②Clostridium0．029%Nitrosomonadales0．55%Nitrospira2．04%Thiobacillus0．00265%、Paracoccus0．0345%Pseudomonas0．0822%。③NH3－NNO－2－N1∶2．6。Nitrosomonadales0．055%PlanctomycetaceaePlanctomycetales0．0278%。。1．A2/OJ．201131162－65．2．J．20102025130－132．3．J．201631120－25．4．、pHJ．2006274691－695．5LuYZDingZWDingJetal．Designandevaluationofuniversal16SrＲNAgeneprimersforhigh-throughputsequencingtosimultaneouslydetectDAMOmicrobesand29·42·329．watergasheat．combiofilmreactorandsequencingbatchreactorJ．JHaz-ardMater20111851242－248．6．A/OJ．201329314－16．7Leyva-DiazJCCalderonKＲodriguezFAetal．Com-parativekineticstudybetweenmovingbedbiofilmreac-tor-membranebioreactorandmembranebioreactorsys-temsandtheirinfluenceonorganicmatterandnutrientsremovalJ．BiochemEngJ20137728－40．8．J．201481178－183．9．J．2015351101－109．10APHA．StandardMethodsfortheExaminationofWaterandWastewaterM．WashingtonDCAme