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王鑫1,2,郭书海1,3*,李凤梅1,白玉兴41.,1100162.,1000393.,1100414.,124002:.B0501,CODCr,,pH,CODCr.:B0501CODCr;,30e,pH715,216h,(Q()PQ()5163),CODCr,Q(CODCr)(GB8798-1996).,B0501(Aureobateriumliquefaciens).:;;CODCr;;:X703.1:A:1001-6929(2006)04-0042-05AnalysisofMainAffectingFactorsinMicrobialTreatmentProcessforViscousOilWastewaterWANGXin1,2,GUOShu-hai1,3,LIFeng-mei1,BAIYu-xing41.InstituteofAppliedEcology,ChineseAcademyofSciences,Shenyang110016,China2.GraduateSchoolofChineseAcademyofSciences,Beijing100039,China3.KeyLaboratoryofEnvironmentEngineering,ShenyangUniversity,Shenyang110041,China4.EnvironmentalProtectionEngineerCompany,LiaoheOilfield,Panjin124002,ChinaAbstract:Organicpolymersandpetroleumhydrocarbonsarethemainpollutantsinviscousoilwastewater.ThepredominantstrainB0501wasscreenedfromviscousoilwastewaterfromJincaiSewagePlantofLiaoheOilfieldtoanalyzethefunctionofthestrainontheCODCrremovalrateinthebiologicaltreatmentprocessoftheviscousoilwastewater.Theconditionsincludingtemperature,pH,hydraulicretentiontimeandphosphorusandnitrogennutrientsaffectingtheCODCrremovalrateofviscousoilwastewaterbymicroorganismwereinvestigated.ResultsshowedthattheinoculatingofstrainB0501improvedtheCODCrremovalrate.Undertheconditionsof30e,pH7.5,hydraulicretentiontime216handaddingphosphorusnutrientsmeetingQ(N)BQ(P)ratioof5163,theCODCrremovalratecouldbeimprovedgreatly,andQ(CODCr)valuecouldmeetNationalIntegratedWastewaterDischargeStandards(GB8798-1996).StrainB0501wasidentifiedasAureobateriumliquefaciensaccordingtotheirmorphological,physiologicalandbiochemicalcharacteristics.Keywords:viscousoilwastewater;biologicaltreatment;CODCr;affectingfactors;Aureobateriumliquefaciens:2005-12-03:(863)(2002AA601210);(973)(2004CB418500);(SLYQY0401);(HJGCZDSJJ0406):(1979-),,,.*,,,,90%,[1)2].,.194ResearchofEnvironmentalSciencesVol.19,No.4,2006DOI10.13198/j.res.2006.04.44.wangx.008[3)5].2,,,[6],.,,,[7)8].CODCr,B0501,,pH,CODCr.11.1.B0501,..24h,1.5@108mL-1.1.2[9],[10)11].CODCr,BOD5,,SS,TN,TP[9].1.3500mL,5%(),,,.CODCr,4:¹,,,;º,,CODCr;»,,CODCr;¼,,CODCr.22.1.1.1,,,,.1Table1Theanalyticalresultsofviscousoil1)P(g#cm-3)Pew()P%w()P%w(+)P%w()P%w()P%2)P(mPa#s)0.9771330.114.536.74.070.3057551)20e;2)50e.2.22.2,,Q()Q(CODCr);Q(TN)PQ(TP),.,,,,,.2Table2TheanalysisresultsofwastewatersamplemgPLpHQ()Q(SS)Q(CODCr)Q(BOD5)Q(TN)Q(TP)Q(Cl-)Q(SO42-)Q(Ca2+)Q(Mg2+)7.4710533.623628.5811.820.42507.1010.780.0420.0212.32.3.1[12)15],,,.1,,434:200hQ(CODCr),30e,Q(CODCr).,,,,,;,,.1CODCrFig.1EffectoftemperatureonCODCr2.3.2pHpH,,.pH,pH,.,,..,,pH.2,pH715,CODCr,49177%.2pHCODCrFig.2EffectofpHonCODCrremovalrate2.3.3,,.,,CODCr(3).3CODCrFig.3EffectofHRTonCODCrremovalrate..,,.3,2Q(CODCr),[16].K2Cr2O7,K2Cr2O7,Q(CODCr).,216hQ(CODCr),Q(CODCr).,,.2.3.4,.,,.[17],,.2,Q(TN)PQ(TP),CODCr(4).4,CODCr,CODCr46158%.,Q(),Q()8618215142mgPL,Q()PQ()5.63.,CODCr,Q()PQ()515~6.5,CODCr,CODCr;44194CODCrFig.4EffectofthenutrientsofnitrogenandphosphorusontheCODCrremovalrate,CODCr,46130%.5CODCrFig.5EffectofnutrientsandmicrobeforenhancingtheCODCrremovalrate2.3.5,pH715,Q()PQ()5.63,30e216h,CODCr60.92%,CODCr26163%.,2413%,911%2512%,16194%,37124%31134%.,B0501.2.4,,(016~018)Lm@(215~3)Lm,,,,,(6).D-,.,,,.,.25~30e,pH618~715,.,,.,D-,.,B0501[18)19](Aureobateriumliquefaciens).6B0501(@15000)Fig.6ElectronmicrographofstrainB0501(@15000)3a.B0501(Aureobateriumliquefaciens).b.CODCr.30e,pH715,216h,Q()PQ()5163,CODCr,Q(CODCr)(GB8978-1996).c.CODCr,,CODCr26163%.:[1],,.()[J].,1997,7(1):48)53.PanHonglei,ZhangShiquan,WangDawei.Investigationandevaluationonoperatingsituationofwastewaterprocessingfacilitiesduringproducingperiodinoil&gasfields[J].EnvironmentalProtectionofOil&GasFields,1997,7(1):48)53.[2],,,.[J].,2002,15(1):1)4.GuoShuhai,BaiYuxing,ZhangHairong,etal.Studyonpretreatmentprocessofsuperviscousoilemulsionwastewater[J].ResearchofEnvironmentalSciences,2002,15(1):1)4.[3],,.[J].,2003,23(7):4)7.XieLei,HuYongyou,ZhongHaitao.Oil-bearingwastewatertreatmentprocessesandprogress[J].IndustrialWaterTreatment,2003,23(7):4)7.[4],,.454:[J].,2005,25(7):5)9.HuHao,YanLianhe,WangYing.Presentsituationandprospectoftheoilremovaltechnologyofoil-bearingturbidcirculatingwater[J].IndustrialWaterTreatment,2005,25(7):5)9.[5]BerneF.Physical-chemicalmethodsoftreatmentforoil-containingeffluents[J].WaterSciTechnol,1992,14(9P11):1195)1207.[6],,,.[J].,2003,23(2):157)161.YuanHongli,YangJinshui,WangZhansheng,etal.Microorganismscreeningforpetroleumdegradationanditsdegradingcharacteristics[J].ChinaEnvironmentalScience,2003,23(2):157)161.[7],,,.[J].,2001,21(4):205)208.SuRongguo,MouBozhong,WangXiulin,etal.Biodegradationmechanismandaffectingfactorsofpetroleumhydrocarbons[J].EnvironmentalProtectionofChemicalIndustry,2001,21(4):205)208.[8]JonathanDvanHamme,AjaySingh,OwenPWard.Recentadvancesinpetroleummicrobiology[J].MicrobiolMolBiolRev,2003,67(4):503)549.[9].[M].4.:,2002.StateEnvironmentalProtectionAdministration.Monitoringanddeterminationmethodsforwaterandwastewater[M].4thed.Beijing:ChinaEnvironmentalSciencePress,2002.[10],.[M].:,1991.30)40.PanCui.e,DuTonglin.Petroleumanalysis[M].Shanghai:HuazhongPolytechni